The genetic basis of salinity tolerance traits in Arctic charr ( Salvelinus alpinus )
Abstract Background The capacity to maintain internal ion homeostasis amidst changing conditions is particularly important for teleost fishes whose reproductive cycle is dependent upon movement from freshwater to seawater. Although the physiology of seawater osmoregulation in mitochondria-rich cells...
Published in: | BMC Genetics |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
BMC
2011
|
Subjects: | |
Online Access: | https://doi.org/10.1186/1471-2156-12-81 https://doaj.org/article/48150d25bcaa4da49c4d12651cad2883 |
id |
ftdoajarticles:oai:doaj.org/article:48150d25bcaa4da49c4d12651cad2883 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:48150d25bcaa4da49c4d12651cad2883 2023-05-15T14:30:00+02:00 The genetic basis of salinity tolerance traits in Arctic charr ( Salvelinus alpinus ) Glebe Brian Danzmann Roy G Norman Joseph D Ferguson Moira M 2011-09-01T00:00:00Z https://doi.org/10.1186/1471-2156-12-81 https://doaj.org/article/48150d25bcaa4da49c4d12651cad2883 EN eng BMC http://www.biomedcentral.com/1471-2156/12/81 https://doaj.org/toc/1471-2156 doi:10.1186/1471-2156-12-81 1471-2156 https://doaj.org/article/48150d25bcaa4da49c4d12651cad2883 BMC Genetics, Vol 12, Iss 1, p 81 (2011) Arctic charr salmonid fishes salinity tolerance Na + /K + -ATPase osmoregulation whole-genome duplications homeologies duplicated genes Genetics QH426-470 article 2011 ftdoajarticles https://doi.org/10.1186/1471-2156-12-81 2022-12-30T21:45:07Z Abstract Background The capacity to maintain internal ion homeostasis amidst changing conditions is particularly important for teleost fishes whose reproductive cycle is dependent upon movement from freshwater to seawater. Although the physiology of seawater osmoregulation in mitochondria-rich cells of fish gill epithelium is well understood, less is known about the underlying causes of inter- and intraspecific variation in salinity tolerance. We used a genome-scan approach in Arctic charr ( Salvelinus alpinus ) to map quantitative trait loci (QTL) correlated with variation in four salinity tolerance performance traits and six body size traits. Comparative genomics approaches allowed us to infer whether allelic variation at candidate gene loci (e.g., ATP1α1b, NKCC1, CFTR , and cldn10e ) could have underlain observed variation. Results Combined parental analyses yielded genome-wide significant QTL on linkage groups 8, 14 and 20 for salinity tolerance performance traits, and on 1, 19, 20 and 28 for body size traits. Several QTL exhibited chromosome-wide significance. Among the salinity tolerance performance QTL, trait co-localizations occurred on chromosomes 1, 4, 7, 18 and 20, while the greatest experimental variation was explained by QTL on chromosomes 20 (19.9%), 19 (14.2%), 4 (14.1%) and 12 (13.1%). Several QTL localized to linkage groups exhibiting homeologous affinities, and multiple QTL mapped to regions homologous with the positions of candidate gene loci in other teleosts. There was no gene × environment interaction among body size QTL and ambient salinity. Conclusions Variation in salinity tolerance capacity can be mapped to a subset of Arctic charr genomic regions that significantly influence performance in a seawater environment. The detection of QTL on linkage group 12 was consistent with the hypothesis that variation in salinity tolerance may be affected by allelic variation at the ATP1α1b locus. IGF2 may also affect salinity tolerance capacity as suggested by a genome-wide QTL on linkage group 19. ... Article in Journal/Newspaper Arctic charr Arctic Salvelinus alpinus Directory of Open Access Journals: DOAJ Articles Arctic BMC Genetics 12 1 81 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic charr salmonid fishes salinity tolerance Na + /K + -ATPase osmoregulation whole-genome duplications homeologies duplicated genes Genetics QH426-470 |
spellingShingle |
Arctic charr salmonid fishes salinity tolerance Na + /K + -ATPase osmoregulation whole-genome duplications homeologies duplicated genes Genetics QH426-470 Glebe Brian Danzmann Roy G Norman Joseph D Ferguson Moira M The genetic basis of salinity tolerance traits in Arctic charr ( Salvelinus alpinus ) |
topic_facet |
Arctic charr salmonid fishes salinity tolerance Na + /K + -ATPase osmoregulation whole-genome duplications homeologies duplicated genes Genetics QH426-470 |
description |
Abstract Background The capacity to maintain internal ion homeostasis amidst changing conditions is particularly important for teleost fishes whose reproductive cycle is dependent upon movement from freshwater to seawater. Although the physiology of seawater osmoregulation in mitochondria-rich cells of fish gill epithelium is well understood, less is known about the underlying causes of inter- and intraspecific variation in salinity tolerance. We used a genome-scan approach in Arctic charr ( Salvelinus alpinus ) to map quantitative trait loci (QTL) correlated with variation in four salinity tolerance performance traits and six body size traits. Comparative genomics approaches allowed us to infer whether allelic variation at candidate gene loci (e.g., ATP1α1b, NKCC1, CFTR , and cldn10e ) could have underlain observed variation. Results Combined parental analyses yielded genome-wide significant QTL on linkage groups 8, 14 and 20 for salinity tolerance performance traits, and on 1, 19, 20 and 28 for body size traits. Several QTL exhibited chromosome-wide significance. Among the salinity tolerance performance QTL, trait co-localizations occurred on chromosomes 1, 4, 7, 18 and 20, while the greatest experimental variation was explained by QTL on chromosomes 20 (19.9%), 19 (14.2%), 4 (14.1%) and 12 (13.1%). Several QTL localized to linkage groups exhibiting homeologous affinities, and multiple QTL mapped to regions homologous with the positions of candidate gene loci in other teleosts. There was no gene × environment interaction among body size QTL and ambient salinity. Conclusions Variation in salinity tolerance capacity can be mapped to a subset of Arctic charr genomic regions that significantly influence performance in a seawater environment. The detection of QTL on linkage group 12 was consistent with the hypothesis that variation in salinity tolerance may be affected by allelic variation at the ATP1α1b locus. IGF2 may also affect salinity tolerance capacity as suggested by a genome-wide QTL on linkage group 19. ... |
format |
Article in Journal/Newspaper |
author |
Glebe Brian Danzmann Roy G Norman Joseph D Ferguson Moira M |
author_facet |
Glebe Brian Danzmann Roy G Norman Joseph D Ferguson Moira M |
author_sort |
Glebe Brian |
title |
The genetic basis of salinity tolerance traits in Arctic charr ( Salvelinus alpinus ) |
title_short |
The genetic basis of salinity tolerance traits in Arctic charr ( Salvelinus alpinus ) |
title_full |
The genetic basis of salinity tolerance traits in Arctic charr ( Salvelinus alpinus ) |
title_fullStr |
The genetic basis of salinity tolerance traits in Arctic charr ( Salvelinus alpinus ) |
title_full_unstemmed |
The genetic basis of salinity tolerance traits in Arctic charr ( Salvelinus alpinus ) |
title_sort |
genetic basis of salinity tolerance traits in arctic charr ( salvelinus alpinus ) |
publisher |
BMC |
publishDate |
2011 |
url |
https://doi.org/10.1186/1471-2156-12-81 https://doaj.org/article/48150d25bcaa4da49c4d12651cad2883 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic charr Arctic Salvelinus alpinus |
genre_facet |
Arctic charr Arctic Salvelinus alpinus |
op_source |
BMC Genetics, Vol 12, Iss 1, p 81 (2011) |
op_relation |
http://www.biomedcentral.com/1471-2156/12/81 https://doaj.org/toc/1471-2156 doi:10.1186/1471-2156-12-81 1471-2156 https://doaj.org/article/48150d25bcaa4da49c4d12651cad2883 |
op_doi |
https://doi.org/10.1186/1471-2156-12-81 |
container_title |
BMC Genetics |
container_volume |
12 |
container_issue |
1 |
container_start_page |
81 |
_version_ |
1766303942512214016 |