Role of DEAD-box RNA helicases in low-temperature adapted growth of Antarctic Pseudomonas syringae Lz4W
ABSTRACT Pseudomonas syringae possesses genes for all five major DEAD-box RNA helicases, rhlE, srmB, csdA, dbpA, and rhlB, that are found in γ-proteobacteria. Bioinformatic analysis of the RNA helicase genes provided detailed insights into their genomic organization, promoter characteristics, regula...
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American Society for Microbiology
2024
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ftdoajarticles:oai:doaj.org/article:4690f8778b7e480fb9f95cc3bfbd2d48 2024-02-11T09:58:48+01:00 Role of DEAD-box RNA helicases in low-temperature adapted growth of Antarctic Pseudomonas syringae Lz4W Ashaq Hussain Malay Kumar Ray 2024-01-01T00:00:00Z https://doi.org/10.1128/spectrum.04335-22 https://doaj.org/article/4690f8778b7e480fb9f95cc3bfbd2d48 EN eng American Society for Microbiology https://journals.asm.org/doi/10.1128/spectrum.04335-22 https://doaj.org/toc/2165-0497 doi:10.1128/spectrum.04335-22 2165-0497 https://doaj.org/article/4690f8778b7e480fb9f95cc3bfbd2d48 Microbiology Spectrum, Vol 12, Iss 1 (2024) cold adaptation RNA helicases gene disruption homologous recombination growth analysis functional complementation Microbiology QR1-502 article 2024 ftdoajarticles https://doi.org/10.1128/spectrum.04335-22 2024-01-14T01:38:03Z ABSTRACT Pseudomonas syringae possesses genes for all five major DEAD-box RNA helicases, rhlE, srmB, csdA, dbpA, and rhlB, that are found in γ-proteobacteria. Bioinformatic analysis of the RNA helicase genes provided detailed insights into their genomic organization, promoter characteristics, regulatory 3′ regions, and sequence similarity. Disruption of rhlB and rhlE genes did not have any effect on growth of the P. syringae mutants at optimum (22°C) or at low (4°C) temperatures. On the other hand, disruption of srmB and dbpA genes caused a slow-growing phenotype in the mutants at low temperature (4°C). In comparison to the above-mentioned helicases, deletion of csdA gene caused the mutant (ΔcsdA) to be totally incapable of growing at 4°C while marginally affecting the growth (resulting in slow growth) at the optimum temperature (22°C). Functional complementation studies revealed that RNA helicases are functionally non-redundant, as the roles performed by different helicases are individual and specific. IMPORTANCE RNA metabolism is important as RNA acts as a link between genomic information and functional biomolecules, thereby playing a critical role in cellular response to environment. We investigated the role of DEAD-box RNA helicases in low-temperature adapted growth of P. syringae, as this group of enzymes play an essential role in modulation of RNA secondary structures. This is the first report on the assessment of all major DEAD-box RNA helicases in any Antarctic bacterium. Of the five RNA helicases, three (srmB, csdA, and dbpA) are important for the growth of the Antarctic P. syringae at low temperature. However, the requisite role of dbpA and the indispensable requirement of csdA for low-temperature adapted growth are a novel finding of this study. Growth analysis of combinatorial deletion strains was performed to understand the functional interaction among helicase genes. Similarly, genetic complementation of RNA helicase mutants was conducted for identification of gene redundancy in P. syringae. Article in Journal/Newspaper Antarc* Antarctic Directory of Open Access Journals: DOAJ Articles Antarctic The Antarctic Microbiology Spectrum 12 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
cold adaptation RNA helicases gene disruption homologous recombination growth analysis functional complementation Microbiology QR1-502 |
spellingShingle |
cold adaptation RNA helicases gene disruption homologous recombination growth analysis functional complementation Microbiology QR1-502 Ashaq Hussain Malay Kumar Ray Role of DEAD-box RNA helicases in low-temperature adapted growth of Antarctic Pseudomonas syringae Lz4W |
topic_facet |
cold adaptation RNA helicases gene disruption homologous recombination growth analysis functional complementation Microbiology QR1-502 |
description |
ABSTRACT Pseudomonas syringae possesses genes for all five major DEAD-box RNA helicases, rhlE, srmB, csdA, dbpA, and rhlB, that are found in γ-proteobacteria. Bioinformatic analysis of the RNA helicase genes provided detailed insights into their genomic organization, promoter characteristics, regulatory 3′ regions, and sequence similarity. Disruption of rhlB and rhlE genes did not have any effect on growth of the P. syringae mutants at optimum (22°C) or at low (4°C) temperatures. On the other hand, disruption of srmB and dbpA genes caused a slow-growing phenotype in the mutants at low temperature (4°C). In comparison to the above-mentioned helicases, deletion of csdA gene caused the mutant (ΔcsdA) to be totally incapable of growing at 4°C while marginally affecting the growth (resulting in slow growth) at the optimum temperature (22°C). Functional complementation studies revealed that RNA helicases are functionally non-redundant, as the roles performed by different helicases are individual and specific. IMPORTANCE RNA metabolism is important as RNA acts as a link between genomic information and functional biomolecules, thereby playing a critical role in cellular response to environment. We investigated the role of DEAD-box RNA helicases in low-temperature adapted growth of P. syringae, as this group of enzymes play an essential role in modulation of RNA secondary structures. This is the first report on the assessment of all major DEAD-box RNA helicases in any Antarctic bacterium. Of the five RNA helicases, three (srmB, csdA, and dbpA) are important for the growth of the Antarctic P. syringae at low temperature. However, the requisite role of dbpA and the indispensable requirement of csdA for low-temperature adapted growth are a novel finding of this study. Growth analysis of combinatorial deletion strains was performed to understand the functional interaction among helicase genes. Similarly, genetic complementation of RNA helicase mutants was conducted for identification of gene redundancy in P. syringae. |
format |
Article in Journal/Newspaper |
author |
Ashaq Hussain Malay Kumar Ray |
author_facet |
Ashaq Hussain Malay Kumar Ray |
author_sort |
Ashaq Hussain |
title |
Role of DEAD-box RNA helicases in low-temperature adapted growth of Antarctic Pseudomonas syringae Lz4W |
title_short |
Role of DEAD-box RNA helicases in low-temperature adapted growth of Antarctic Pseudomonas syringae Lz4W |
title_full |
Role of DEAD-box RNA helicases in low-temperature adapted growth of Antarctic Pseudomonas syringae Lz4W |
title_fullStr |
Role of DEAD-box RNA helicases in low-temperature adapted growth of Antarctic Pseudomonas syringae Lz4W |
title_full_unstemmed |
Role of DEAD-box RNA helicases in low-temperature adapted growth of Antarctic Pseudomonas syringae Lz4W |
title_sort |
role of dead-box rna helicases in low-temperature adapted growth of antarctic pseudomonas syringae lz4w |
publisher |
American Society for Microbiology |
publishDate |
2024 |
url |
https://doi.org/10.1128/spectrum.04335-22 https://doaj.org/article/4690f8778b7e480fb9f95cc3bfbd2d48 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Microbiology Spectrum, Vol 12, Iss 1 (2024) |
op_relation |
https://journals.asm.org/doi/10.1128/spectrum.04335-22 https://doaj.org/toc/2165-0497 doi:10.1128/spectrum.04335-22 2165-0497 https://doaj.org/article/4690f8778b7e480fb9f95cc3bfbd2d48 |
op_doi |
https://doi.org/10.1128/spectrum.04335-22 |
container_title |
Microbiology Spectrum |
container_volume |
12 |
container_issue |
1 |
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1790594563572760576 |