The effects of different strength of MS media in solid and liquid media on in vitro growth of Typhonium flagelliforme
Objective: To determine the effects of different strength of Murashige and Skoog (MS) media (full, ½ and ¼) in solid and liquid media on in vitro growth of Typhonium flagelliforme (T. flagelliforme), whereby an optimum media composition can be provided for mass propagation of T. flagelliforme. Metho...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Wolters Kluwer Medknow Publications
2017
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Online Access: | https://doi.org/10.1016/j.apjtb.2016.11.019 https://doaj.org/article/4924246693a84b4480b969e630d4911c |
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ftdoajarticles:oai:doaj.org/article:4924246693a84b4480b969e630d4911c 2023-05-15T15:14:07+02:00 The effects of different strength of MS media in solid and liquid media on in vitro growth of Typhonium flagelliforme Nur Inani Rezali Norrizah Jaafar Sidik Azani Saleh Nurul Izzati Osman Nurul Athirah Mohd Adam 2017-02-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2016.11.019 https://doaj.org/article/4924246693a84b4480b969e630d4911c EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169116302805 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2016.11.019 https://doaj.org/article/4924246693a84b4480b969e630d4911c Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 2, Pp 151-156 (2017) Plant tissue culture Micropropagation Typhonium flagelliforme L MS media strength Solid culture media Liquid culture media Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2017 ftdoajarticles https://doi.org/10.1016/j.apjtb.2016.11.019 2022-12-31T12:19:53Z Objective: To determine the effects of different strength of Murashige and Skoog (MS) media (full, ½ and ¼) in solid and liquid media on in vitro growth of Typhonium flagelliforme (T. flagelliforme), whereby an optimum media composition can be provided for mass propagation of T. flagelliforme. Methods: Rhizome bud of T. flagelliforme was obtained from the axenic in vitro established T. flagelliforme plantlets in Plant Tissue Culture Laboratory, Universiti Teknologi MARA, Shah Alam. Rhizome bud was used as explant and cultured onto shoot proliferation medium under different strength of MS media (full, ½, ¼) in solid and liquid culture media. Results: After 6 weeks of culture, the number of shoot, number of leaf, number of root, height of shoot, fresh weight, dry weight and chlorophyll content of T. flagelliforme were analyzed. A comparison was made between liquid and solid culture media. The results revealed that the liquid culture media were more effective for all the growth parameters (shoot height, shoot number, leaf number, root number, fresh weight, dry weight, chlorophyll a and chlorophyll b content) compared to solid culture media. Apart from that, this study revealed the positive relationship between strength of MS media and type of culture media (solid and liquid media) to the growth of T. flagelliforme. Growth of T. flagelliforme was improved when MS strength was increased in liquid media. In contrast, growth of T. flagelliforme was improved when MS strength was decreased in solid media. Conclusions: Through this study, an optimum media composition for mass propagation of T. flagelliforme had been established by observing effects of MS media strength and type of culture media (solid and liquid media) on the growth of T. flagelliforme. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Mara ENVELOPE(132.133,132.133,62.267,62.267) Asian Pacific Journal of Tropical Biomedicine 7 2 151 156 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Plant tissue culture Micropropagation Typhonium flagelliforme L MS media strength Solid culture media Liquid culture media Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Plant tissue culture Micropropagation Typhonium flagelliforme L MS media strength Solid culture media Liquid culture media Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Nur Inani Rezali Norrizah Jaafar Sidik Azani Saleh Nurul Izzati Osman Nurul Athirah Mohd Adam The effects of different strength of MS media in solid and liquid media on in vitro growth of Typhonium flagelliforme |
topic_facet |
Plant tissue culture Micropropagation Typhonium flagelliforme L MS media strength Solid culture media Liquid culture media Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To determine the effects of different strength of Murashige and Skoog (MS) media (full, ½ and ¼) in solid and liquid media on in vitro growth of Typhonium flagelliforme (T. flagelliforme), whereby an optimum media composition can be provided for mass propagation of T. flagelliforme. Methods: Rhizome bud of T. flagelliforme was obtained from the axenic in vitro established T. flagelliforme plantlets in Plant Tissue Culture Laboratory, Universiti Teknologi MARA, Shah Alam. Rhizome bud was used as explant and cultured onto shoot proliferation medium under different strength of MS media (full, ½, ¼) in solid and liquid culture media. Results: After 6 weeks of culture, the number of shoot, number of leaf, number of root, height of shoot, fresh weight, dry weight and chlorophyll content of T. flagelliforme were analyzed. A comparison was made between liquid and solid culture media. The results revealed that the liquid culture media were more effective for all the growth parameters (shoot height, shoot number, leaf number, root number, fresh weight, dry weight, chlorophyll a and chlorophyll b content) compared to solid culture media. Apart from that, this study revealed the positive relationship between strength of MS media and type of culture media (solid and liquid media) to the growth of T. flagelliforme. Growth of T. flagelliforme was improved when MS strength was increased in liquid media. In contrast, growth of T. flagelliforme was improved when MS strength was decreased in solid media. Conclusions: Through this study, an optimum media composition for mass propagation of T. flagelliforme had been established by observing effects of MS media strength and type of culture media (solid and liquid media) on the growth of T. flagelliforme. |
format |
Article in Journal/Newspaper |
author |
Nur Inani Rezali Norrizah Jaafar Sidik Azani Saleh Nurul Izzati Osman Nurul Athirah Mohd Adam |
author_facet |
Nur Inani Rezali Norrizah Jaafar Sidik Azani Saleh Nurul Izzati Osman Nurul Athirah Mohd Adam |
author_sort |
Nur Inani Rezali |
title |
The effects of different strength of MS media in solid and liquid media on in vitro growth of Typhonium flagelliforme |
title_short |
The effects of different strength of MS media in solid and liquid media on in vitro growth of Typhonium flagelliforme |
title_full |
The effects of different strength of MS media in solid and liquid media on in vitro growth of Typhonium flagelliforme |
title_fullStr |
The effects of different strength of MS media in solid and liquid media on in vitro growth of Typhonium flagelliforme |
title_full_unstemmed |
The effects of different strength of MS media in solid and liquid media on in vitro growth of Typhonium flagelliforme |
title_sort |
effects of different strength of ms media in solid and liquid media on in vitro growth of typhonium flagelliforme |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2017 |
url |
https://doi.org/10.1016/j.apjtb.2016.11.019 https://doaj.org/article/4924246693a84b4480b969e630d4911c |
long_lat |
ENVELOPE(132.133,132.133,62.267,62.267) |
geographic |
Arctic Mara |
geographic_facet |
Arctic Mara |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 2, Pp 151-156 (2017) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169116302805 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2016.11.019 https://doaj.org/article/4924246693a84b4480b969e630d4911c |
op_doi |
https://doi.org/10.1016/j.apjtb.2016.11.019 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
7 |
container_issue |
2 |
container_start_page |
151 |
op_container_end_page |
156 |
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1766344611513499648 |