Detection of Bartonella spp. in fleas by MALDI-TOF MS.
Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) has recently emerged in the field of entomology as a promising method for the identification of arthropods and the detection of associated pathogens.An experimental model of Ctenocephalides felis (cat fleas)...
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ftdoajarticles:oai:doaj.org/article:470308de88714ca3963800af4f264cc2 2023-05-15T15:16:36+02:00 Detection of Bartonella spp. in fleas by MALDI-TOF MS. Basma El Hamzaoui Maureen Laroche Lionel Almeras Jean-Michel Bérenger Didier Raoult Philippe Parola 2018-02-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0006189 https://doaj.org/article/470308de88714ca3963800af4f264cc2 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5833284?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006189 https://doaj.org/article/470308de88714ca3963800af4f264cc2 PLoS Neglected Tropical Diseases, Vol 12, Iss 2, p e0006189 (2018) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2018 ftdoajarticles https://doi.org/10.1371/journal.pntd.0006189 2022-12-31T12:24:11Z Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) has recently emerged in the field of entomology as a promising method for the identification of arthropods and the detection of associated pathogens.An experimental model of Ctenocephalides felis (cat fleas) infected with Bartonella quintana and Bartonella henselae was developed to evaluate the efficacy of MALDI-TOF MS in distinguishing infected from uninfected fleas, and its ability to distinguish fleas infected with Bartonella quintana from fleas infected with Bartonella henselae. For B. quintana, two groups of fleas received three successive blood meals, infected or not. A total of 140 fleas (100 exposed fleas and 40 control fleas) were engorged on human blood, infected or uninfected with B. quintana. Regarding the second pathogen, two groups of fleas (200 exposed fleas and 40 control fleas) were fed in the same manner with human blood, infected or not with Bartonella henselae. Fleas were dissected longitudinally; one-half was used for assessment of B. quintana and B. henselae infectious status by real-time PCR, and the second half was subjected to MALDI-TOF MS analysis. Comparison of MS spectra from infected fleas and uninfected fleas revealed distinct MS profiles. Blind queries against our MALDI-TOF MS arthropod database, upgraded with reference spectra from B. quintana and B. henselae infected fleas but also non-infected fleas, provided the correct classification for 100% of the different categories of specimens tested on the first model of flea infection with Bartonella quintana. As for Bartonella henselae, 81% of exposed qPCR-positive fleas, 96% of exposed qPCR-negative fleas and 100% of control fleas were correctly identified on the second model of flea infection. MALDI-TOF MS successfully differentiated Bartonella spp.-infected and uninfected fleas and was also able to correctly differentiate fleas infected with Bartonella quintana and fleas infected with Bartonella henselae. MALDI-TOF MS correctly identified ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 12 2 e0006189 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Basma El Hamzaoui Maureen Laroche Lionel Almeras Jean-Michel Bérenger Didier Raoult Philippe Parola Detection of Bartonella spp. in fleas by MALDI-TOF MS. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) has recently emerged in the field of entomology as a promising method for the identification of arthropods and the detection of associated pathogens.An experimental model of Ctenocephalides felis (cat fleas) infected with Bartonella quintana and Bartonella henselae was developed to evaluate the efficacy of MALDI-TOF MS in distinguishing infected from uninfected fleas, and its ability to distinguish fleas infected with Bartonella quintana from fleas infected with Bartonella henselae. For B. quintana, two groups of fleas received three successive blood meals, infected or not. A total of 140 fleas (100 exposed fleas and 40 control fleas) were engorged on human blood, infected or uninfected with B. quintana. Regarding the second pathogen, two groups of fleas (200 exposed fleas and 40 control fleas) were fed in the same manner with human blood, infected or not with Bartonella henselae. Fleas were dissected longitudinally; one-half was used for assessment of B. quintana and B. henselae infectious status by real-time PCR, and the second half was subjected to MALDI-TOF MS analysis. Comparison of MS spectra from infected fleas and uninfected fleas revealed distinct MS profiles. Blind queries against our MALDI-TOF MS arthropod database, upgraded with reference spectra from B. quintana and B. henselae infected fleas but also non-infected fleas, provided the correct classification for 100% of the different categories of specimens tested on the first model of flea infection with Bartonella quintana. As for Bartonella henselae, 81% of exposed qPCR-positive fleas, 96% of exposed qPCR-negative fleas and 100% of control fleas were correctly identified on the second model of flea infection. MALDI-TOF MS successfully differentiated Bartonella spp.-infected and uninfected fleas and was also able to correctly differentiate fleas infected with Bartonella quintana and fleas infected with Bartonella henselae. MALDI-TOF MS correctly identified ... |
format |
Article in Journal/Newspaper |
author |
Basma El Hamzaoui Maureen Laroche Lionel Almeras Jean-Michel Bérenger Didier Raoult Philippe Parola |
author_facet |
Basma El Hamzaoui Maureen Laroche Lionel Almeras Jean-Michel Bérenger Didier Raoult Philippe Parola |
author_sort |
Basma El Hamzaoui |
title |
Detection of Bartonella spp. in fleas by MALDI-TOF MS. |
title_short |
Detection of Bartonella spp. in fleas by MALDI-TOF MS. |
title_full |
Detection of Bartonella spp. in fleas by MALDI-TOF MS. |
title_fullStr |
Detection of Bartonella spp. in fleas by MALDI-TOF MS. |
title_full_unstemmed |
Detection of Bartonella spp. in fleas by MALDI-TOF MS. |
title_sort |
detection of bartonella spp. in fleas by maldi-tof ms. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2018 |
url |
https://doi.org/10.1371/journal.pntd.0006189 https://doaj.org/article/470308de88714ca3963800af4f264cc2 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 12, Iss 2, p e0006189 (2018) |
op_relation |
http://europepmc.org/articles/PMC5833284?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006189 https://doaj.org/article/470308de88714ca3963800af4f264cc2 |
op_doi |
https://doi.org/10.1371/journal.pntd.0006189 |
container_title |
PLOS Neglected Tropical Diseases |
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12 |
container_issue |
2 |
container_start_page |
e0006189 |
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1766346893637451776 |