Tick-borne encephalitis virus inhibits rRNA synthesis and host protein production in human cells of neural origin.
Tick-borne encephalitis virus (TBEV), a member of the genus Flavivirus (Flaviviridae), is a causative agent of a severe neuroinfection. Recently, several flaviviruses have been shown to interact with host protein synthesis. In order to determine whether TBEV interacts with this host process in its n...
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ftdoajarticles:oai:doaj.org/article:485609578341417ea7cf057bd8cf8eb1 2023-05-15T15:04:02+02:00 Tick-borne encephalitis virus inhibits rRNA synthesis and host protein production in human cells of neural origin. Martin Selinger Hana Tykalová Ján Štěrba Pavlína Věchtová Zuzana Vavrušková Jaroslava Lieskovská Alain Kohl Esther Schnettler Libor Grubhoffer 2019-09-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0007745 https://doaj.org/article/485609578341417ea7cf057bd8cf8eb1 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0007745 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007745 https://doaj.org/article/485609578341417ea7cf057bd8cf8eb1 PLoS Neglected Tropical Diseases, Vol 13, Iss 9, p e0007745 (2019) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2019 ftdoajarticles https://doi.org/10.1371/journal.pntd.0007745 2022-12-31T04:02:48Z Tick-borne encephalitis virus (TBEV), a member of the genus Flavivirus (Flaviviridae), is a causative agent of a severe neuroinfection. Recently, several flaviviruses have been shown to interact with host protein synthesis. In order to determine whether TBEV interacts with this host process in its natural target cells, we analysed de novo protein synthesis in a human cell line derived from cerebellar medulloblastoma (DAOY HTB-186). We observed a significant decrease in the rate of host protein synthesis, including the housekeeping genes HPRT1 and GAPDH and the known interferon-stimulated gene viperin. In addition, TBEV infection resulted in a specific decrease of RNA polymerase I (POLR1) transcripts, 18S and 28S rRNAs and their precursor, 45-47S pre-rRNA, but had no effect on the POLR3 transcribed 5S rRNA levels. To our knowledge, this is the first report of flavivirus-induced decrease of specifically POLR1 rRNA transcripts accompanied by host translational shut-off. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 13 9 e0007745 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Martin Selinger Hana Tykalová Ján Štěrba Pavlína Věchtová Zuzana Vavrušková Jaroslava Lieskovská Alain Kohl Esther Schnettler Libor Grubhoffer Tick-borne encephalitis virus inhibits rRNA synthesis and host protein production in human cells of neural origin. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Tick-borne encephalitis virus (TBEV), a member of the genus Flavivirus (Flaviviridae), is a causative agent of a severe neuroinfection. Recently, several flaviviruses have been shown to interact with host protein synthesis. In order to determine whether TBEV interacts with this host process in its natural target cells, we analysed de novo protein synthesis in a human cell line derived from cerebellar medulloblastoma (DAOY HTB-186). We observed a significant decrease in the rate of host protein synthesis, including the housekeeping genes HPRT1 and GAPDH and the known interferon-stimulated gene viperin. In addition, TBEV infection resulted in a specific decrease of RNA polymerase I (POLR1) transcripts, 18S and 28S rRNAs and their precursor, 45-47S pre-rRNA, but had no effect on the POLR3 transcribed 5S rRNA levels. To our knowledge, this is the first report of flavivirus-induced decrease of specifically POLR1 rRNA transcripts accompanied by host translational shut-off. |
format |
Article in Journal/Newspaper |
author |
Martin Selinger Hana Tykalová Ján Štěrba Pavlína Věchtová Zuzana Vavrušková Jaroslava Lieskovská Alain Kohl Esther Schnettler Libor Grubhoffer |
author_facet |
Martin Selinger Hana Tykalová Ján Štěrba Pavlína Věchtová Zuzana Vavrušková Jaroslava Lieskovská Alain Kohl Esther Schnettler Libor Grubhoffer |
author_sort |
Martin Selinger |
title |
Tick-borne encephalitis virus inhibits rRNA synthesis and host protein production in human cells of neural origin. |
title_short |
Tick-borne encephalitis virus inhibits rRNA synthesis and host protein production in human cells of neural origin. |
title_full |
Tick-borne encephalitis virus inhibits rRNA synthesis and host protein production in human cells of neural origin. |
title_fullStr |
Tick-borne encephalitis virus inhibits rRNA synthesis and host protein production in human cells of neural origin. |
title_full_unstemmed |
Tick-borne encephalitis virus inhibits rRNA synthesis and host protein production in human cells of neural origin. |
title_sort |
tick-borne encephalitis virus inhibits rrna synthesis and host protein production in human cells of neural origin. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2019 |
url |
https://doi.org/10.1371/journal.pntd.0007745 https://doaj.org/article/485609578341417ea7cf057bd8cf8eb1 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 13, Iss 9, p e0007745 (2019) |
op_relation |
https://doi.org/10.1371/journal.pntd.0007745 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007745 https://doaj.org/article/485609578341417ea7cf057bd8cf8eb1 |
op_doi |
https://doi.org/10.1371/journal.pntd.0007745 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
13 |
container_issue |
9 |
container_start_page |
e0007745 |
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1766335859688210432 |