Isolation, Characterization and Biotechnological Potentials of Thraustochytrids from Icelandic Waters
The following study reports on the first thraustochytrid isolates identified from Iceland. They were collected from three different locations off the northern coast of the country (Location A, Skagaströnd; Location B, Hveravík; and Location C, Eyjafjörður). Using 18S rDNA sequence analysis, isolates...
Published in: | Marine Drugs |
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Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
MDPI AG
2019
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Subjects: | |
Online Access: | https://doi.org/10.3390/md17080449 https://doaj.org/article/49ce07fad63040978e4dd6e2dd5d4191 |
Summary: | The following study reports on the first thraustochytrid isolates identified from Iceland. They were collected from three different locations off the northern coast of the country (Location A, Skagaströnd; Location B, Hveravík; and Location C, Eyjafjörður). Using 18S rDNA sequence analysis, isolates from Locations A and B were identified within the Thraustochytrium kinnei species while other isolates within the Sicyoidochytrium minutum species when compared to other known strains. Cells isolated from Locations A ( <math display="inline"> <semantics> <mrow> <mn>2.10</mn> <mo>±</mo> <mn>0.70</mn> </mrow> </semantics> </math> g/L) and B ( <math display="inline"> <semantics> <mrow> <mn>1.54</mn> <mo>±</mo> <mn>0.17</mn> </mrow> </semantics> </math> g/L) produced more biomass than the ones isolated from Location C ( <math display="inline"> <semantics> <mrow> <mn>0.43</mn> <mo>±</mo> <mn>0.02</mn> </mrow> </semantics> </math> g/L). This study offers the first-time examination of the utility of byproducts from fisheries as a nitrogen source in media formulation for thraustochytrids. Experiments showed that isolates produced more biomass (per unit of substrate) when cultured on nitrogen of marine ( <math display="inline"> <semantics> <mrow> <mn>2.55</mn> <mo>±</mo> <mn>0.74</mn> </mrow> </semantics> </math> g/L) as compared to of commercial origin ( <math display="inline"> <semantics> <mrow> <mn>1.06</mn> <mo>±</mo> <mn>0.57</mn> </mrow> </semantics> </math> g/L). Glycerol ( <math display="inline"> <semantics> <mrow> <mn>2.43</mn> <mo>±</mo> <mn>0.56</mn> </mrow> </semantics> </math> g/L) was a ... |
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