Hepatotoxicity and oxidative stress induced byNaja haje crude venom
Background Snake venoms are synthesized and stored in venom glands. Most venoms are complex mixtures of several proteins, peptides, enzymes, toxins and non-protein components. In the present study, we investigated the oxidative stress and apoptosis in rat liver cells provoked by Naja haje crude inje...
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ftdoajarticles:oai:doaj.org/article:4954640c62ac4b8e8c10b790ed5fc2b6 2023-05-15T15:10:53+02:00 Hepatotoxicity and oxidative stress induced byNaja haje crude venom Saleh Al-Quraishy Mahamed A Dkhil Ahmed Esmat Abdel Moneim 2014-10-01T00:00:00Z https://doi.org/10.1186/1678-9199-20-42 https://doaj.org/article/4954640c62ac4b8e8c10b790ed5fc2b6 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200314&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1186/1678-9199-20-42 https://doaj.org/article/4954640c62ac4b8e8c10b790ed5fc2b6 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 20, Iss 0, Pp 1-10 (2014) Naja haje venom Hepatotoxicity Oxidative stress Apoptosis Rats Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2014 ftdoajarticles https://doi.org/10.1186/1678-9199-20-42 2022-12-31T01:36:54Z Background Snake venoms are synthesized and stored in venom glands. Most venoms are complex mixtures of several proteins, peptides, enzymes, toxins and non-protein components. In the present study, we investigated the oxidative stress and apoptosis in rat liver cells provoked by Naja haje crude injection (LD50) after four hours.Methods Wistar rats were randomly divided into two groups, the control group was intraperitoneally injected with saline solution while LD50-dose envenomed group was intraperitoneally injected with venom at a dose of 0.025 μg/kg of body weight. Animals were killed four hours after the injection. Lipid peroxidation, nitric oxide and glutathione levels were measured as oxidative markers in serum and liver homogenate. In addition, liver function parameters and activities of antioxidant enzymes were determined.ResultsN. haje crude venom (0.025 μg/kg of body weight) enhanced lipid peroxidation and nitric oxide production in both serum and liver with concomitant reduction in glutathione, catalase, glutathione reductase and glutathione-S-transferase activities. Superoxide dismutase and glutathione peroxidase activities were significantly increased in liver of envenomed rats. These findings were associated with apoptosis induction in the liver. In addition, N. haje crude venom caused hepatic injury as indicated by histopathological changes in the liver tissue with an elevation in total bilirubin, serum alanine aminotransferase, aspartate aminotransferase, γ-glutamyl transpeptidase, and alkaline phosphatase.Conclusions Based on the present results, it can hypothesized that N. haje crude venom is a potent inducer of toxin-mediated hepatotoxicity associated with apoptosis in the liver. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 20 1 42 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
Naja haje venom Hepatotoxicity Oxidative stress Apoptosis Rats Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Naja haje venom Hepatotoxicity Oxidative stress Apoptosis Rats Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Saleh Al-Quraishy Mahamed A Dkhil Ahmed Esmat Abdel Moneim Hepatotoxicity and oxidative stress induced byNaja haje crude venom |
topic_facet |
Naja haje venom Hepatotoxicity Oxidative stress Apoptosis Rats Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Background Snake venoms are synthesized and stored in venom glands. Most venoms are complex mixtures of several proteins, peptides, enzymes, toxins and non-protein components. In the present study, we investigated the oxidative stress and apoptosis in rat liver cells provoked by Naja haje crude injection (LD50) after four hours.Methods Wistar rats were randomly divided into two groups, the control group was intraperitoneally injected with saline solution while LD50-dose envenomed group was intraperitoneally injected with venom at a dose of 0.025 μg/kg of body weight. Animals were killed four hours after the injection. Lipid peroxidation, nitric oxide and glutathione levels were measured as oxidative markers in serum and liver homogenate. In addition, liver function parameters and activities of antioxidant enzymes were determined.ResultsN. haje crude venom (0.025 μg/kg of body weight) enhanced lipid peroxidation and nitric oxide production in both serum and liver with concomitant reduction in glutathione, catalase, glutathione reductase and glutathione-S-transferase activities. Superoxide dismutase and glutathione peroxidase activities were significantly increased in liver of envenomed rats. These findings were associated with apoptosis induction in the liver. In addition, N. haje crude venom caused hepatic injury as indicated by histopathological changes in the liver tissue with an elevation in total bilirubin, serum alanine aminotransferase, aspartate aminotransferase, γ-glutamyl transpeptidase, and alkaline phosphatase.Conclusions Based on the present results, it can hypothesized that N. haje crude venom is a potent inducer of toxin-mediated hepatotoxicity associated with apoptosis in the liver. |
format |
Article in Journal/Newspaper |
author |
Saleh Al-Quraishy Mahamed A Dkhil Ahmed Esmat Abdel Moneim |
author_facet |
Saleh Al-Quraishy Mahamed A Dkhil Ahmed Esmat Abdel Moneim |
author_sort |
Saleh Al-Quraishy |
title |
Hepatotoxicity and oxidative stress induced byNaja haje crude venom |
title_short |
Hepatotoxicity and oxidative stress induced byNaja haje crude venom |
title_full |
Hepatotoxicity and oxidative stress induced byNaja haje crude venom |
title_fullStr |
Hepatotoxicity and oxidative stress induced byNaja haje crude venom |
title_full_unstemmed |
Hepatotoxicity and oxidative stress induced byNaja haje crude venom |
title_sort |
hepatotoxicity and oxidative stress induced bynaja haje crude venom |
publisher |
SciELO |
publishDate |
2014 |
url |
https://doi.org/10.1186/1678-9199-20-42 https://doaj.org/article/4954640c62ac4b8e8c10b790ed5fc2b6 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 20, Iss 0, Pp 1-10 (2014) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200314&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1186/1678-9199-20-42 https://doaj.org/article/4954640c62ac4b8e8c10b790ed5fc2b6 |
op_doi |
https://doi.org/10.1186/1678-9199-20-42 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
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20 |
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1 |
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42 |
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