Selective Stimulation of Duplicated Atlantic Salmon MHC Pathway Genes by Interferon-Gamma
Induction of cellular immune responses rely on Major histocompatibility complex (MHC) molecules presenting pathogenic peptides to T cells. Peptide processing, transport, loading and editing is a constitutive process in most cell types, but is accelerated upon infection. Recently, an unexpected compl...
Published in: | Frontiers in Immunology |
---|---|
Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Frontiers Media S.A.
2020
|
Subjects: | |
Online Access: | https://doi.org/10.3389/fimmu.2020.571650 https://doaj.org/article/470fc3db5cc044c5a3526da3bff4b8f0 |
id |
ftdoajarticles:oai:doaj.org/article:470fc3db5cc044c5a3526da3bff4b8f0 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:470fc3db5cc044c5a3526da3bff4b8f0 2023-05-15T15:30:32+02:00 Selective Stimulation of Duplicated Atlantic Salmon MHC Pathway Genes by Interferon-Gamma Unni Grimholt Johanna H. Fosse Arvind Y. M. Sundaram 2020-10-01T00:00:00Z https://doi.org/10.3389/fimmu.2020.571650 https://doaj.org/article/470fc3db5cc044c5a3526da3bff4b8f0 EN eng Frontiers Media S.A. https://www.frontiersin.org/article/10.3389/fimmu.2020.571650/full https://doaj.org/toc/1664-3224 1664-3224 doi:10.3389/fimmu.2020.571650 https://doaj.org/article/470fc3db5cc044c5a3526da3bff4b8f0 Frontiers in Immunology, Vol 11 (2020) MHC pathways whole genome duplications interferon gamma teleost antigen presentation homeologs Immunologic diseases. Allergy RC581-607 article 2020 ftdoajarticles https://doi.org/10.3389/fimmu.2020.571650 2022-12-31T00:12:24Z Induction of cellular immune responses rely on Major histocompatibility complex (MHC) molecules presenting pathogenic peptides to T cells. Peptide processing, transport, loading and editing is a constitutive process in most cell types, but is accelerated upon infection. Recently, an unexpected complexity in the number of functional genes involved in MHC class I peptide cleavage, peptide transport, peptide loading and editing was found in teleosts, originating from the second and third whole genome duplication events. Salmonids have expanded upon this with functional duplicates also from a fourth unique salmonid whole genome duplication. However, little is known about how individual gene duplicates respond in the context of stimulation. Here we set out to investigate how interferon gamma (IFNg) regulates the transcription of immune genes in Atlantic salmon with particular focus on gene duplicates and MHC pathways. We identified a range of response patterns in Atlantic salmon gene duplicates, with upregulation of all duplicates for some genes, like interferon regulatory factor 1 (IRF1) and interferon induced protein 44-like (IFI44.L), but only induction of one or a few duplicates of other genes, such as TAPBP and ERAP2. A master regulator turned out to be the IRF1 and not the enhanceosome as seen in mammals. If IRF1 also collaborates with CIITA and possibly NLRC5 in regulating IFNg induction of MHCI and MHCII expression in Atlantic salmon, as in zebrafish, remains to be established. Altogether, our results show the importance of deciphering between gene duplicates, as they often respond very differently to stimulation and may have different biological functions. Article in Journal/Newspaper Atlantic salmon Directory of Open Access Journals: DOAJ Articles Frontiers in Immunology 11 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
MHC pathways whole genome duplications interferon gamma teleost antigen presentation homeologs Immunologic diseases. Allergy RC581-607 |
spellingShingle |
MHC pathways whole genome duplications interferon gamma teleost antigen presentation homeologs Immunologic diseases. Allergy RC581-607 Unni Grimholt Johanna H. Fosse Arvind Y. M. Sundaram Selective Stimulation of Duplicated Atlantic Salmon MHC Pathway Genes by Interferon-Gamma |
topic_facet |
MHC pathways whole genome duplications interferon gamma teleost antigen presentation homeologs Immunologic diseases. Allergy RC581-607 |
description |
Induction of cellular immune responses rely on Major histocompatibility complex (MHC) molecules presenting pathogenic peptides to T cells. Peptide processing, transport, loading and editing is a constitutive process in most cell types, but is accelerated upon infection. Recently, an unexpected complexity in the number of functional genes involved in MHC class I peptide cleavage, peptide transport, peptide loading and editing was found in teleosts, originating from the second and third whole genome duplication events. Salmonids have expanded upon this with functional duplicates also from a fourth unique salmonid whole genome duplication. However, little is known about how individual gene duplicates respond in the context of stimulation. Here we set out to investigate how interferon gamma (IFNg) regulates the transcription of immune genes in Atlantic salmon with particular focus on gene duplicates and MHC pathways. We identified a range of response patterns in Atlantic salmon gene duplicates, with upregulation of all duplicates for some genes, like interferon regulatory factor 1 (IRF1) and interferon induced protein 44-like (IFI44.L), but only induction of one or a few duplicates of other genes, such as TAPBP and ERAP2. A master regulator turned out to be the IRF1 and not the enhanceosome as seen in mammals. If IRF1 also collaborates with CIITA and possibly NLRC5 in regulating IFNg induction of MHCI and MHCII expression in Atlantic salmon, as in zebrafish, remains to be established. Altogether, our results show the importance of deciphering between gene duplicates, as they often respond very differently to stimulation and may have different biological functions. |
format |
Article in Journal/Newspaper |
author |
Unni Grimholt Johanna H. Fosse Arvind Y. M. Sundaram |
author_facet |
Unni Grimholt Johanna H. Fosse Arvind Y. M. Sundaram |
author_sort |
Unni Grimholt |
title |
Selective Stimulation of Duplicated Atlantic Salmon MHC Pathway Genes by Interferon-Gamma |
title_short |
Selective Stimulation of Duplicated Atlantic Salmon MHC Pathway Genes by Interferon-Gamma |
title_full |
Selective Stimulation of Duplicated Atlantic Salmon MHC Pathway Genes by Interferon-Gamma |
title_fullStr |
Selective Stimulation of Duplicated Atlantic Salmon MHC Pathway Genes by Interferon-Gamma |
title_full_unstemmed |
Selective Stimulation of Duplicated Atlantic Salmon MHC Pathway Genes by Interferon-Gamma |
title_sort |
selective stimulation of duplicated atlantic salmon mhc pathway genes by interferon-gamma |
publisher |
Frontiers Media S.A. |
publishDate |
2020 |
url |
https://doi.org/10.3389/fimmu.2020.571650 https://doaj.org/article/470fc3db5cc044c5a3526da3bff4b8f0 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Frontiers in Immunology, Vol 11 (2020) |
op_relation |
https://www.frontiersin.org/article/10.3389/fimmu.2020.571650/full https://doaj.org/toc/1664-3224 1664-3224 doi:10.3389/fimmu.2020.571650 https://doaj.org/article/470fc3db5cc044c5a3526da3bff4b8f0 |
op_doi |
https://doi.org/10.3389/fimmu.2020.571650 |
container_title |
Frontiers in Immunology |
container_volume |
11 |
_version_ |
1766360980903690240 |