Heterophilic antibodies in sera from individuals without loxoscelism cross-react with phospholipase D from the venom of Loxosceles and Sicarius spiders
Abstract Background Loxoscelism is a severe human envenomation caused by Loxosceles spider venom. To the best of our knowledge, no study has evaluated the presence of antibodies against Loxosceles venom in loxoscelism patients without treatment with antivenom immunotherapy. We perform a comparative...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:4800b278c38b42e5a9904a6583fb602a 2023-05-15T15:16:27+02:00 Heterophilic antibodies in sera from individuals without loxoscelism cross-react with phospholipase D from the venom of Loxosceles and Sicarius spiders Tomás Arán-Sekul José M. Rojas Mario Subiabre Victoria Cruz William Cortés Luis Osorio Jorge González Jorge E. Araya Alejandro Catalán 2018-07-01T00:00:00Z https://doi.org/10.1186/s40409-018-0155-x https://doaj.org/article/4800b278c38b42e5a9904a6583fb602a EN eng SciELO http://link.springer.com/article/10.1186/s40409-018-0155-x https://doaj.org/toc/1678-9199 doi:10.1186/s40409-018-0155-x 1678-9199 https://doaj.org/article/4800b278c38b42e5a9904a6583fb602a Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 24, Iss 1, Pp 1-14 (2018) Loxosceles laeta Heterophilic antibodies Natural antibodies Sicarius Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2018 ftdoajarticles https://doi.org/10.1186/s40409-018-0155-x 2022-12-31T16:03:18Z Abstract Background Loxoscelism is a severe human envenomation caused by Loxosceles spider venom. To the best of our knowledge, no study has evaluated the presence of antibodies against Loxosceles venom in loxoscelism patients without treatment with antivenom immunotherapy. We perform a comparative analysis for the presence of antibodies capable of recognizing Loxosceles venom in a group of patients diagnosed with loxoscelism and in a group of people without loxoscelism. Methods The detection of L. laeta venom, Sicarius venom and recombinant phospholipases D from Loxosceles (PLDs) in sera from people with loxoscelism (Group 1) and from healthy people with no history of loxoscelism (Group 2) was evaluated using immuno-dot blot, indirect ELISA, and Western blot. Results We found naturally heterophilic antibodies (IgG-type) in people without contact with Loxosceles spiders or any clinical history of loxoscelism. Either serum pools or single sera from Group 1 and Group 2 analyzed by dot blot tested positive for L. laeta venom. Indirect ELISA for venom recognition showed titles of 1:320 for Group 1 sera and 1:160 for Group 2 sera. Total IgG quantification showed no difference in sera from both groups. Pooled sera and purified IgG from sera of both groups revealed venom proteins between 25 and 32 kDa and the recombinant phospholipase D isoform 1 (rLlPLD1), specifically. Moreover, heterophile antibodies cross-react with PLDs from other Loxosceles species and the venom of Sicarius spider. Conclusions People without contact with the spider venom produced heterophilic antibodies capable of generating a cross-reaction against the venom of L. laeta and Sicarius spiders. Their presence and possible interference should be considered in the development of immunoassays for Loxosceles venom detection. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 24 1 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
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Loxosceles laeta Heterophilic antibodies Natural antibodies Sicarius Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
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Loxosceles laeta Heterophilic antibodies Natural antibodies Sicarius Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Tomás Arán-Sekul José M. Rojas Mario Subiabre Victoria Cruz William Cortés Luis Osorio Jorge González Jorge E. Araya Alejandro Catalán Heterophilic antibodies in sera from individuals without loxoscelism cross-react with phospholipase D from the venom of Loxosceles and Sicarius spiders |
topic_facet |
Loxosceles laeta Heterophilic antibodies Natural antibodies Sicarius Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Abstract Background Loxoscelism is a severe human envenomation caused by Loxosceles spider venom. To the best of our knowledge, no study has evaluated the presence of antibodies against Loxosceles venom in loxoscelism patients without treatment with antivenom immunotherapy. We perform a comparative analysis for the presence of antibodies capable of recognizing Loxosceles venom in a group of patients diagnosed with loxoscelism and in a group of people without loxoscelism. Methods The detection of L. laeta venom, Sicarius venom and recombinant phospholipases D from Loxosceles (PLDs) in sera from people with loxoscelism (Group 1) and from healthy people with no history of loxoscelism (Group 2) was evaluated using immuno-dot blot, indirect ELISA, and Western blot. Results We found naturally heterophilic antibodies (IgG-type) in people without contact with Loxosceles spiders or any clinical history of loxoscelism. Either serum pools or single sera from Group 1 and Group 2 analyzed by dot blot tested positive for L. laeta venom. Indirect ELISA for venom recognition showed titles of 1:320 for Group 1 sera and 1:160 for Group 2 sera. Total IgG quantification showed no difference in sera from both groups. Pooled sera and purified IgG from sera of both groups revealed venom proteins between 25 and 32 kDa and the recombinant phospholipase D isoform 1 (rLlPLD1), specifically. Moreover, heterophile antibodies cross-react with PLDs from other Loxosceles species and the venom of Sicarius spider. Conclusions People without contact with the spider venom produced heterophilic antibodies capable of generating a cross-reaction against the venom of L. laeta and Sicarius spiders. Their presence and possible interference should be considered in the development of immunoassays for Loxosceles venom detection. |
format |
Article in Journal/Newspaper |
author |
Tomás Arán-Sekul José M. Rojas Mario Subiabre Victoria Cruz William Cortés Luis Osorio Jorge González Jorge E. Araya Alejandro Catalán |
author_facet |
Tomás Arán-Sekul José M. Rojas Mario Subiabre Victoria Cruz William Cortés Luis Osorio Jorge González Jorge E. Araya Alejandro Catalán |
author_sort |
Tomás Arán-Sekul |
title |
Heterophilic antibodies in sera from individuals without loxoscelism cross-react with phospholipase D from the venom of Loxosceles and Sicarius spiders |
title_short |
Heterophilic antibodies in sera from individuals without loxoscelism cross-react with phospholipase D from the venom of Loxosceles and Sicarius spiders |
title_full |
Heterophilic antibodies in sera from individuals without loxoscelism cross-react with phospholipase D from the venom of Loxosceles and Sicarius spiders |
title_fullStr |
Heterophilic antibodies in sera from individuals without loxoscelism cross-react with phospholipase D from the venom of Loxosceles and Sicarius spiders |
title_full_unstemmed |
Heterophilic antibodies in sera from individuals without loxoscelism cross-react with phospholipase D from the venom of Loxosceles and Sicarius spiders |
title_sort |
heterophilic antibodies in sera from individuals without loxoscelism cross-react with phospholipase d from the venom of loxosceles and sicarius spiders |
publisher |
SciELO |
publishDate |
2018 |
url |
https://doi.org/10.1186/s40409-018-0155-x https://doaj.org/article/4800b278c38b42e5a9904a6583fb602a |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 24, Iss 1, Pp 1-14 (2018) |
op_relation |
http://link.springer.com/article/10.1186/s40409-018-0155-x https://doaj.org/toc/1678-9199 doi:10.1186/s40409-018-0155-x 1678-9199 https://doaj.org/article/4800b278c38b42e5a9904a6583fb602a |
op_doi |
https://doi.org/10.1186/s40409-018-0155-x |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
24 |
container_issue |
1 |
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1766346754548039680 |