Screening Study for Anticonvulsive Activity of Lipophilic Fractions from Empetrum nigrum L.
Introduction: The plants of genus Empetrum, which are used in the traditional medicine to cure seizures and neurodegenerative diseases, can be considered as potent antiepileptic drugs. This paper focuses on a comparative analysis of an anticonvulsive activity of lipophilic fractions from Empetrum ni...
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ftdoajarticles:oai:doaj.org/article:469865d0db344471bb3355ff8faa4a91 2023-05-15T16:06:03+02:00 Screening Study for Anticonvulsive Activity of Lipophilic Fractions from Empetrum nigrum L. Ekaterina A. Bezverkhniaia Tatyana N. Povet’eva Tatyana V. Kadyrova Nikolai I. Suslov Yuliya V. Nesterova Ol’ga G. Afanas’eva Pavel V. Kul’pin Yuliya G. Yusova Elena V. Ermilova Alexandr G. Miroshnichenko Konstantin S. Brazovskii Mikhail V. Belousov 2020-09-01T00:00:00Z https://doi.org/10.3897/rrpharmacology.6.55015 https://doaj.org/article/469865d0db344471bb3355ff8faa4a91 EN eng Pensoft Publishers https://rrpharmacology.pensoft.net/article/55015/download/pdf/ https://rrpharmacology.pensoft.net/article/55015/download/xml/ https://rrpharmacology.pensoft.net/article/55015/ https://doaj.org/toc/2658-381X doi:10.3897/rrpharmacology.6.55015 2658-381X https://doaj.org/article/469865d0db344471bb3355ff8faa4a91 Research Results in Pharmacology, Vol 6, Iss 3, Pp 67-74 (2020) Therapeutics. Pharmacology RM1-950 article 2020 ftdoajarticles https://doi.org/10.3897/rrpharmacology.6.55015 2022-12-31T14:20:30Z Introduction: The plants of genus Empetrum, which are used in the traditional medicine to cure seizures and neurodegenerative diseases, can be considered as potent antiepileptic drugs. This paper focuses on a comparative analysis of an anticonvulsive activity of lipophilic fractions from Empetrum nigrum L. Materials and methods: The experiments were conducted using mature outbred CD-1 male mice. The lipophilic fractions from aerial parts of Empetrum nigrum L. were administered through a catheter into the stomach at a dose of 150 mg/kg for 5 days. The anticonvulsive effects were studied using the acute seizure tests: strychnine-, pentylenetetrazole – and maximal electroshock (MES) induced tests. Carbamazepine was used as a positive control drug at a dose of 100 mg/kg. Results and discussion: The acetone-soluble fraction (ASF) of the chloroform extract from Empetrum nigrum L. showed a pronounced anticonvulsive effect on seizures induced by strychnine (1.5 mg/kg) and pentylenetetrazole (150 mg/kg). In comparison to the control group, the time from seizures to death increased by 1.5 for the strychnine-induced seizures, and 1.9 times in case of pentylenetetrazole model. The survival rate of the animals was 22.2% and 20%, correspondingly. The survival rate in the MES test was 77.8%. Overall, ASF demonstrates a remarkable anticonvulsive activity in all the tests, especially in the MES test. Conclusion: Our study for the first time shows a potent antiepileptic effect of ASF from Empetrum nigrum L., containing triterpene compounds and chalcones. The future studies will be focused on investigating the exact mechanisms of anticonvulsive and neuroprotective effects of ASF. Graphical abstract Article in Journal/Newspaper Empetrum nigrum Directory of Open Access Journals: DOAJ Articles Research Results in Pharmacology 6 3 67 74 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Therapeutics. Pharmacology RM1-950 |
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Therapeutics. Pharmacology RM1-950 Ekaterina A. Bezverkhniaia Tatyana N. Povet’eva Tatyana V. Kadyrova Nikolai I. Suslov Yuliya V. Nesterova Ol’ga G. Afanas’eva Pavel V. Kul’pin Yuliya G. Yusova Elena V. Ermilova Alexandr G. Miroshnichenko Konstantin S. Brazovskii Mikhail V. Belousov Screening Study for Anticonvulsive Activity of Lipophilic Fractions from Empetrum nigrum L. |
topic_facet |
Therapeutics. Pharmacology RM1-950 |
description |
Introduction: The plants of genus Empetrum, which are used in the traditional medicine to cure seizures and neurodegenerative diseases, can be considered as potent antiepileptic drugs. This paper focuses on a comparative analysis of an anticonvulsive activity of lipophilic fractions from Empetrum nigrum L. Materials and methods: The experiments were conducted using mature outbred CD-1 male mice. The lipophilic fractions from aerial parts of Empetrum nigrum L. were administered through a catheter into the stomach at a dose of 150 mg/kg for 5 days. The anticonvulsive effects were studied using the acute seizure tests: strychnine-, pentylenetetrazole – and maximal electroshock (MES) induced tests. Carbamazepine was used as a positive control drug at a dose of 100 mg/kg. Results and discussion: The acetone-soluble fraction (ASF) of the chloroform extract from Empetrum nigrum L. showed a pronounced anticonvulsive effect on seizures induced by strychnine (1.5 mg/kg) and pentylenetetrazole (150 mg/kg). In comparison to the control group, the time from seizures to death increased by 1.5 for the strychnine-induced seizures, and 1.9 times in case of pentylenetetrazole model. The survival rate of the animals was 22.2% and 20%, correspondingly. The survival rate in the MES test was 77.8%. Overall, ASF demonstrates a remarkable anticonvulsive activity in all the tests, especially in the MES test. Conclusion: Our study for the first time shows a potent antiepileptic effect of ASF from Empetrum nigrum L., containing triterpene compounds and chalcones. The future studies will be focused on investigating the exact mechanisms of anticonvulsive and neuroprotective effects of ASF. Graphical abstract |
format |
Article in Journal/Newspaper |
author |
Ekaterina A. Bezverkhniaia Tatyana N. Povet’eva Tatyana V. Kadyrova Nikolai I. Suslov Yuliya V. Nesterova Ol’ga G. Afanas’eva Pavel V. Kul’pin Yuliya G. Yusova Elena V. Ermilova Alexandr G. Miroshnichenko Konstantin S. Brazovskii Mikhail V. Belousov |
author_facet |
Ekaterina A. Bezverkhniaia Tatyana N. Povet’eva Tatyana V. Kadyrova Nikolai I. Suslov Yuliya V. Nesterova Ol’ga G. Afanas’eva Pavel V. Kul’pin Yuliya G. Yusova Elena V. Ermilova Alexandr G. Miroshnichenko Konstantin S. Brazovskii Mikhail V. Belousov |
author_sort |
Ekaterina A. Bezverkhniaia |
title |
Screening Study for Anticonvulsive Activity of Lipophilic Fractions from Empetrum nigrum L. |
title_short |
Screening Study for Anticonvulsive Activity of Lipophilic Fractions from Empetrum nigrum L. |
title_full |
Screening Study for Anticonvulsive Activity of Lipophilic Fractions from Empetrum nigrum L. |
title_fullStr |
Screening Study for Anticonvulsive Activity of Lipophilic Fractions from Empetrum nigrum L. |
title_full_unstemmed |
Screening Study for Anticonvulsive Activity of Lipophilic Fractions from Empetrum nigrum L. |
title_sort |
screening study for anticonvulsive activity of lipophilic fractions from empetrum nigrum l. |
publisher |
Pensoft Publishers |
publishDate |
2020 |
url |
https://doi.org/10.3897/rrpharmacology.6.55015 https://doaj.org/article/469865d0db344471bb3355ff8faa4a91 |
genre |
Empetrum nigrum |
genre_facet |
Empetrum nigrum |
op_source |
Research Results in Pharmacology, Vol 6, Iss 3, Pp 67-74 (2020) |
op_relation |
https://rrpharmacology.pensoft.net/article/55015/download/pdf/ https://rrpharmacology.pensoft.net/article/55015/download/xml/ https://rrpharmacology.pensoft.net/article/55015/ https://doaj.org/toc/2658-381X doi:10.3897/rrpharmacology.6.55015 2658-381X https://doaj.org/article/469865d0db344471bb3355ff8faa4a91 |
op_doi |
https://doi.org/10.3897/rrpharmacology.6.55015 |
container_title |
Research Results in Pharmacology |
container_volume |
6 |
container_issue |
3 |
container_start_page |
67 |
op_container_end_page |
74 |
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1766401960353726464 |