The current state of Pinus sylvestris L. gene pool in Karelia
Three native populations of P. sylvestris, ssp. lapponica (Alakurtti, Gridino, Pjaozero) and six native populations of P. s., ssp. sylvestris (Voinitsa, Maslozero, Vodlozero, Zaonezshye, Kivach, Sortavala) along with one artificial population – Petrozavodsk seed orchard have been studied using four...
Published in: | Сибирский лесной журнал |
---|---|
Main Authors: | , |
Format: | Article in Journal/Newspaper |
Language: | English Russian |
Published: |
Russian Academy of Sciences, Siberian Branch Publishing House
2016
|
Subjects: | |
Online Access: | https://doi.org/10.15372/SJFS20160504 https://doaj.org/article/478c9aa64045453e8663eb6d27476fd8 |
id |
ftdoajarticles:oai:doaj.org/article:478c9aa64045453e8663eb6d27476fd8 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:478c9aa64045453e8663eb6d27476fd8 2023-05-15T17:01:28+02:00 The current state of Pinus sylvestris L. gene pool in Karelia А. А. Ilyinov B. V. Raevsky 2016-10-01T00:00:00Z https://doi.org/10.15372/SJFS20160504 https://doaj.org/article/478c9aa64045453e8663eb6d27476fd8 EN RU eng rus Russian Academy of Sciences, Siberian Branch Publishing House http://xn--80abmehbaibgnewcmzjeef0c.xn--p1ai/upload/iblock/e62/e626f8a5e370bc733005c262fadd352f.pdf https://doaj.org/toc/2311-1410 https://doaj.org/toc/2312-2099 doi:10.15372/SJFS20160504 2311-1410 2312-2099 https://doaj.org/article/478c9aa64045453e8663eb6d27476fd8 Сибирский лесной журнал, Vol 3, Iss 5, Pp 45-54 (2016) Pinus sylvestris native populations genetic structure PCR microsatellites genetic diversity Forestry SD1-669.5 article 2016 ftdoajarticles https://doi.org/10.15372/SJFS20160504 2022-12-31T10:19:25Z Three native populations of P. sylvestris, ssp. lapponica (Alakurtti, Gridino, Pjaozero) and six native populations of P. s., ssp. sylvestris (Voinitsa, Maslozero, Vodlozero, Zaonezshye, Kivach, Sortavala) along with one artificial population – Petrozavodsk seed orchard have been studied using four nuclear microsatellite primers (Spac11.8, Spac12.5, PtTX2123, PtTX2146) to find out the peculiarities of Scotch pine intraspecific diversity in Karelia. In total, 66 alleles were found. All four loci turned out to be polymorphic in all populations. The observed heterozigocity level of all studied Karelian populations was lower than expected, which is evidence of heterozygotes deficiency in Karelian pine populations. Overall, the investigated P. sylvestris populations can be described as having a high level of the genetic variability especially compared to results obtained earlier by isozyme analyses. The Petrozavodsk seed orchard ought to be mentioned as having one of the highest levels of the genetic variability, which gives evidences in favor of good state regarding representativeness of Scotch pine gene pool here. No substantial differences in genetic structure and diversity levels have been found for ss. lapponica populations vs. ss. sylvestris ones. AMOVA analysis showed that despite the significant differences between pine populations, considering both the allele composition and the genetic diversity, the major part of the variety (90 %) was allocated inside group. The investigated populations were subdivided into two clusters using UPGMA technique characterized by substantial Nei distance (DN = 0.273). The former one included the majority of Karelian pine populations (DN = 0.030–0.082) along with the Alakurtti population from Murmansk region. The latter cluster consisted ofthe most isolated «Kivach» and «Zaonezshye» populations, which had the lowest levels of genetic diversity, and the «Petrozavodsk seed orchard». Article in Journal/Newspaper karelian Directory of Open Access Journals: DOAJ Articles Alakurtti ENVELOPE(30.349,30.349,66.967,66.967) Gridino ENVELOPE(34.675,34.675,65.923,65.923) Murmansk Сибирский лесной журнал 5 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English Russian |
topic |
Pinus sylvestris native populations genetic structure PCR microsatellites genetic diversity Forestry SD1-669.5 |
spellingShingle |
Pinus sylvestris native populations genetic structure PCR microsatellites genetic diversity Forestry SD1-669.5 А. А. Ilyinov B. V. Raevsky The current state of Pinus sylvestris L. gene pool in Karelia |
topic_facet |
Pinus sylvestris native populations genetic structure PCR microsatellites genetic diversity Forestry SD1-669.5 |
description |
Three native populations of P. sylvestris, ssp. lapponica (Alakurtti, Gridino, Pjaozero) and six native populations of P. s., ssp. sylvestris (Voinitsa, Maslozero, Vodlozero, Zaonezshye, Kivach, Sortavala) along with one artificial population – Petrozavodsk seed orchard have been studied using four nuclear microsatellite primers (Spac11.8, Spac12.5, PtTX2123, PtTX2146) to find out the peculiarities of Scotch pine intraspecific diversity in Karelia. In total, 66 alleles were found. All four loci turned out to be polymorphic in all populations. The observed heterozigocity level of all studied Karelian populations was lower than expected, which is evidence of heterozygotes deficiency in Karelian pine populations. Overall, the investigated P. sylvestris populations can be described as having a high level of the genetic variability especially compared to results obtained earlier by isozyme analyses. The Petrozavodsk seed orchard ought to be mentioned as having one of the highest levels of the genetic variability, which gives evidences in favor of good state regarding representativeness of Scotch pine gene pool here. No substantial differences in genetic structure and diversity levels have been found for ss. lapponica populations vs. ss. sylvestris ones. AMOVA analysis showed that despite the significant differences between pine populations, considering both the allele composition and the genetic diversity, the major part of the variety (90 %) was allocated inside group. The investigated populations were subdivided into two clusters using UPGMA technique characterized by substantial Nei distance (DN = 0.273). The former one included the majority of Karelian pine populations (DN = 0.030–0.082) along with the Alakurtti population from Murmansk region. The latter cluster consisted ofthe most isolated «Kivach» and «Zaonezshye» populations, which had the lowest levels of genetic diversity, and the «Petrozavodsk seed orchard». |
format |
Article in Journal/Newspaper |
author |
А. А. Ilyinov B. V. Raevsky |
author_facet |
А. А. Ilyinov B. V. Raevsky |
author_sort |
А. А. Ilyinov |
title |
The current state of Pinus sylvestris L. gene pool in Karelia |
title_short |
The current state of Pinus sylvestris L. gene pool in Karelia |
title_full |
The current state of Pinus sylvestris L. gene pool in Karelia |
title_fullStr |
The current state of Pinus sylvestris L. gene pool in Karelia |
title_full_unstemmed |
The current state of Pinus sylvestris L. gene pool in Karelia |
title_sort |
current state of pinus sylvestris l. gene pool in karelia |
publisher |
Russian Academy of Sciences, Siberian Branch Publishing House |
publishDate |
2016 |
url |
https://doi.org/10.15372/SJFS20160504 https://doaj.org/article/478c9aa64045453e8663eb6d27476fd8 |
long_lat |
ENVELOPE(30.349,30.349,66.967,66.967) ENVELOPE(34.675,34.675,65.923,65.923) |
geographic |
Alakurtti Gridino Murmansk |
geographic_facet |
Alakurtti Gridino Murmansk |
genre |
karelian |
genre_facet |
karelian |
op_source |
Сибирский лесной журнал, Vol 3, Iss 5, Pp 45-54 (2016) |
op_relation |
http://xn--80abmehbaibgnewcmzjeef0c.xn--p1ai/upload/iblock/e62/e626f8a5e370bc733005c262fadd352f.pdf https://doaj.org/toc/2311-1410 https://doaj.org/toc/2312-2099 doi:10.15372/SJFS20160504 2311-1410 2312-2099 https://doaj.org/article/478c9aa64045453e8663eb6d27476fd8 |
op_doi |
https://doi.org/10.15372/SJFS20160504 |
container_title |
Сибирский лесной журнал |
container_issue |
5 |
_version_ |
1766054569589080064 |