MOLECULAR TYPING OF Giardia duodenalis ISOLATES FROM NONHUMAN PRIMATES HOUSED IN A BRAZILIAN ZOO
Giardia infections in captive nonhuman primates (NHP) housed at a Brazilian zoo were investigated in order to address their zoonotic potential. Fresh fecal samples were collected from the floors of 22 enclosures where 47 primates of 18 different species were housed. The diagnosis of intestinal paras...
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ftdoajarticles:oai:doaj.org/article:48b66f8f79704d8eaac54b563adf991e 2024-09-09T19:27:06+00:00 MOLECULAR TYPING OF Giardia duodenalis ISOLATES FROM NONHUMAN PRIMATES HOUSED IN A BRAZILIAN ZOO Erica Boarato David Mariella Patti Silvana Torossian Coradi Teresa Cristina Goulart Oliveira-Sequeira Paulo Eduardo Martins Ribolla Semiramis Guimaraes 2014-01-01T00:00:00Z https://doi.org/10.1590/S0036-46652014000100007 https://doaj.org/article/48b66f8f79704d8eaac54b563adf991e EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652014000100049&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S0036-46652014000100007 https://doaj.org/article/48b66f8f79704d8eaac54b563adf991e Revista do Instituto de Medicina Tropical de São Paulo, Vol 56, Iss 1, Pp 49-54 (2014) Giardia duodenalis Molecular characterization Nonhuman primates Zoo Zoonosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2014 ftdoajarticles https://doi.org/10.1590/S0036-46652014000100007 2024-08-05T17:49:31Z Giardia infections in captive nonhuman primates (NHP) housed at a Brazilian zoo were investigated in order to address their zoonotic potential. Fresh fecal samples were collected from the floors of 22 enclosures where 47 primates of 18 different species were housed. The diagnosis of intestinal parasites after concentration by sedimentation and flotation methods revealed the following parasites and their frequencies: Giardia (18%); Entamoeba spp. (18%); Endolimax nana (4.5%); Iodamoeba spp. (4.5%); Oxyurid (4.5%) and Strongylid (4.5%). Genomic DNA extracted from all samples was processed by PCR methods in order to amplify fragments of gdh and tpi genes of Giardia. Amplicons were obtained from samples of Ateles belzebuth, Alouatta caraya, Alouatta fusca and Alouatta seniculus. Clear sequences were only obtained for the isolates from Ateles belzebuth (BA1), Alouatta fusca (BA2) and Alouatta caraya (BA3). According to the phenetic analyses of these sequences, all were classified as assemblage A. For the tpi gene, all three isolates were grouped into sub-assemblage AII (BA1, BA2 and BA3) whereas for the gdh gene, only BA3 was sub-assemblage AII, and the BA1 and BA2 were sub-assemblage AI. Considering the zoonotic potential of the assemblage A, and that the animals of the present study show no clinical signs of infection, the data obtained here stresses that regular coproparasitological surveys are necessary to implement preventive measures and safeguard the health of the captive animals, of their caretakers and of people visiting the zoological gardens. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 56 1 49 54 |
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English |
topic |
Giardia duodenalis Molecular characterization Nonhuman primates Zoo Zoonosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Giardia duodenalis Molecular characterization Nonhuman primates Zoo Zoonosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Erica Boarato David Mariella Patti Silvana Torossian Coradi Teresa Cristina Goulart Oliveira-Sequeira Paulo Eduardo Martins Ribolla Semiramis Guimaraes MOLECULAR TYPING OF Giardia duodenalis ISOLATES FROM NONHUMAN PRIMATES HOUSED IN A BRAZILIAN ZOO |
topic_facet |
Giardia duodenalis Molecular characterization Nonhuman primates Zoo Zoonosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Giardia infections in captive nonhuman primates (NHP) housed at a Brazilian zoo were investigated in order to address their zoonotic potential. Fresh fecal samples were collected from the floors of 22 enclosures where 47 primates of 18 different species were housed. The diagnosis of intestinal parasites after concentration by sedimentation and flotation methods revealed the following parasites and their frequencies: Giardia (18%); Entamoeba spp. (18%); Endolimax nana (4.5%); Iodamoeba spp. (4.5%); Oxyurid (4.5%) and Strongylid (4.5%). Genomic DNA extracted from all samples was processed by PCR methods in order to amplify fragments of gdh and tpi genes of Giardia. Amplicons were obtained from samples of Ateles belzebuth, Alouatta caraya, Alouatta fusca and Alouatta seniculus. Clear sequences were only obtained for the isolates from Ateles belzebuth (BA1), Alouatta fusca (BA2) and Alouatta caraya (BA3). According to the phenetic analyses of these sequences, all were classified as assemblage A. For the tpi gene, all three isolates were grouped into sub-assemblage AII (BA1, BA2 and BA3) whereas for the gdh gene, only BA3 was sub-assemblage AII, and the BA1 and BA2 were sub-assemblage AI. Considering the zoonotic potential of the assemblage A, and that the animals of the present study show no clinical signs of infection, the data obtained here stresses that regular coproparasitological surveys are necessary to implement preventive measures and safeguard the health of the captive animals, of their caretakers and of people visiting the zoological gardens. |
format |
Article in Journal/Newspaper |
author |
Erica Boarato David Mariella Patti Silvana Torossian Coradi Teresa Cristina Goulart Oliveira-Sequeira Paulo Eduardo Martins Ribolla Semiramis Guimaraes |
author_facet |
Erica Boarato David Mariella Patti Silvana Torossian Coradi Teresa Cristina Goulart Oliveira-Sequeira Paulo Eduardo Martins Ribolla Semiramis Guimaraes |
author_sort |
Erica Boarato David |
title |
MOLECULAR TYPING OF Giardia duodenalis ISOLATES FROM NONHUMAN PRIMATES HOUSED IN A BRAZILIAN ZOO |
title_short |
MOLECULAR TYPING OF Giardia duodenalis ISOLATES FROM NONHUMAN PRIMATES HOUSED IN A BRAZILIAN ZOO |
title_full |
MOLECULAR TYPING OF Giardia duodenalis ISOLATES FROM NONHUMAN PRIMATES HOUSED IN A BRAZILIAN ZOO |
title_fullStr |
MOLECULAR TYPING OF Giardia duodenalis ISOLATES FROM NONHUMAN PRIMATES HOUSED IN A BRAZILIAN ZOO |
title_full_unstemmed |
MOLECULAR TYPING OF Giardia duodenalis ISOLATES FROM NONHUMAN PRIMATES HOUSED IN A BRAZILIAN ZOO |
title_sort |
molecular typing of giardia duodenalis isolates from nonhuman primates housed in a brazilian zoo |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2014 |
url |
https://doi.org/10.1590/S0036-46652014000100007 https://doaj.org/article/48b66f8f79704d8eaac54b563adf991e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 56, Iss 1, Pp 49-54 (2014) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652014000100049&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S0036-46652014000100007 https://doaj.org/article/48b66f8f79704d8eaac54b563adf991e |
op_doi |
https://doi.org/10.1590/S0036-46652014000100007 |
container_title |
Revista do Instituto de Medicina Tropical de São Paulo |
container_volume |
56 |
container_issue |
1 |
container_start_page |
49 |
op_container_end_page |
54 |
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1809896596122370048 |