Volatile metabolomic signatures of rabies immunization in two mesocarnivore species.
Rabies is a zoonotic disease caused by infection with rabies virus, which circulates naturally in several wild carnivore and bat reservoirs in the United States (US). The most important reservoir in the US from an animal and public health perspective is the raccoon (Procyon lotor). To prevent the we...
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ftdoajarticles:oai:doaj.org/article:48305a1fd62e4d369c2b429fd5c378b7 2023-05-15T15:15:29+02:00 Volatile metabolomic signatures of rabies immunization in two mesocarnivore species. Bruce A Kimball Steven F Volker Doreen L Griffin Shylo R Johnson Amy T Gilbert 2019-12-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0007911 https://doaj.org/article/48305a1fd62e4d369c2b429fd5c378b7 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0007911 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007911 https://doaj.org/article/48305a1fd62e4d369c2b429fd5c378b7 PLoS Neglected Tropical Diseases, Vol 13, Iss 12, p e0007911 (2019) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2019 ftdoajarticles https://doi.org/10.1371/journal.pntd.0007911 2022-12-31T05:52:39Z Rabies is a zoonotic disease caused by infection with rabies virus, which circulates naturally in several wild carnivore and bat reservoirs in the United States (US). The most important reservoir in the US from an animal and public health perspective is the raccoon (Procyon lotor). To prevent the westward expansion of a significant raccoon rabies epizootic along the eastern seaboard, an operational control program implementing oral rabies vaccination (ORV) has existed in the US since the 1990s. Recently, two vaccine efficacy studies conducted with raccoons and striped skunks (Mephitis mephitis) provided the opportunity to determine if volatile fecal metabolites might be used to non-invasively monitor ORV programs and/or predict virus protection for these species. The volatile metabolome is a rich source of information that may significantly contribute to our understanding of disease and infection. Fecal samples were collected at multiple time points from raccoons and striped skunks subjected to oral treatment with rabies vaccine (or sham). Intramuscular challenge with a lethal dose of rabies virus was used to determine protection status at six (raccoons) and 11 (skunks) months post-vaccination. In addition to fecal samples, blood was collected at various time points to permit quantitative assessment of rabies antibody responses arising from immunization. Feces were analyzed by headspace gas chromatography with mass spectrometric detection and the chromatographic responses were grouped according to cluster analysis. Cluster scores were subjected to multivariate analyses of variance (MANOVA) to determine if fecal volatiles may hold a signal of immunization status. Multiple regression was then used to build models of the measured immune responses based on the metabolomic data. MANOVA results identified one cluster associated with protective status of skunks and one cluster associated with protective status of raccoons. Regression models demonstrated considerably greater success in predicting rabies antibody ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 13 12 e0007911 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Bruce A Kimball Steven F Volker Doreen L Griffin Shylo R Johnson Amy T Gilbert Volatile metabolomic signatures of rabies immunization in two mesocarnivore species. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Rabies is a zoonotic disease caused by infection with rabies virus, which circulates naturally in several wild carnivore and bat reservoirs in the United States (US). The most important reservoir in the US from an animal and public health perspective is the raccoon (Procyon lotor). To prevent the westward expansion of a significant raccoon rabies epizootic along the eastern seaboard, an operational control program implementing oral rabies vaccination (ORV) has existed in the US since the 1990s. Recently, two vaccine efficacy studies conducted with raccoons and striped skunks (Mephitis mephitis) provided the opportunity to determine if volatile fecal metabolites might be used to non-invasively monitor ORV programs and/or predict virus protection for these species. The volatile metabolome is a rich source of information that may significantly contribute to our understanding of disease and infection. Fecal samples were collected at multiple time points from raccoons and striped skunks subjected to oral treatment with rabies vaccine (or sham). Intramuscular challenge with a lethal dose of rabies virus was used to determine protection status at six (raccoons) and 11 (skunks) months post-vaccination. In addition to fecal samples, blood was collected at various time points to permit quantitative assessment of rabies antibody responses arising from immunization. Feces were analyzed by headspace gas chromatography with mass spectrometric detection and the chromatographic responses were grouped according to cluster analysis. Cluster scores were subjected to multivariate analyses of variance (MANOVA) to determine if fecal volatiles may hold a signal of immunization status. Multiple regression was then used to build models of the measured immune responses based on the metabolomic data. MANOVA results identified one cluster associated with protective status of skunks and one cluster associated with protective status of raccoons. Regression models demonstrated considerably greater success in predicting rabies antibody ... |
format |
Article in Journal/Newspaper |
author |
Bruce A Kimball Steven F Volker Doreen L Griffin Shylo R Johnson Amy T Gilbert |
author_facet |
Bruce A Kimball Steven F Volker Doreen L Griffin Shylo R Johnson Amy T Gilbert |
author_sort |
Bruce A Kimball |
title |
Volatile metabolomic signatures of rabies immunization in two mesocarnivore species. |
title_short |
Volatile metabolomic signatures of rabies immunization in two mesocarnivore species. |
title_full |
Volatile metabolomic signatures of rabies immunization in two mesocarnivore species. |
title_fullStr |
Volatile metabolomic signatures of rabies immunization in two mesocarnivore species. |
title_full_unstemmed |
Volatile metabolomic signatures of rabies immunization in two mesocarnivore species. |
title_sort |
volatile metabolomic signatures of rabies immunization in two mesocarnivore species. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2019 |
url |
https://doi.org/10.1371/journal.pntd.0007911 https://doaj.org/article/48305a1fd62e4d369c2b429fd5c378b7 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 13, Iss 12, p e0007911 (2019) |
op_relation |
https://doi.org/10.1371/journal.pntd.0007911 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007911 https://doaj.org/article/48305a1fd62e4d369c2b429fd5c378b7 |
op_doi |
https://doi.org/10.1371/journal.pntd.0007911 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
13 |
container_issue |
12 |
container_start_page |
e0007911 |
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