Bacterial communities of Aedes aegypti mosquitoes differ between crop and midgut tissues.
Microbiota studies of Aedes aegypti and other mosquitoes generally focus on the bacterial communities found in adult female midguts. However, other compartments of the digestive tract maintain communities of bacteria which remain almost entirely unstudied. For example, the Dipteran crop is a food st...
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ftdoajarticles:oai:doaj.org/article:48f3b83c415d4bce8199eee25514d77a 2023-06-11T04:09:57+02:00 Bacterial communities of Aedes aegypti mosquitoes differ between crop and midgut tissues. Luis E Martinez Villegas James Radl George Dimopoulos Sarah M Short 2023-03-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0011218 https://doaj.org/article/48f3b83c415d4bce8199eee25514d77a EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0011218 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0011218 https://doaj.org/article/48f3b83c415d4bce8199eee25514d77a PLoS Neglected Tropical Diseases, Vol 17, Iss 3, p e0011218 (2023) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2023 ftdoajarticles https://doi.org/10.1371/journal.pntd.0011218 2023-05-07T00:31:57Z Microbiota studies of Aedes aegypti and other mosquitoes generally focus on the bacterial communities found in adult female midguts. However, other compartments of the digestive tract maintain communities of bacteria which remain almost entirely unstudied. For example, the Dipteran crop is a food storage organ, but few studies have looked at the microbiome of crops in mosquitoes, and only a single previous study has investigated the crop in Ae. aegypti. In this study, we used both culture-dependent and culture-independent methods to compare the bacterial communities in midguts and crops of laboratory reared Ae. aegypti. Both methods revealed a trend towards higher abundance, but also higher variability, of bacteria in the midgut than the crop. When present, bacteria from the genus Elizabethkingia (family Weeksellaceae) dominated midgut bacterial communities. In crops, we found a higher diversity of bacteria, and these communities were generally dominated by acetic acid bacteria (family Acetobacteriaceae) from the genera Tanticharoenia and Asaia. These three taxa drove significant community structure differences between the tissues. We used FAPROTAX to predict the metabolic functions of these communities and found that crop bacterial communities were significantly more likely to contain bacteria capable of methanol oxidation and methylotrophy. Both the presence of acetic acid bacteria (which commonly catabolize sugar to produce acetic acid) and the functional profile that includes methanol oxidation (which is correlated with bacteria found with natural sources like nectar) may relate to the presence of sugar, which is stored in the mosquito crop. A better understanding of what bacteria are present in the digestive tract of mosquitoes and how these communities assemble will inform how the microbiota impacts mosquito physiology and the full spectrum of functions provided by the microbiota. It may also facilitate better methods of engineering the mosquito microbiome for vector control or prevention of disease ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 17 3 e0011218 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Luis E Martinez Villegas James Radl George Dimopoulos Sarah M Short Bacterial communities of Aedes aegypti mosquitoes differ between crop and midgut tissues. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Microbiota studies of Aedes aegypti and other mosquitoes generally focus on the bacterial communities found in adult female midguts. However, other compartments of the digestive tract maintain communities of bacteria which remain almost entirely unstudied. For example, the Dipteran crop is a food storage organ, but few studies have looked at the microbiome of crops in mosquitoes, and only a single previous study has investigated the crop in Ae. aegypti. In this study, we used both culture-dependent and culture-independent methods to compare the bacterial communities in midguts and crops of laboratory reared Ae. aegypti. Both methods revealed a trend towards higher abundance, but also higher variability, of bacteria in the midgut than the crop. When present, bacteria from the genus Elizabethkingia (family Weeksellaceae) dominated midgut bacterial communities. In crops, we found a higher diversity of bacteria, and these communities were generally dominated by acetic acid bacteria (family Acetobacteriaceae) from the genera Tanticharoenia and Asaia. These three taxa drove significant community structure differences between the tissues. We used FAPROTAX to predict the metabolic functions of these communities and found that crop bacterial communities were significantly more likely to contain bacteria capable of methanol oxidation and methylotrophy. Both the presence of acetic acid bacteria (which commonly catabolize sugar to produce acetic acid) and the functional profile that includes methanol oxidation (which is correlated with bacteria found with natural sources like nectar) may relate to the presence of sugar, which is stored in the mosquito crop. A better understanding of what bacteria are present in the digestive tract of mosquitoes and how these communities assemble will inform how the microbiota impacts mosquito physiology and the full spectrum of functions provided by the microbiota. It may also facilitate better methods of engineering the mosquito microbiome for vector control or prevention of disease ... |
format |
Article in Journal/Newspaper |
author |
Luis E Martinez Villegas James Radl George Dimopoulos Sarah M Short |
author_facet |
Luis E Martinez Villegas James Radl George Dimopoulos Sarah M Short |
author_sort |
Luis E Martinez Villegas |
title |
Bacterial communities of Aedes aegypti mosquitoes differ between crop and midgut tissues. |
title_short |
Bacterial communities of Aedes aegypti mosquitoes differ between crop and midgut tissues. |
title_full |
Bacterial communities of Aedes aegypti mosquitoes differ between crop and midgut tissues. |
title_fullStr |
Bacterial communities of Aedes aegypti mosquitoes differ between crop and midgut tissues. |
title_full_unstemmed |
Bacterial communities of Aedes aegypti mosquitoes differ between crop and midgut tissues. |
title_sort |
bacterial communities of aedes aegypti mosquitoes differ between crop and midgut tissues. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2023 |
url |
https://doi.org/10.1371/journal.pntd.0011218 https://doaj.org/article/48f3b83c415d4bce8199eee25514d77a |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 17, Iss 3, p e0011218 (2023) |
op_relation |
https://doi.org/10.1371/journal.pntd.0011218 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0011218 https://doaj.org/article/48f3b83c415d4bce8199eee25514d77a |
op_doi |
https://doi.org/10.1371/journal.pntd.0011218 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
17 |
container_issue |
3 |
container_start_page |
e0011218 |
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1768383984558931968 |