Isolation, Characterisation, and Lipase Production of a Cold-Adapted Bacterial Strain Pseudomonas sp. LSK25 Isolated from Signy Island, Antarctica
In recent years, studies on psychrophilic lipases have been an emerging area of research in the field of enzymology. This study focuses on bacterial strains isolated from anthropogenically-influenced soil samples collected around Signy Island Research Station (South Orkney Islands, maritime Antarcti...
Published in: | Molecules |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
MDPI AG
2019
|
Subjects: | |
Online Access: | https://doi.org/10.3390/molecules24040715 https://doaj.org/article/48c1a750fff9437797037fe9496ade58 |
id |
ftdoajarticles:oai:doaj.org/article:48c1a750fff9437797037fe9496ade58 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:48c1a750fff9437797037fe9496ade58 2023-05-15T14:00:35+02:00 Isolation, Characterisation, and Lipase Production of a Cold-Adapted Bacterial Strain Pseudomonas sp. LSK25 Isolated from Signy Island, Antarctica Leelatulasi Salwoom Raja Noor Zaliha Raja Abd Rahman Abu Bakar Salleh Fairolniza Mohd. Shariff Peter Convey David Pearce Mohd Shukuri Mohamad Ali 2019-02-01T00:00:00Z https://doi.org/10.3390/molecules24040715 https://doaj.org/article/48c1a750fff9437797037fe9496ade58 EN eng MDPI AG https://www.mdpi.com/1420-3049/24/4/715 https://doaj.org/toc/1420-3049 1420-3049 doi:10.3390/molecules24040715 https://doaj.org/article/48c1a750fff9437797037fe9496ade58 Molecules, Vol 24, Iss 4, p 715 (2019) Antarctica cold adapted lipase Pseudomonas sp. LSK25 lipase production Organic chemistry QD241-441 article 2019 ftdoajarticles https://doi.org/10.3390/molecules24040715 2022-12-31T02:44:09Z In recent years, studies on psychrophilic lipases have been an emerging area of research in the field of enzymology. This study focuses on bacterial strains isolated from anthropogenically-influenced soil samples collected around Signy Island Research Station (South Orkney Islands, maritime Antarctic). Limited information on lipase activities from bacteria isolated from Signy station is currently available. The presence of lipase genes was determined using real time quantification PCR (qPCR) in samples obtained from three different locations on Signy Island. Twenty strains from the location with highest lipase gene detection were screened for lipolytic activities at a temperature of 4 °C, and from this one strain was selected for further examination based on the highest enzymatic activities obtained. Analysis of 16S rRNA sequence data of this strain showed the highest level of sequence similarity (98%) to a Pseudomonas sp. strain also isolated from Antarctica. In order to increase lipase production of this psychrophilic strain, optimisation of different parameters of physical and nutritional factors were investigated. Optimal production was obtained at 10 °C and pH 7.0, at 150 rev/min shaking rate over 36 h incubation. Article in Journal/Newspaper Antarc* Antarctic Antarctica Signy Island South Orkney Islands Directory of Open Access Journals: DOAJ Articles Antarctic South Orkney Islands ENVELOPE(-45.500,-45.500,-60.583,-60.583) Signy Island ENVELOPE(-45.595,-45.595,-60.708,-60.708) Signy Station ENVELOPE(-45.600,-45.600,-60.717,-60.717) Molecules 24 4 715 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Antarctica cold adapted lipase Pseudomonas sp. LSK25 lipase production Organic chemistry QD241-441 |
spellingShingle |
Antarctica cold adapted lipase Pseudomonas sp. LSK25 lipase production Organic chemistry QD241-441 Leelatulasi Salwoom Raja Noor Zaliha Raja Abd Rahman Abu Bakar Salleh Fairolniza Mohd. Shariff Peter Convey David Pearce Mohd Shukuri Mohamad Ali Isolation, Characterisation, and Lipase Production of a Cold-Adapted Bacterial Strain Pseudomonas sp. LSK25 Isolated from Signy Island, Antarctica |
topic_facet |
Antarctica cold adapted lipase Pseudomonas sp. LSK25 lipase production Organic chemistry QD241-441 |
description |
In recent years, studies on psychrophilic lipases have been an emerging area of research in the field of enzymology. This study focuses on bacterial strains isolated from anthropogenically-influenced soil samples collected around Signy Island Research Station (South Orkney Islands, maritime Antarctic). Limited information on lipase activities from bacteria isolated from Signy station is currently available. The presence of lipase genes was determined using real time quantification PCR (qPCR) in samples obtained from three different locations on Signy Island. Twenty strains from the location with highest lipase gene detection were screened for lipolytic activities at a temperature of 4 °C, and from this one strain was selected for further examination based on the highest enzymatic activities obtained. Analysis of 16S rRNA sequence data of this strain showed the highest level of sequence similarity (98%) to a Pseudomonas sp. strain also isolated from Antarctica. In order to increase lipase production of this psychrophilic strain, optimisation of different parameters of physical and nutritional factors were investigated. Optimal production was obtained at 10 °C and pH 7.0, at 150 rev/min shaking rate over 36 h incubation. |
format |
Article in Journal/Newspaper |
author |
Leelatulasi Salwoom Raja Noor Zaliha Raja Abd Rahman Abu Bakar Salleh Fairolniza Mohd. Shariff Peter Convey David Pearce Mohd Shukuri Mohamad Ali |
author_facet |
Leelatulasi Salwoom Raja Noor Zaliha Raja Abd Rahman Abu Bakar Salleh Fairolniza Mohd. Shariff Peter Convey David Pearce Mohd Shukuri Mohamad Ali |
author_sort |
Leelatulasi Salwoom |
title |
Isolation, Characterisation, and Lipase Production of a Cold-Adapted Bacterial Strain Pseudomonas sp. LSK25 Isolated from Signy Island, Antarctica |
title_short |
Isolation, Characterisation, and Lipase Production of a Cold-Adapted Bacterial Strain Pseudomonas sp. LSK25 Isolated from Signy Island, Antarctica |
title_full |
Isolation, Characterisation, and Lipase Production of a Cold-Adapted Bacterial Strain Pseudomonas sp. LSK25 Isolated from Signy Island, Antarctica |
title_fullStr |
Isolation, Characterisation, and Lipase Production of a Cold-Adapted Bacterial Strain Pseudomonas sp. LSK25 Isolated from Signy Island, Antarctica |
title_full_unstemmed |
Isolation, Characterisation, and Lipase Production of a Cold-Adapted Bacterial Strain Pseudomonas sp. LSK25 Isolated from Signy Island, Antarctica |
title_sort |
isolation, characterisation, and lipase production of a cold-adapted bacterial strain pseudomonas sp. lsk25 isolated from signy island, antarctica |
publisher |
MDPI AG |
publishDate |
2019 |
url |
https://doi.org/10.3390/molecules24040715 https://doaj.org/article/48c1a750fff9437797037fe9496ade58 |
long_lat |
ENVELOPE(-45.500,-45.500,-60.583,-60.583) ENVELOPE(-45.595,-45.595,-60.708,-60.708) ENVELOPE(-45.600,-45.600,-60.717,-60.717) |
geographic |
Antarctic South Orkney Islands Signy Island Signy Station |
geographic_facet |
Antarctic South Orkney Islands Signy Island Signy Station |
genre |
Antarc* Antarctic Antarctica Signy Island South Orkney Islands |
genre_facet |
Antarc* Antarctic Antarctica Signy Island South Orkney Islands |
op_source |
Molecules, Vol 24, Iss 4, p 715 (2019) |
op_relation |
https://www.mdpi.com/1420-3049/24/4/715 https://doaj.org/toc/1420-3049 1420-3049 doi:10.3390/molecules24040715 https://doaj.org/article/48c1a750fff9437797037fe9496ade58 |
op_doi |
https://doi.org/10.3390/molecules24040715 |
container_title |
Molecules |
container_volume |
24 |
container_issue |
4 |
container_start_page |
715 |
_version_ |
1766269807611609088 |