Multiple species‐specific molecular markers using nanofluidic array as a tool to detect prey DNA from carnivore scats
Abstract Large carnivore feeding ecology plays a crucial role for management and conservation for predators and their prey. One of the keys to this kind of research is to identify the species composition in the predator diet, for example, prey determination from scat content. DNA‐based methods appli...
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ftdoajarticles:oai:doaj.org/article:489deb694ff34607b08884f42e9c0be3 2023-05-15T15:49:54+02:00 Multiple species‐specific molecular markers using nanofluidic array as a tool to detect prey DNA from carnivore scats Cecilia Di Bernardi Camilla Wikenros Eva Hedmark Luigi Boitani Paolo Ciucci Håkan Sand Mikael Åkesson 2021-09-01T00:00:00Z https://doi.org/10.1002/ece3.7918 https://doaj.org/article/489deb694ff34607b08884f42e9c0be3 EN eng Wiley https://doi.org/10.1002/ece3.7918 https://doaj.org/toc/2045-7758 2045-7758 doi:10.1002/ece3.7918 https://doaj.org/article/489deb694ff34607b08884f42e9c0be3 Ecology and Evolution, Vol 11, Iss 17, Pp 11739-11748 (2021) Canis lupus carnivore cytochrome b gene diet assessment prey identification species detection Ecology QH540-549.5 article 2021 ftdoajarticles https://doi.org/10.1002/ece3.7918 2022-12-31T14:33:05Z Abstract Large carnivore feeding ecology plays a crucial role for management and conservation for predators and their prey. One of the keys to this kind of research is to identify the species composition in the predator diet, for example, prey determination from scat content. DNA‐based methods applied to detect prey in predators’ scats are viable alternatives to traditional macroscopic approaches, showing an increased reliability and higher prey detection rate. Here, we developed a molecular method for prey species identification in wolf (Canis lupus) scats using multiple species‐specific marker loci on the cytochrome b gene for 18 target species. The final panel consisted of 80 assays, with a minimum of four markers per target species, and that amplified specifically when using a high‐throughput Nanofluidic array technology (Fluidigm Inc.). As a practical example, we applied the method to identify target prey species DNA in 80 wolf scats collected in Sweden. Depending on the number of amplifying markers required to obtain a positive species call in a scat, the success in determining at least one prey species from the scats ranged from 44% to 92%. Although we highlight the need to evaluate the optimal number of markers for sensitive target species detection, the developed method is a fast and cost‐efficient tool for prey identification in wolf scats and it also has the potential to be further developed and applied to other areas and large carnivores as well. Article in Journal/Newspaper Canis lupus Directory of Open Access Journals: DOAJ Articles Ecology and Evolution 11 17 11739 11748 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Canis lupus carnivore cytochrome b gene diet assessment prey identification species detection Ecology QH540-549.5 |
spellingShingle |
Canis lupus carnivore cytochrome b gene diet assessment prey identification species detection Ecology QH540-549.5 Cecilia Di Bernardi Camilla Wikenros Eva Hedmark Luigi Boitani Paolo Ciucci Håkan Sand Mikael Åkesson Multiple species‐specific molecular markers using nanofluidic array as a tool to detect prey DNA from carnivore scats |
topic_facet |
Canis lupus carnivore cytochrome b gene diet assessment prey identification species detection Ecology QH540-549.5 |
description |
Abstract Large carnivore feeding ecology plays a crucial role for management and conservation for predators and their prey. One of the keys to this kind of research is to identify the species composition in the predator diet, for example, prey determination from scat content. DNA‐based methods applied to detect prey in predators’ scats are viable alternatives to traditional macroscopic approaches, showing an increased reliability and higher prey detection rate. Here, we developed a molecular method for prey species identification in wolf (Canis lupus) scats using multiple species‐specific marker loci on the cytochrome b gene for 18 target species. The final panel consisted of 80 assays, with a minimum of four markers per target species, and that amplified specifically when using a high‐throughput Nanofluidic array technology (Fluidigm Inc.). As a practical example, we applied the method to identify target prey species DNA in 80 wolf scats collected in Sweden. Depending on the number of amplifying markers required to obtain a positive species call in a scat, the success in determining at least one prey species from the scats ranged from 44% to 92%. Although we highlight the need to evaluate the optimal number of markers for sensitive target species detection, the developed method is a fast and cost‐efficient tool for prey identification in wolf scats and it also has the potential to be further developed and applied to other areas and large carnivores as well. |
format |
Article in Journal/Newspaper |
author |
Cecilia Di Bernardi Camilla Wikenros Eva Hedmark Luigi Boitani Paolo Ciucci Håkan Sand Mikael Åkesson |
author_facet |
Cecilia Di Bernardi Camilla Wikenros Eva Hedmark Luigi Boitani Paolo Ciucci Håkan Sand Mikael Åkesson |
author_sort |
Cecilia Di Bernardi |
title |
Multiple species‐specific molecular markers using nanofluidic array as a tool to detect prey DNA from carnivore scats |
title_short |
Multiple species‐specific molecular markers using nanofluidic array as a tool to detect prey DNA from carnivore scats |
title_full |
Multiple species‐specific molecular markers using nanofluidic array as a tool to detect prey DNA from carnivore scats |
title_fullStr |
Multiple species‐specific molecular markers using nanofluidic array as a tool to detect prey DNA from carnivore scats |
title_full_unstemmed |
Multiple species‐specific molecular markers using nanofluidic array as a tool to detect prey DNA from carnivore scats |
title_sort |
multiple species‐specific molecular markers using nanofluidic array as a tool to detect prey dna from carnivore scats |
publisher |
Wiley |
publishDate |
2021 |
url |
https://doi.org/10.1002/ece3.7918 https://doaj.org/article/489deb694ff34607b08884f42e9c0be3 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Ecology and Evolution, Vol 11, Iss 17, Pp 11739-11748 (2021) |
op_relation |
https://doi.org/10.1002/ece3.7918 https://doaj.org/toc/2045-7758 2045-7758 doi:10.1002/ece3.7918 https://doaj.org/article/489deb694ff34607b08884f42e9c0be3 |
op_doi |
https://doi.org/10.1002/ece3.7918 |
container_title |
Ecology and Evolution |
container_volume |
11 |
container_issue |
17 |
container_start_page |
11739 |
op_container_end_page |
11748 |
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1766384911132917760 |