Poseidonibacter ostreae sp. nov., Isolated from the Gut of Ostrea from the Seomjin River

Three Gram-negative strains, SJOD-M-6 T , SJOD-M-5, and SJOD-M-33, were isolated from Ostrea denselamellosa . These strains are oxidase- and catalase-positive coccoids that thrive aerobically. The three strains shared 100.0% 16S rRNA gene sequence similarity and showed average nucleotide identity (A...

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Bibliographic Details
Published in:Diversity
Main Authors: Kiwoon Baek, Sumin Jang, Eu Jin Chung, Shi Hyun Ryu, Ahyoung Choi
Format: Article in Journal/Newspaper
Language:English
Published: MDPI AG 2023
Subjects:
Poseidonibacter ostreae
Ostrea denselamellosa
new taxa
Biology (General)
QH301-705.5
Antarc*
antarcticus
Online Access:https://doi.org/10.3390/d15080920
https://doaj.org/article/48781aa620e7433c93d4459d72c6c72a
Description
Summary:Three Gram-negative strains, SJOD-M-6 T , SJOD-M-5, and SJOD-M-33, were isolated from Ostrea denselamellosa . These strains are oxidase- and catalase-positive coccoids that thrive aerobically. The three strains shared 100.0% 16S rRNA gene sequence similarity and showed average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values of 99.7–99.8% and 93.8–96.8%, suggesting that they belonged to the same species. Phylogenetic analysis based on the 16S rRNA gene revealed that all three isolates belong to the genus Poseidonibacter . Their closest neighbors were Poseidonibacter parvus LPB0137 T (98.8%), Poseidonibacter antarcticus SM1702 T (98.7%), and Poseidonibacter lekithochrous LFT 1.7 T (95.5%). However, the ANI and dDDH values between SJOD-M-6 T (the representative strain of the novel species) and its closest phylogenetic relatives fell well below the established cut-off values of <95% (ANI) and <70% (dDDH) for species delineation. Furthermore, several phenotypic traits distinguish the novel strains from their closest relatives. Based on the combined genotypic and phenotypic data, strains SJOD-M-6 T , SJOD-M-5, and SJOD-M-33 are classified as novel species within the genus Poseidonibacter . We propose the name Poseidonibacter ostreae sp. nov. for this novel species, with the type strain being SJOD-M-6 T (=KCTC 72758 T = NBRC 114334 T = FBCC-B685).

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