Comparative analysis of virulence determinants, phylogroups, and antibiotic susceptibility patterns of typical versus atypical Enteroaggregative E. coli in India.
Enteroaggregative Escherichia coli (EAEC) is an evolving enteric pathogen that causes acute and chronic diarrhea in developed and industrialized nations in children. EAEC epidemiology and the importance of atypical EAEC (aEAEC) isolation in childhood diarrhea are not well documented in the Indian se...
Published in: | PLOS Neglected Tropical Diseases |
---|---|
Main Authors: | , , , , , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2020
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pntd.0008769 https://doaj.org/article/48835a703c7d4cf798f7cdf36a14a9fd |
id |
ftdoajarticles:oai:doaj.org/article:48835a703c7d4cf798f7cdf36a14a9fd |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:48835a703c7d4cf798f7cdf36a14a9fd 2023-05-15T15:16:41+02:00 Comparative analysis of virulence determinants, phylogroups, and antibiotic susceptibility patterns of typical versus atypical Enteroaggregative E. coli in India. Vinay Modgil Jaspreet Mahindroo Chandradeo Narayan Manmohit Kalia Md Yousuf Varun Shahi Meenakshi Koundal Pankaj Chaudhary Ruby Jain Kawaljeet Singh Sandha Seema Tanwar Pratima Gupta Kamlesh Thakur Digvijay Singh Neha Gautam Manish Kakkar Bhavneet Bharti Balvinder Mohan Neelam Taneja 2020-11-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008769 https://doaj.org/article/48835a703c7d4cf798f7cdf36a14a9fd EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008769 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008769 https://doaj.org/article/48835a703c7d4cf798f7cdf36a14a9fd PLoS Neglected Tropical Diseases, Vol 14, Iss 11, p e0008769 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008769 2022-12-31T13:53:10Z Enteroaggregative Escherichia coli (EAEC) is an evolving enteric pathogen that causes acute and chronic diarrhea in developed and industrialized nations in children. EAEC epidemiology and the importance of atypical EAEC (aEAEC) isolation in childhood diarrhea are not well documented in the Indian setting. A comparative analysis was undertaken to evaluate virulence, phylogeny, and antibiotic sensitivity among typical tEAEC versus aEAEC. A total of 171 EAEC isolates were extracted from a broad surveillance sample of diarrheal (N = 1210) and healthy children (N = 550) across North India. Polymerase chain reaction (PCR) for the aggR gene (master regulator gene) was conducted to differentiate tEAEC and aEAEC. For 21 virulence genes, we used multiplex PCR to classify possible virulence factors among these strains. Phylogenetic classes were identified by a multiplex PCR for chuA, yjaA, and a cryptic DNA fragment, TspE4C2. Antibiotic susceptibility was conducted by the disc diffusion method as per CLSI guidelines. EAEC was associated with moderate to severe diarrhea in children. The prevalence of EAEC infection (11.4%) was higher than any other DEC group (p = 0.002). tEAEC occurrence in the diarrheal group was higher than in the control group (p = 0.0001). tEAEC strain harbored more virulence genes than aEAEC. astA, aap, and aggR genes were most frequently found in the EAEC from the diarrheal population. Within tEAEC, this gene combination was present in more than 50% of strains. Also, 75.8% of EAEC strains were multidrug-resistant (MDR). Phylogroup D (43.9%) and B1 (39.4%) were most prevalent in the diarrheal and control group, respectively. Genetic analysis revealed EAEC variability; the comparison of tEAEC and aEAEC allowed us to better understand the EAEC virulence repertoire. Further microbiological and epidemiological research is required to examine the pathogenicity of not only typical but also atypical EAEC. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Indian PLOS Neglected Tropical Diseases 14 11 e0008769 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Vinay Modgil Jaspreet Mahindroo Chandradeo Narayan Manmohit Kalia Md Yousuf Varun Shahi Meenakshi Koundal Pankaj Chaudhary Ruby Jain Kawaljeet Singh Sandha Seema Tanwar Pratima Gupta Kamlesh Thakur Digvijay Singh Neha Gautam Manish Kakkar Bhavneet Bharti Balvinder Mohan Neelam Taneja Comparative analysis of virulence determinants, phylogroups, and antibiotic susceptibility patterns of typical versus atypical Enteroaggregative E. coli in India. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Enteroaggregative Escherichia coli (EAEC) is an evolving enteric pathogen that causes acute and chronic diarrhea in developed and industrialized nations in children. EAEC epidemiology and the importance of atypical EAEC (aEAEC) isolation in childhood diarrhea are not well documented in the Indian setting. A comparative analysis was undertaken to evaluate virulence, phylogeny, and antibiotic sensitivity among typical tEAEC versus aEAEC. A total of 171 EAEC isolates were extracted from a broad surveillance sample of diarrheal (N = 1210) and healthy children (N = 550) across North India. Polymerase chain reaction (PCR) for the aggR gene (master regulator gene) was conducted to differentiate tEAEC and aEAEC. For 21 virulence genes, we used multiplex PCR to classify possible virulence factors among these strains. Phylogenetic classes were identified by a multiplex PCR for chuA, yjaA, and a cryptic DNA fragment, TspE4C2. Antibiotic susceptibility was conducted by the disc diffusion method as per CLSI guidelines. EAEC was associated with moderate to severe diarrhea in children. The prevalence of EAEC infection (11.4%) was higher than any other DEC group (p = 0.002). tEAEC occurrence in the diarrheal group was higher than in the control group (p = 0.0001). tEAEC strain harbored more virulence genes than aEAEC. astA, aap, and aggR genes were most frequently found in the EAEC from the diarrheal population. Within tEAEC, this gene combination was present in more than 50% of strains. Also, 75.8% of EAEC strains were multidrug-resistant (MDR). Phylogroup D (43.9%) and B1 (39.4%) were most prevalent in the diarrheal and control group, respectively. Genetic analysis revealed EAEC variability; the comparison of tEAEC and aEAEC allowed us to better understand the EAEC virulence repertoire. Further microbiological and epidemiological research is required to examine the pathogenicity of not only typical but also atypical EAEC. |
format |
Article in Journal/Newspaper |
author |
Vinay Modgil Jaspreet Mahindroo Chandradeo Narayan Manmohit Kalia Md Yousuf Varun Shahi Meenakshi Koundal Pankaj Chaudhary Ruby Jain Kawaljeet Singh Sandha Seema Tanwar Pratima Gupta Kamlesh Thakur Digvijay Singh Neha Gautam Manish Kakkar Bhavneet Bharti Balvinder Mohan Neelam Taneja |
author_facet |
Vinay Modgil Jaspreet Mahindroo Chandradeo Narayan Manmohit Kalia Md Yousuf Varun Shahi Meenakshi Koundal Pankaj Chaudhary Ruby Jain Kawaljeet Singh Sandha Seema Tanwar Pratima Gupta Kamlesh Thakur Digvijay Singh Neha Gautam Manish Kakkar Bhavneet Bharti Balvinder Mohan Neelam Taneja |
author_sort |
Vinay Modgil |
title |
Comparative analysis of virulence determinants, phylogroups, and antibiotic susceptibility patterns of typical versus atypical Enteroaggregative E. coli in India. |
title_short |
Comparative analysis of virulence determinants, phylogroups, and antibiotic susceptibility patterns of typical versus atypical Enteroaggregative E. coli in India. |
title_full |
Comparative analysis of virulence determinants, phylogroups, and antibiotic susceptibility patterns of typical versus atypical Enteroaggregative E. coli in India. |
title_fullStr |
Comparative analysis of virulence determinants, phylogroups, and antibiotic susceptibility patterns of typical versus atypical Enteroaggregative E. coli in India. |
title_full_unstemmed |
Comparative analysis of virulence determinants, phylogroups, and antibiotic susceptibility patterns of typical versus atypical Enteroaggregative E. coli in India. |
title_sort |
comparative analysis of virulence determinants, phylogroups, and antibiotic susceptibility patterns of typical versus atypical enteroaggregative e. coli in india. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0008769 https://doaj.org/article/48835a703c7d4cf798f7cdf36a14a9fd |
geographic |
Arctic Indian |
geographic_facet |
Arctic Indian |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 11, p e0008769 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008769 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008769 https://doaj.org/article/48835a703c7d4cf798f7cdf36a14a9fd |
op_doi |
https://doi.org/10.1371/journal.pntd.0008769 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
14 |
container_issue |
11 |
container_start_page |
e0008769 |
_version_ |
1766346974648336384 |