The identification and determination of toxin genes of Vibrio strains causing hemorrhagic disease on red drum (Sciaenops ocellatus) using PCR
Abstract Data were collected from 30 strains of Vibrio and sampled on different organs (brain, hemorrhagic site and digestive tract) of Sciaenops ocellatus infection. The results showed that the nucleotide sequences 16S rRNA region are highly similar to those of V. alginolyticus, V. azureus, V. fluv...
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ftdoajarticles:oai:doaj.org/article:49591e2cfd6a43fdbddbe69d8bf4cdaf 2023-05-15T18:06:06+02:00 The identification and determination of toxin genes of Vibrio strains causing hemorrhagic disease on red drum (Sciaenops ocellatus) using PCR Pham Thi Hai Yen Nguyen Quang Linh Nguyen Duy Quynh Tram 2021-01-01T00:00:00Z https://doi.org/10.1186/s13568-020-01161-w https://doaj.org/article/49591e2cfd6a43fdbddbe69d8bf4cdaf EN eng SpringerOpen https://doi.org/10.1186/s13568-020-01161-w https://doaj.org/toc/2191-0855 doi:10.1186/s13568-020-01161-w 2191-0855 https://doaj.org/article/49591e2cfd6a43fdbddbe69d8bf4cdaf AMB Express, Vol 11, Iss 1, Pp 1-8 (2021) Sciaenops ocellatus TDH TRH TLH ToxR Toxin gene Biotechnology TP248.13-248.65 Microbiology QR1-502 article 2021 ftdoajarticles https://doi.org/10.1186/s13568-020-01161-w 2022-12-31T06:44:15Z Abstract Data were collected from 30 strains of Vibrio and sampled on different organs (brain, hemorrhagic site and digestive tract) of Sciaenops ocellatus infection. The results showed that the nucleotide sequences 16S rRNA region are highly similar to those of V. alginolyticus, V. azureus, V. fluvialis, V. natriengens and V. orientalis, which were published on Genbank and other, ranging from 98.05 to 100%. The digestive tract has the most common Vibrio strains (V. alginolyticus [16] V. azureus [7] and V. fluvialis). Thereout, 25 of 30 strains of Vibrio contained 1 to 3 toxin genes, except V. parahaemolyticus. Six parameters were used to measure the DNA polymorphism of 33 homologous DNA sequences in this Vibrio bacteria population. The results indicated that number of separate polymorphic sites (S), total number of mutant sites (Eta), number of haplotype (h), haplotype diversity (Hd), average number of nucleotide differences (k), nucleotide diversity (Pi) were 98 (S), 103 (Eta), 9 (h), 0.887 ± 0.032 (Hd), 25.789 (k) and 17.980 × 10–3 ± 0.003 (Pi), respectively (P < 0,05). The G + C content above 1434 sites positions of nucleotide sequences accounted for 0.542. The phylogenetic tree showed that these strains are divided into six groups. As observed, the appearance of isolated Vibrio on 3 organs of fish (S. ocellatus) hemorrhagic are V. azureus (27,67%), V. alginolyticus (50%), V. orientalis (6,67%) and V. fluvialis (16,67%). Through this result, we found that the diversity of Vibrio species that appeared on the red drum was used in the 16S rRNA region and the presence of toxin genes in these Vibrio species. Article in Journal/Newspaper Red drum Sciaenops ocellatus Directory of Open Access Journals: DOAJ Articles Eta ENVELOPE(-62.917,-62.917,-64.300,-64.300) AMB Express 11 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Sciaenops ocellatus TDH TRH TLH ToxR Toxin gene Biotechnology TP248.13-248.65 Microbiology QR1-502 |
spellingShingle |
Sciaenops ocellatus TDH TRH TLH ToxR Toxin gene Biotechnology TP248.13-248.65 Microbiology QR1-502 Pham Thi Hai Yen Nguyen Quang Linh Nguyen Duy Quynh Tram The identification and determination of toxin genes of Vibrio strains causing hemorrhagic disease on red drum (Sciaenops ocellatus) using PCR |
topic_facet |
Sciaenops ocellatus TDH TRH TLH ToxR Toxin gene Biotechnology TP248.13-248.65 Microbiology QR1-502 |
description |
Abstract Data were collected from 30 strains of Vibrio and sampled on different organs (brain, hemorrhagic site and digestive tract) of Sciaenops ocellatus infection. The results showed that the nucleotide sequences 16S rRNA region are highly similar to those of V. alginolyticus, V. azureus, V. fluvialis, V. natriengens and V. orientalis, which were published on Genbank and other, ranging from 98.05 to 100%. The digestive tract has the most common Vibrio strains (V. alginolyticus [16] V. azureus [7] and V. fluvialis). Thereout, 25 of 30 strains of Vibrio contained 1 to 3 toxin genes, except V. parahaemolyticus. Six parameters were used to measure the DNA polymorphism of 33 homologous DNA sequences in this Vibrio bacteria population. The results indicated that number of separate polymorphic sites (S), total number of mutant sites (Eta), number of haplotype (h), haplotype diversity (Hd), average number of nucleotide differences (k), nucleotide diversity (Pi) were 98 (S), 103 (Eta), 9 (h), 0.887 ± 0.032 (Hd), 25.789 (k) and 17.980 × 10–3 ± 0.003 (Pi), respectively (P < 0,05). The G + C content above 1434 sites positions of nucleotide sequences accounted for 0.542. The phylogenetic tree showed that these strains are divided into six groups. As observed, the appearance of isolated Vibrio on 3 organs of fish (S. ocellatus) hemorrhagic are V. azureus (27,67%), V. alginolyticus (50%), V. orientalis (6,67%) and V. fluvialis (16,67%). Through this result, we found that the diversity of Vibrio species that appeared on the red drum was used in the 16S rRNA region and the presence of toxin genes in these Vibrio species. |
format |
Article in Journal/Newspaper |
author |
Pham Thi Hai Yen Nguyen Quang Linh Nguyen Duy Quynh Tram |
author_facet |
Pham Thi Hai Yen Nguyen Quang Linh Nguyen Duy Quynh Tram |
author_sort |
Pham Thi Hai Yen |
title |
The identification and determination of toxin genes of Vibrio strains causing hemorrhagic disease on red drum (Sciaenops ocellatus) using PCR |
title_short |
The identification and determination of toxin genes of Vibrio strains causing hemorrhagic disease on red drum (Sciaenops ocellatus) using PCR |
title_full |
The identification and determination of toxin genes of Vibrio strains causing hemorrhagic disease on red drum (Sciaenops ocellatus) using PCR |
title_fullStr |
The identification and determination of toxin genes of Vibrio strains causing hemorrhagic disease on red drum (Sciaenops ocellatus) using PCR |
title_full_unstemmed |
The identification and determination of toxin genes of Vibrio strains causing hemorrhagic disease on red drum (Sciaenops ocellatus) using PCR |
title_sort |
identification and determination of toxin genes of vibrio strains causing hemorrhagic disease on red drum (sciaenops ocellatus) using pcr |
publisher |
SpringerOpen |
publishDate |
2021 |
url |
https://doi.org/10.1186/s13568-020-01161-w https://doaj.org/article/49591e2cfd6a43fdbddbe69d8bf4cdaf |
long_lat |
ENVELOPE(-62.917,-62.917,-64.300,-64.300) |
geographic |
Eta |
geographic_facet |
Eta |
genre |
Red drum Sciaenops ocellatus |
genre_facet |
Red drum Sciaenops ocellatus |
op_source |
AMB Express, Vol 11, Iss 1, Pp 1-8 (2021) |
op_relation |
https://doi.org/10.1186/s13568-020-01161-w https://doaj.org/toc/2191-0855 doi:10.1186/s13568-020-01161-w 2191-0855 https://doaj.org/article/49591e2cfd6a43fdbddbe69d8bf4cdaf |
op_doi |
https://doi.org/10.1186/s13568-020-01161-w |
container_title |
AMB Express |
container_volume |
11 |
container_issue |
1 |
_version_ |
1766177669489098752 |