Attenuation of lipopolysaccharide-induced neuroinflammatory events in BV-2 microglial cells by Moringa oleifera leaf extract
Objective: To determine the anti-neuroinflammatory activity of Moringa oleifera leaf extract (MLE) under lipopolysaccharide stimulation of mouse murine microglia BV2 cells in vitro. Methods: The cytotoxicity effect of MLE was investigated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium br...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Wolters Kluwer Medknow Publications
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ftdoajarticles:oai:doaj.org/article:48444daf58cb4208814ee82d328cce56 2023-05-15T15:09:02+02:00 Attenuation of lipopolysaccharide-induced neuroinflammatory events in BV-2 microglial cells by Moringa oleifera leaf extract Gothai Sivaprakasam Palanivel Ganesan Katyakyini Muniandy Shin-Young Park Duk-Yeon Cho Joon-So Kim Palanisamy Arulselvan Dong-Kug Choi 2019-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.254604 https://doaj.org/article/48444daf58cb4208814ee82d328cce56 EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2019;volume=9;issue=3;spage=109;epage=115;aulast=Sivaprakasam https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.254604 https://doaj.org/article/48444daf58cb4208814ee82d328cce56 Asian Pacific Journal of Tropical Biomedicine, Vol 9, Iss 3, Pp 109-115 (2019) moringa oleifera leaf extract bv2 microglial cells neuro-inflammation pro-inflammatory cytokines nf-κb signaling pathway Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2019 ftdoajarticles https://doi.org/10.4103/2221-1691.254604 2022-12-31T10:11:04Z Objective: To determine the anti-neuroinflammatory activity of Moringa oleifera leaf extract (MLE) under lipopolysaccharide stimulation of mouse murine microglia BV2 cells in vitro. Methods: The cytotoxicity effect of MLE was investigated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide assay. The inflammatory response of BV-2 cells were induced with lipopolysaccharide. The generation of nitric oxide levels was determined by using Griess assay and the level of pro-inflammatory cytokines (IL-1β, IL-6 and TNF-α) was evaluated by ELISA kit. The expression of iNOS, COX-2 as well as IκB-α was carried out by immunoblot analysis. Results: MLE reduced the nitric oxide production in concentration-dependent manner, and maintained the viability of BV-2 microglial cells which indicated absence of toxicity. In addition, MLE repressed the activation of nuclear factor kappa B by arresting the deterioration of IκB-α, consequently resulted in suppression of cytokines expression such as COX-2 and iNOS. Conclusions: MLE inhibitory activities are associated with the inhibition of nuclear factor kappa B transcriptional activity in BV2 microglial cells. Thus MLE may offer a substantial treatment for neuroinflammatory diseases. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 9 3 109 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
moringa oleifera leaf extract bv2 microglial cells neuro-inflammation pro-inflammatory cytokines nf-κb signaling pathway Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
moringa oleifera leaf extract bv2 microglial cells neuro-inflammation pro-inflammatory cytokines nf-κb signaling pathway Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Gothai Sivaprakasam Palanivel Ganesan Katyakyini Muniandy Shin-Young Park Duk-Yeon Cho Joon-So Kim Palanisamy Arulselvan Dong-Kug Choi Attenuation of lipopolysaccharide-induced neuroinflammatory events in BV-2 microglial cells by Moringa oleifera leaf extract |
topic_facet |
moringa oleifera leaf extract bv2 microglial cells neuro-inflammation pro-inflammatory cytokines nf-κb signaling pathway Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To determine the anti-neuroinflammatory activity of Moringa oleifera leaf extract (MLE) under lipopolysaccharide stimulation of mouse murine microglia BV2 cells in vitro. Methods: The cytotoxicity effect of MLE was investigated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide assay. The inflammatory response of BV-2 cells were induced with lipopolysaccharide. The generation of nitric oxide levels was determined by using Griess assay and the level of pro-inflammatory cytokines (IL-1β, IL-6 and TNF-α) was evaluated by ELISA kit. The expression of iNOS, COX-2 as well as IκB-α was carried out by immunoblot analysis. Results: MLE reduced the nitric oxide production in concentration-dependent manner, and maintained the viability of BV-2 microglial cells which indicated absence of toxicity. In addition, MLE repressed the activation of nuclear factor kappa B by arresting the deterioration of IκB-α, consequently resulted in suppression of cytokines expression such as COX-2 and iNOS. Conclusions: MLE inhibitory activities are associated with the inhibition of nuclear factor kappa B transcriptional activity in BV2 microglial cells. Thus MLE may offer a substantial treatment for neuroinflammatory diseases. |
format |
Article in Journal/Newspaper |
author |
Gothai Sivaprakasam Palanivel Ganesan Katyakyini Muniandy Shin-Young Park Duk-Yeon Cho Joon-So Kim Palanisamy Arulselvan Dong-Kug Choi |
author_facet |
Gothai Sivaprakasam Palanivel Ganesan Katyakyini Muniandy Shin-Young Park Duk-Yeon Cho Joon-So Kim Palanisamy Arulselvan Dong-Kug Choi |
author_sort |
Gothai Sivaprakasam |
title |
Attenuation of lipopolysaccharide-induced neuroinflammatory events in BV-2 microglial cells by Moringa oleifera leaf extract |
title_short |
Attenuation of lipopolysaccharide-induced neuroinflammatory events in BV-2 microglial cells by Moringa oleifera leaf extract |
title_full |
Attenuation of lipopolysaccharide-induced neuroinflammatory events in BV-2 microglial cells by Moringa oleifera leaf extract |
title_fullStr |
Attenuation of lipopolysaccharide-induced neuroinflammatory events in BV-2 microglial cells by Moringa oleifera leaf extract |
title_full_unstemmed |
Attenuation of lipopolysaccharide-induced neuroinflammatory events in BV-2 microglial cells by Moringa oleifera leaf extract |
title_sort |
attenuation of lipopolysaccharide-induced neuroinflammatory events in bv-2 microglial cells by moringa oleifera leaf extract |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2019 |
url |
https://doi.org/10.4103/2221-1691.254604 https://doaj.org/article/48444daf58cb4208814ee82d328cce56 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 9, Iss 3, Pp 109-115 (2019) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2019;volume=9;issue=3;spage=109;epage=115;aulast=Sivaprakasam https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.254604 https://doaj.org/article/48444daf58cb4208814ee82d328cce56 |
op_doi |
https://doi.org/10.4103/2221-1691.254604 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
9 |
container_issue |
3 |
container_start_page |
109 |
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1766340276384694272 |