Primary Structure and Coding Genes of Two Pheromones from the Antarctic Psychrophilic Ciliate, Euplotes focardii

• Published in: Microorganisms
• Main Authors: Claudio Alimenti, Annalisa Candelori, Yaohan Jiang, Pierangelo Luporini, Adriana Vallesi
• Format: Article in Journal/Newspaper
• Language: English
• Published: MDPI AG 2022
• Subjects: protein pheromones; macronuclear gene structures; polar microbiology; water-borne chemical signals; Biology (General); QH301-705.5; Antarctic; The Antarctic; Antarc*
• Online Access: https://doi.org/10.3390/microorganisms10061089; https://doaj.org/article/486f3eea78c04a8dbb561d4a862d90a1

In ciliates, diffusible cell type-specific pheromones regulate cell growth and mating phenomena acting competitively in both autocrine and heterologous fashion. In Euplotes species, these signaling molecules are represented by species-specific families of structurally homologous small, disulfide-rich proteins, each specified by one of a series of multiple alleles that are inherited without relationships of dominance at the mat -genetic locus of the germinal micronuclear genome, and expressed as individual gene-sized molecules in the somatic macronuclear genome. Here we report the 85-amino acid sequences and the full-length macronuclear nucleotide coding sequences of two pheromones, designated E f -1 and E f -2, isolated from the supernatant of a wild-type strain of a psychrophilic species of Euplotes , E. focardii , endemic to Antarctic coastal waters. An overall comparison of the determined E. focardii pheromone and pheromone-gene structures with their homologs from congeneric species provides an initial picture of how an evolutionary increase in the complexity of these structures accompanies Euplotes speciation.