Quantitative lipidomic analysis of Ascaris suum.
Ascaris is a soil-transmitted nematode that causes ascariasis, a neglected tropical disease affecting predominantly children and adolescents in the tropics and subtropics. Approximately 0.8 billion people are affected worldwide, equating to 0.86 million disability-adjusted life-years (DALYs). Explor...
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ftdoajarticles:oai:doaj.org/article:47b933b7106840f09736e264a09a79bc 2023-05-15T15:13:24+02:00 Quantitative lipidomic analysis of Ascaris suum. Tao Wang Shuai Nie Guangxu Ma Johnny Vlaminck Peter Geldhof Nicholas A Williamson Gavin E Reid Robin B Gasser 2020-12-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008848 https://doaj.org/article/47b933b7106840f09736e264a09a79bc EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008848 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008848 https://doaj.org/article/47b933b7106840f09736e264a09a79bc PLoS Neglected Tropical Diseases, Vol 14, Iss 12, p e0008848 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008848 2022-12-31T07:16:57Z Ascaris is a soil-transmitted nematode that causes ascariasis, a neglected tropical disease affecting predominantly children and adolescents in the tropics and subtropics. Approximately 0.8 billion people are affected worldwide, equating to 0.86 million disability-adjusted life-years (DALYs). Exploring the molecular biology of Ascaris is important to gain a better understanding of the host-parasite interactions and disease processes, and supports the development of novel interventions. Although advances have been made in the genomics, transcriptomics and proteomics of Ascaris, its lipidome has received very limited attention. Lipidomics is an important sub-discipline of systems biology, focused on exploring lipids profiles in tissues and cells, and elucidating their biological and metabolic roles. Here, we characterised the lipidomes of key developmental stages and organ systems of Ascaris of porcine origin via high throughput LC-MS/MS. In total, > 500 lipid species belonging to 18 lipid classes within three lipid categories were identified and quantified-in precise molar amounts in relation to the dry weight of worm material-in different developmental stages/sexes and organ systems. The results showed substantial differences in the composition and abundance of lipids with key roles in cellular processes and functions (e.g. energy storage regulation and membrane structure) among distinct stages and among organ systems, likely reflecting differing demands for lipids, depending on stage of growth and development as well as the need to adapt to constantly changing environments within and outside of the host animal. This work provides the first step toward understanding the biology of lipids in Ascaris, with possibilities to work toward designing new interventions against ascariasis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 14 12 e0008848 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Tao Wang Shuai Nie Guangxu Ma Johnny Vlaminck Peter Geldhof Nicholas A Williamson Gavin E Reid Robin B Gasser Quantitative lipidomic analysis of Ascaris suum. |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Ascaris is a soil-transmitted nematode that causes ascariasis, a neglected tropical disease affecting predominantly children and adolescents in the tropics and subtropics. Approximately 0.8 billion people are affected worldwide, equating to 0.86 million disability-adjusted life-years (DALYs). Exploring the molecular biology of Ascaris is important to gain a better understanding of the host-parasite interactions and disease processes, and supports the development of novel interventions. Although advances have been made in the genomics, transcriptomics and proteomics of Ascaris, its lipidome has received very limited attention. Lipidomics is an important sub-discipline of systems biology, focused on exploring lipids profiles in tissues and cells, and elucidating their biological and metabolic roles. Here, we characterised the lipidomes of key developmental stages and organ systems of Ascaris of porcine origin via high throughput LC-MS/MS. In total, > 500 lipid species belonging to 18 lipid classes within three lipid categories were identified and quantified-in precise molar amounts in relation to the dry weight of worm material-in different developmental stages/sexes and organ systems. The results showed substantial differences in the composition and abundance of lipids with key roles in cellular processes and functions (e.g. energy storage regulation and membrane structure) among distinct stages and among organ systems, likely reflecting differing demands for lipids, depending on stage of growth and development as well as the need to adapt to constantly changing environments within and outside of the host animal. This work provides the first step toward understanding the biology of lipids in Ascaris, with possibilities to work toward designing new interventions against ascariasis. |
format |
Article in Journal/Newspaper |
author |
Tao Wang Shuai Nie Guangxu Ma Johnny Vlaminck Peter Geldhof Nicholas A Williamson Gavin E Reid Robin B Gasser |
author_facet |
Tao Wang Shuai Nie Guangxu Ma Johnny Vlaminck Peter Geldhof Nicholas A Williamson Gavin E Reid Robin B Gasser |
author_sort |
Tao Wang |
title |
Quantitative lipidomic analysis of Ascaris suum. |
title_short |
Quantitative lipidomic analysis of Ascaris suum. |
title_full |
Quantitative lipidomic analysis of Ascaris suum. |
title_fullStr |
Quantitative lipidomic analysis of Ascaris suum. |
title_full_unstemmed |
Quantitative lipidomic analysis of Ascaris suum. |
title_sort |
quantitative lipidomic analysis of ascaris suum. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0008848 https://doaj.org/article/47b933b7106840f09736e264a09a79bc |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 12, p e0008848 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008848 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008848 https://doaj.org/article/47b933b7106840f09736e264a09a79bc |
op_doi |
https://doi.org/10.1371/journal.pntd.0008848 |
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PLOS Neglected Tropical Diseases |
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14 |
container_issue |
12 |
container_start_page |
e0008848 |
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1766343954369871872 |