Functional activity of monocytes and macrophages in HTLV-1 infected subjects.
The Human T lymphotropic virus type-1 (HTLV-1) infects predominantly T cells, inducing proliferation and lymphocyte activation. Additionally, HTLV-1 infected subjects are more susceptible to other infections caused by other intracellular agents. Monocytes/macrophages are important cells in the defen...
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ftdoajarticles:oai:doaj.org/article:4798fe15f1c84242abb0a14189024b71 2023-05-15T15:14:15+02:00 Functional activity of monocytes and macrophages in HTLV-1 infected subjects. Camila F Amorim Anselmo S Souza Angela G Diniz Natália B Carvalho Silvane B Santos Edgar M Carvalho 2014-12-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0003399 https://doaj.org/article/4798fe15f1c84242abb0a14189024b71 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4270688?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003399 https://doaj.org/article/4798fe15f1c84242abb0a14189024b71 PLoS Neglected Tropical Diseases, Vol 8, Iss 12, p e3399 (2014) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2014 ftdoajarticles https://doi.org/10.1371/journal.pntd.0003399 2022-12-31T00:59:32Z The Human T lymphotropic virus type-1 (HTLV-1) infects predominantly T cells, inducing proliferation and lymphocyte activation. Additionally, HTLV-1 infected subjects are more susceptible to other infections caused by other intracellular agents. Monocytes/macrophages are important cells in the defense against intracellular pathogens. Our aims were to determine the frequency of monocytes subsets, expression of co-stimulatory molecules in these cells and to evaluate microbicidal ability and cytokine and chemokine production by macrophages from HTLV-1 infected subjects. Participants were 23 HTLV-1 carriers (HC), 22 HAM/TSP patients and 22 healthy subjects (HS) not infected with HTLV-1. The frequencies of monocyte subsets and expression of co-stimulatory molecules were determined by flow cytometry. Macrophages were infected with L. braziliensis or stimulated with LPS. Microbicidal activity of macrophages was determined by optic microscopy. Cytokines/chemokines from macrophage supernatants were measured by ELISA. HAM/TSP patients showed an increase frequency of intermediate monocytes, but expression of co-stimulatory molecules was similar between the groups. Macrophages from HTLV-1 infected individuals were infected with L. braziliensis at the same ratio than macrophages from HS, and all the groups had the same ability to kill Leishmania parasites. However, macrophages from HTLV-1 infected subjects produced more CXCL9 and CCL5, and less IL-10 than cells from HS. While there was no correlation between IFN-γ and cytokine/chemokine production by macrophages, there was a correlation between proviral load and TNF and CXCL10. These data showed a dissociation between the inflammatory response and microbicidal ability of macrophages from HTLV-1 infected subjects. While macrophages ability to kill an intracellular pathogen did not differ among HTLV-1 infected subjects, these cells secreted high amount of chemokines even in unstimulated cultures. Moreover the increasing inflammatory activity of macrophages was similar in ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 8 12 e3399 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Camila F Amorim Anselmo S Souza Angela G Diniz Natália B Carvalho Silvane B Santos Edgar M Carvalho Functional activity of monocytes and macrophages in HTLV-1 infected subjects. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The Human T lymphotropic virus type-1 (HTLV-1) infects predominantly T cells, inducing proliferation and lymphocyte activation. Additionally, HTLV-1 infected subjects are more susceptible to other infections caused by other intracellular agents. Monocytes/macrophages are important cells in the defense against intracellular pathogens. Our aims were to determine the frequency of monocytes subsets, expression of co-stimulatory molecules in these cells and to evaluate microbicidal ability and cytokine and chemokine production by macrophages from HTLV-1 infected subjects. Participants were 23 HTLV-1 carriers (HC), 22 HAM/TSP patients and 22 healthy subjects (HS) not infected with HTLV-1. The frequencies of monocyte subsets and expression of co-stimulatory molecules were determined by flow cytometry. Macrophages were infected with L. braziliensis or stimulated with LPS. Microbicidal activity of macrophages was determined by optic microscopy. Cytokines/chemokines from macrophage supernatants were measured by ELISA. HAM/TSP patients showed an increase frequency of intermediate monocytes, but expression of co-stimulatory molecules was similar between the groups. Macrophages from HTLV-1 infected individuals were infected with L. braziliensis at the same ratio than macrophages from HS, and all the groups had the same ability to kill Leishmania parasites. However, macrophages from HTLV-1 infected subjects produced more CXCL9 and CCL5, and less IL-10 than cells from HS. While there was no correlation between IFN-γ and cytokine/chemokine production by macrophages, there was a correlation between proviral load and TNF and CXCL10. These data showed a dissociation between the inflammatory response and microbicidal ability of macrophages from HTLV-1 infected subjects. While macrophages ability to kill an intracellular pathogen did not differ among HTLV-1 infected subjects, these cells secreted high amount of chemokines even in unstimulated cultures. Moreover the increasing inflammatory activity of macrophages was similar in ... |
format |
Article in Journal/Newspaper |
author |
Camila F Amorim Anselmo S Souza Angela G Diniz Natália B Carvalho Silvane B Santos Edgar M Carvalho |
author_facet |
Camila F Amorim Anselmo S Souza Angela G Diniz Natália B Carvalho Silvane B Santos Edgar M Carvalho |
author_sort |
Camila F Amorim |
title |
Functional activity of monocytes and macrophages in HTLV-1 infected subjects. |
title_short |
Functional activity of monocytes and macrophages in HTLV-1 infected subjects. |
title_full |
Functional activity of monocytes and macrophages in HTLV-1 infected subjects. |
title_fullStr |
Functional activity of monocytes and macrophages in HTLV-1 infected subjects. |
title_full_unstemmed |
Functional activity of monocytes and macrophages in HTLV-1 infected subjects. |
title_sort |
functional activity of monocytes and macrophages in htlv-1 infected subjects. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2014 |
url |
https://doi.org/10.1371/journal.pntd.0003399 https://doaj.org/article/4798fe15f1c84242abb0a14189024b71 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 8, Iss 12, p e3399 (2014) |
op_relation |
http://europepmc.org/articles/PMC4270688?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003399 https://doaj.org/article/4798fe15f1c84242abb0a14189024b71 |
op_doi |
https://doi.org/10.1371/journal.pntd.0003399 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
8 |
container_issue |
12 |
container_start_page |
e3399 |
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1766344724681064448 |