Using a top predator as a sentinel for environmental contamination with pathogenic bacteria: the Iberian wolf and leptospires
The Iberian wolf (Canis lupus) is the top predator in the Iberian environments in which it lives, feeding on a wide range of species, thus encountering a wide range of disease agents. Therefore, the wolf can serve as sentinel of environmental contamination with pathogens. We investigated the exposur...
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Online Access: | https://doi.org/10.1590/0074-0276140258 https://doaj.org/article/4698517f825d479cb6ee5528ae2a5fa8 |
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ftdoajarticles:oai:doaj.org/article:4698517f825d479cb6ee5528ae2a5fa8 2023-08-27T04:08:54+02:00 Using a top predator as a sentinel for environmental contamination with pathogenic bacteria: the Iberian wolf and leptospires Javier Millán Emilio J García Álvaro Oleaga José Vicente López-Bao Luis Llaneza Vicente Palacios Mónica G Candela Aitor Cevidanes Alejandro Rodríguez Luis León-Vizcaíno 2014-12-01T00:00:00Z https://doi.org/10.1590/0074-0276140258 https://doaj.org/article/4698517f825d479cb6ee5528ae2a5fa8 EN eng Fundação Oswaldo Cruz (FIOCRUZ) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000801041&lng=en&tlng=en https://doaj.org/toc/1678-8060 1678-8060 doi:10.1590/0074-0276140258 https://doaj.org/article/4698517f825d479cb6ee5528ae2a5fa8 Memorias do Instituto Oswaldo Cruz, Vol 109, Iss 8, Pp 1041-1044 (2014) Carnivora Leptospira borgpetersenii spirochete Microbiology QR1-502 Infectious and parasitic diseases RC109-216 article 2014 ftdoajarticles https://doi.org/10.1590/0074-0276140258 2023-08-06T00:39:47Z The Iberian wolf (Canis lupus) is the top predator in the Iberian environments in which it lives, feeding on a wide range of species, thus encountering a wide range of disease agents. Therefore, the wolf can serve as sentinel of environmental contamination with pathogens. We investigated the exposure of free-living wolves to 14 serovars of Leptospira interrogans sensu lato. Kidney samples from 49 wolves collected from 2010-2013 in northwestern Spain were analysed by culture, direct immunofluorescence and polymerase chain reaction. Tissue fluids were analysed for antibodies by a microscopic agglutination test. Ten wolves (observed prevalence: 20%, 95% confidence interval = 11-33%) showed evidence of contact with leptospires, eight through direct detection and nine through serology (7 wolves were positive according to both techniques). Titres below the cut-off level were also detected in seven cases. Serovars confirmed were Canicola (n = 4), Icterohaemorrhagiae (n = 3) and Sejroë, Ballum and Grippotyphosa (n = 1 each), indicating that wolves were infected with serovars for which dogs, rodents and ungulates, are the natural hosts and supporting the utility of the wolf and other large predators as environmental sentinels for pathogens. Article in Journal/Newspaper Canis lupus Directory of Open Access Journals: DOAJ Articles Memórias do Instituto Oswaldo Cruz 109 8 1041 1044 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Carnivora Leptospira borgpetersenii spirochete Microbiology QR1-502 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Carnivora Leptospira borgpetersenii spirochete Microbiology QR1-502 Infectious and parasitic diseases RC109-216 Javier Millán Emilio J García Álvaro Oleaga José Vicente López-Bao Luis Llaneza Vicente Palacios Mónica G Candela Aitor Cevidanes Alejandro Rodríguez Luis León-Vizcaíno Using a top predator as a sentinel for environmental contamination with pathogenic bacteria: the Iberian wolf and leptospires |
topic_facet |
Carnivora Leptospira borgpetersenii spirochete Microbiology QR1-502 Infectious and parasitic diseases RC109-216 |
description |
The Iberian wolf (Canis lupus) is the top predator in the Iberian environments in which it lives, feeding on a wide range of species, thus encountering a wide range of disease agents. Therefore, the wolf can serve as sentinel of environmental contamination with pathogens. We investigated the exposure of free-living wolves to 14 serovars of Leptospira interrogans sensu lato. Kidney samples from 49 wolves collected from 2010-2013 in northwestern Spain were analysed by culture, direct immunofluorescence and polymerase chain reaction. Tissue fluids were analysed for antibodies by a microscopic agglutination test. Ten wolves (observed prevalence: 20%, 95% confidence interval = 11-33%) showed evidence of contact with leptospires, eight through direct detection and nine through serology (7 wolves were positive according to both techniques). Titres below the cut-off level were also detected in seven cases. Serovars confirmed were Canicola (n = 4), Icterohaemorrhagiae (n = 3) and Sejroë, Ballum and Grippotyphosa (n = 1 each), indicating that wolves were infected with serovars for which dogs, rodents and ungulates, are the natural hosts and supporting the utility of the wolf and other large predators as environmental sentinels for pathogens. |
format |
Article in Journal/Newspaper |
author |
Javier Millán Emilio J García Álvaro Oleaga José Vicente López-Bao Luis Llaneza Vicente Palacios Mónica G Candela Aitor Cevidanes Alejandro Rodríguez Luis León-Vizcaíno |
author_facet |
Javier Millán Emilio J García Álvaro Oleaga José Vicente López-Bao Luis Llaneza Vicente Palacios Mónica G Candela Aitor Cevidanes Alejandro Rodríguez Luis León-Vizcaíno |
author_sort |
Javier Millán |
title |
Using a top predator as a sentinel for environmental contamination with pathogenic bacteria: the Iberian wolf and leptospires |
title_short |
Using a top predator as a sentinel for environmental contamination with pathogenic bacteria: the Iberian wolf and leptospires |
title_full |
Using a top predator as a sentinel for environmental contamination with pathogenic bacteria: the Iberian wolf and leptospires |
title_fullStr |
Using a top predator as a sentinel for environmental contamination with pathogenic bacteria: the Iberian wolf and leptospires |
title_full_unstemmed |
Using a top predator as a sentinel for environmental contamination with pathogenic bacteria: the Iberian wolf and leptospires |
title_sort |
using a top predator as a sentinel for environmental contamination with pathogenic bacteria: the iberian wolf and leptospires |
publisher |
Fundação Oswaldo Cruz (FIOCRUZ) |
publishDate |
2014 |
url |
https://doi.org/10.1590/0074-0276140258 https://doaj.org/article/4698517f825d479cb6ee5528ae2a5fa8 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Memorias do Instituto Oswaldo Cruz, Vol 109, Iss 8, Pp 1041-1044 (2014) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000801041&lng=en&tlng=en https://doaj.org/toc/1678-8060 1678-8060 doi:10.1590/0074-0276140258 https://doaj.org/article/4698517f825d479cb6ee5528ae2a5fa8 |
op_doi |
https://doi.org/10.1590/0074-0276140258 |
container_title |
Memórias do Instituto Oswaldo Cruz |
container_volume |
109 |
container_issue |
8 |
container_start_page |
1041 |
op_container_end_page |
1044 |
_version_ |
1775349827000860672 |