Performance of a malaria microscopy image analysis slide reading device
Abstract Background Viewing Plasmodium in Romanovsky-stained blood has long been considered the gold standard for diagnosis and a cornerstone in management of the disease. This method however, requires a subjective evaluation by trained, experienced diagnosticians and establishing proficiency of dia...
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ftdoajarticles:oai:doaj.org/article:48053bfd950e4cb0beac8e2716d1fc6a 2023-05-15T15:16:11+02:00 Performance of a malaria microscopy image analysis slide reading device Prescott William R Jordan Robert G Grobusch Martin P Chinchilli Vernon M Kleinschmidt Immo Borovsky Joseph Plaskow Mark Torrez Miguel Mico Maximo Schwabe Christopher 2012-05-01T00:00:00Z https://doi.org/10.1186/1475-2875-11-155 https://doaj.org/article/48053bfd950e4cb0beac8e2716d1fc6a EN eng BMC http://www.malariajournal.com/content/11/1/155 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-11-155 1475-2875 https://doaj.org/article/48053bfd950e4cb0beac8e2716d1fc6a Malaria Journal, Vol 11, Iss 1, p 155 (2012) Malaria Diagnosis Image analysis Microscopy Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2012 ftdoajarticles https://doi.org/10.1186/1475-2875-11-155 2022-12-30T22:41:32Z Abstract Background Viewing Plasmodium in Romanovsky-stained blood has long been considered the gold standard for diagnosis and a cornerstone in management of the disease. This method however, requires a subjective evaluation by trained, experienced diagnosticians and establishing proficiency of diagnosis is fraught with many challenges. Reported here is an evaluation of a diagnostic system (a “device” consisting of a microscope, a scanner, and a computer algorithm) that evaluates scanned images of standard Giemsa-stained slides and reports species and parasitaemia. Methods The device was challenged with two independent tests: a 55 slide, expert slide reading test the composition of which has been published by the World Health Organization (“WHO55” test), and a second test in which slides were made from a sample of consenting subjects participating in a malaria incidence survey conducted in Equatorial Guinea (EGMIS test). These subjects’ blood was tested by malaria RDT as well as having the blood smear diagnosis unequivocally determined by a worldwide panel of a minimum of six reference microscopists. Only slides with unequivocal microscopic diagnoses were used for the device challenge, n = 119. Results On the WHO55 test, the device scored a “Level 4” using the WHO published grading scheme. Broken down by more traditional analysis parameters this result was translated to 89% and 70% sensitivity and specificity, respectively. Species were correctly identified in 61% of the slides and the quantification of parasites fell within acceptable range of the validated parasitaemia in 10% of the cases. On the EGMIS test it scored 100% and 94% sensitivity/specificity, with 64% of the species correct and 45% of the parasitaemia within an acceptable range. A pooled analysis of the 174 slides used for both tests resulted in an overall 92% sensitivity and 90% specificity with 61% species and 19% quantifications correct. Conclusions In its current manifestation, the device performs at a level comparable to that of many human ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 11 1 |
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op_collection_id |
ftdoajarticles |
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English |
topic |
Malaria Diagnosis Image analysis Microscopy Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Malaria Diagnosis Image analysis Microscopy Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Prescott William R Jordan Robert G Grobusch Martin P Chinchilli Vernon M Kleinschmidt Immo Borovsky Joseph Plaskow Mark Torrez Miguel Mico Maximo Schwabe Christopher Performance of a malaria microscopy image analysis slide reading device |
topic_facet |
Malaria Diagnosis Image analysis Microscopy Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Viewing Plasmodium in Romanovsky-stained blood has long been considered the gold standard for diagnosis and a cornerstone in management of the disease. This method however, requires a subjective evaluation by trained, experienced diagnosticians and establishing proficiency of diagnosis is fraught with many challenges. Reported here is an evaluation of a diagnostic system (a “device” consisting of a microscope, a scanner, and a computer algorithm) that evaluates scanned images of standard Giemsa-stained slides and reports species and parasitaemia. Methods The device was challenged with two independent tests: a 55 slide, expert slide reading test the composition of which has been published by the World Health Organization (“WHO55” test), and a second test in which slides were made from a sample of consenting subjects participating in a malaria incidence survey conducted in Equatorial Guinea (EGMIS test). These subjects’ blood was tested by malaria RDT as well as having the blood smear diagnosis unequivocally determined by a worldwide panel of a minimum of six reference microscopists. Only slides with unequivocal microscopic diagnoses were used for the device challenge, n = 119. Results On the WHO55 test, the device scored a “Level 4” using the WHO published grading scheme. Broken down by more traditional analysis parameters this result was translated to 89% and 70% sensitivity and specificity, respectively. Species were correctly identified in 61% of the slides and the quantification of parasites fell within acceptable range of the validated parasitaemia in 10% of the cases. On the EGMIS test it scored 100% and 94% sensitivity/specificity, with 64% of the species correct and 45% of the parasitaemia within an acceptable range. A pooled analysis of the 174 slides used for both tests resulted in an overall 92% sensitivity and 90% specificity with 61% species and 19% quantifications correct. Conclusions In its current manifestation, the device performs at a level comparable to that of many human ... |
format |
Article in Journal/Newspaper |
author |
Prescott William R Jordan Robert G Grobusch Martin P Chinchilli Vernon M Kleinschmidt Immo Borovsky Joseph Plaskow Mark Torrez Miguel Mico Maximo Schwabe Christopher |
author_facet |
Prescott William R Jordan Robert G Grobusch Martin P Chinchilli Vernon M Kleinschmidt Immo Borovsky Joseph Plaskow Mark Torrez Miguel Mico Maximo Schwabe Christopher |
author_sort |
Prescott William R |
title |
Performance of a malaria microscopy image analysis slide reading device |
title_short |
Performance of a malaria microscopy image analysis slide reading device |
title_full |
Performance of a malaria microscopy image analysis slide reading device |
title_fullStr |
Performance of a malaria microscopy image analysis slide reading device |
title_full_unstemmed |
Performance of a malaria microscopy image analysis slide reading device |
title_sort |
performance of a malaria microscopy image analysis slide reading device |
publisher |
BMC |
publishDate |
2012 |
url |
https://doi.org/10.1186/1475-2875-11-155 https://doaj.org/article/48053bfd950e4cb0beac8e2716d1fc6a |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 11, Iss 1, p 155 (2012) |
op_relation |
http://www.malariajournal.com/content/11/1/155 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-11-155 1475-2875 https://doaj.org/article/48053bfd950e4cb0beac8e2716d1fc6a |
op_doi |
https://doi.org/10.1186/1475-2875-11-155 |
container_title |
Malaria Journal |
container_volume |
11 |
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1 |
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1766346474388455424 |