Serological and Molecular Investigation of Batai Virus Infections in Ruminants from the State of Saxony-Anhalt, Germany, 2018
Arthropod-borne Batai virus (BATV) is an Orthobunyavirus widely distributed throughout European livestock and has, in the past, been linked to febrile diseases in humans. In Germany, BATV was found in mosquitoes and in one captive harbor seal, and antibodies were recently detected in various ruminan...
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ftdoajarticles:oai:doaj.org/article:4786d8f4a1344a0db89627d78856eed7 2024-01-14T10:07:27+01:00 Serological and Molecular Investigation of Batai Virus Infections in Ruminants from the State of Saxony-Anhalt, Germany, 2018 Nicole Cichon Martin Eiden Jana Schulz Anne Günther Patrick Wysocki Cora M. Holicki Joachim Borgwardt Wolfgang Gaede Martin H. Groschup Ute Ziegler 2021-02-01T00:00:00Z https://doi.org/10.3390/v13030370 https://doaj.org/article/4786d8f4a1344a0db89627d78856eed7 EN eng MDPI AG https://www.mdpi.com/1999-4915/13/3/370 https://doaj.org/toc/1999-4915 doi:10.3390/v13030370 1999-4915 https://doaj.org/article/4786d8f4a1344a0db89627d78856eed7 Viruses, Vol 13, Iss 3, p 370 (2021) Batai virus ELISA seroprevalence ruminants Germany Microbiology QR1-502 article 2021 ftdoajarticles https://doi.org/10.3390/v13030370 2023-12-17T01:45:01Z Arthropod-borne Batai virus (BATV) is an Orthobunyavirus widely distributed throughout European livestock and has, in the past, been linked to febrile diseases in humans. In Germany, BATV was found in mosquitoes and in one captive harbor seal, and antibodies were recently detected in various ruminant species. We have, therefore, conducted a follow-up study in ruminants from Saxony-Anhalt, the most affected region in Eastern Germany. A total of 325 blood samples from apparently healthy sheep, goats, and cattle were tested using a BATV-specific qRT-PCR and SNT. Even though viral RNA was not detected, the presence of antibodies was confirmed in the sera of all three species: sheep (16.5%), goats (18.3%), and cattle (41.4%). Sera were further analyzed by a glycoprotein Gc-based indirect ELISA to evaluate Gc-derived antibodies as a basis for a new serological test for BATV infections. Interestingly, the presence of neutralizing antibodies was not directly linked to the presence of BATV Gc antibodies. Overall, our results illustrate the high frequency of BATV infections in ruminants in Eastern Germany. Article in Journal/Newspaper harbor seal Directory of Open Access Journals: DOAJ Articles Viruses 13 3 370 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Batai virus ELISA seroprevalence ruminants Germany Microbiology QR1-502 |
spellingShingle |
Batai virus ELISA seroprevalence ruminants Germany Microbiology QR1-502 Nicole Cichon Martin Eiden Jana Schulz Anne Günther Patrick Wysocki Cora M. Holicki Joachim Borgwardt Wolfgang Gaede Martin H. Groschup Ute Ziegler Serological and Molecular Investigation of Batai Virus Infections in Ruminants from the State of Saxony-Anhalt, Germany, 2018 |
topic_facet |
Batai virus ELISA seroprevalence ruminants Germany Microbiology QR1-502 |
description |
Arthropod-borne Batai virus (BATV) is an Orthobunyavirus widely distributed throughout European livestock and has, in the past, been linked to febrile diseases in humans. In Germany, BATV was found in mosquitoes and in one captive harbor seal, and antibodies were recently detected in various ruminant species. We have, therefore, conducted a follow-up study in ruminants from Saxony-Anhalt, the most affected region in Eastern Germany. A total of 325 blood samples from apparently healthy sheep, goats, and cattle were tested using a BATV-specific qRT-PCR and SNT. Even though viral RNA was not detected, the presence of antibodies was confirmed in the sera of all three species: sheep (16.5%), goats (18.3%), and cattle (41.4%). Sera were further analyzed by a glycoprotein Gc-based indirect ELISA to evaluate Gc-derived antibodies as a basis for a new serological test for BATV infections. Interestingly, the presence of neutralizing antibodies was not directly linked to the presence of BATV Gc antibodies. Overall, our results illustrate the high frequency of BATV infections in ruminants in Eastern Germany. |
format |
Article in Journal/Newspaper |
author |
Nicole Cichon Martin Eiden Jana Schulz Anne Günther Patrick Wysocki Cora M. Holicki Joachim Borgwardt Wolfgang Gaede Martin H. Groschup Ute Ziegler |
author_facet |
Nicole Cichon Martin Eiden Jana Schulz Anne Günther Patrick Wysocki Cora M. Holicki Joachim Borgwardt Wolfgang Gaede Martin H. Groschup Ute Ziegler |
author_sort |
Nicole Cichon |
title |
Serological and Molecular Investigation of Batai Virus Infections in Ruminants from the State of Saxony-Anhalt, Germany, 2018 |
title_short |
Serological and Molecular Investigation of Batai Virus Infections in Ruminants from the State of Saxony-Anhalt, Germany, 2018 |
title_full |
Serological and Molecular Investigation of Batai Virus Infections in Ruminants from the State of Saxony-Anhalt, Germany, 2018 |
title_fullStr |
Serological and Molecular Investigation of Batai Virus Infections in Ruminants from the State of Saxony-Anhalt, Germany, 2018 |
title_full_unstemmed |
Serological and Molecular Investigation of Batai Virus Infections in Ruminants from the State of Saxony-Anhalt, Germany, 2018 |
title_sort |
serological and molecular investigation of batai virus infections in ruminants from the state of saxony-anhalt, germany, 2018 |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/v13030370 https://doaj.org/article/4786d8f4a1344a0db89627d78856eed7 |
genre |
harbor seal |
genre_facet |
harbor seal |
op_source |
Viruses, Vol 13, Iss 3, p 370 (2021) |
op_relation |
https://www.mdpi.com/1999-4915/13/3/370 https://doaj.org/toc/1999-4915 doi:10.3390/v13030370 1999-4915 https://doaj.org/article/4786d8f4a1344a0db89627d78856eed7 |
op_doi |
https://doi.org/10.3390/v13030370 |
container_title |
Viruses |
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13 |
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3 |
container_start_page |
370 |
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1788061863281426432 |