Microarray analysis of microbiota of gingival lesions in noma patients.
Noma (cancrum oris) is a gangrenous disease of unknown etiology affecting the maxillo-facial region of young children in extremely limited resource countries. In an attempt to better understand the microbiological events occurring during this disease, we used phylogenetic and low-density microarrays...
Published in: | PLoS Neglected Tropical Diseases |
---|---|
Main Authors: | , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2013
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pntd.0002453 https://doaj.org/article/468b4e336cb242078141c11f4e0b3223 |
id |
ftdoajarticles:oai:doaj.org/article:468b4e336cb242078141c11f4e0b3223 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:468b4e336cb242078141c11f4e0b3223 2023-05-15T15:06:46+02:00 Microarray analysis of microbiota of gingival lesions in noma patients. Antoine Huyghe Patrice François Andrea Mombelli Manuela Tangomo Myriam Girard Denise Baratti-Mayer Ignacio Bolivar Didier Pittet Jacques Schrenzel Geneva Study Group on Noma 2013-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0002453 https://doaj.org/article/468b4e336cb242078141c11f4e0b3223 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3784469?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002453 https://doaj.org/article/468b4e336cb242078141c11f4e0b3223 PLoS Neglected Tropical Diseases, Vol 7, Iss 9, p e2453 (2013) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2013 ftdoajarticles https://doi.org/10.1371/journal.pntd.0002453 2022-12-30T21:57:33Z Noma (cancrum oris) is a gangrenous disease of unknown etiology affecting the maxillo-facial region of young children in extremely limited resource countries. In an attempt to better understand the microbiological events occurring during this disease, we used phylogenetic and low-density microarrays targeting the 16S rRNA gene to characterize the gingival flora of acute noma and acute necrotizing gingivitis (ANG) lesions, and compared them to healthy control subjects of the same geographical and social background. Our observations raise doubts about Fusobacterium necrophorum, a previously suspected causative agent of noma, as this species was not associated with noma lesions. Various oral pathogens were more abundant in noma lesions, notably Atopobium spp., Prevotella intermedia, Peptostreptococcus spp., Streptococcus pyogenes and Streptococcus anginosus. On the other hand, pathogens associated with periodontal diseases such as Aggregatibacter actinomycetemcomitans, Capnocytophaga spp., Porphyromonas spp. and Fusobacteriales were more abundant in healthy controls. Importantly, the overall loss of bacterial diversity observed in noma samples as well as its homology to that of ANG microbiota supports the hypothesis that ANG might be the immediate step preceding noma. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 7 9 e2453 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Antoine Huyghe Patrice François Andrea Mombelli Manuela Tangomo Myriam Girard Denise Baratti-Mayer Ignacio Bolivar Didier Pittet Jacques Schrenzel Geneva Study Group on Noma Microarray analysis of microbiota of gingival lesions in noma patients. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Noma (cancrum oris) is a gangrenous disease of unknown etiology affecting the maxillo-facial region of young children in extremely limited resource countries. In an attempt to better understand the microbiological events occurring during this disease, we used phylogenetic and low-density microarrays targeting the 16S rRNA gene to characterize the gingival flora of acute noma and acute necrotizing gingivitis (ANG) lesions, and compared them to healthy control subjects of the same geographical and social background. Our observations raise doubts about Fusobacterium necrophorum, a previously suspected causative agent of noma, as this species was not associated with noma lesions. Various oral pathogens were more abundant in noma lesions, notably Atopobium spp., Prevotella intermedia, Peptostreptococcus spp., Streptococcus pyogenes and Streptococcus anginosus. On the other hand, pathogens associated with periodontal diseases such as Aggregatibacter actinomycetemcomitans, Capnocytophaga spp., Porphyromonas spp. and Fusobacteriales were more abundant in healthy controls. Importantly, the overall loss of bacterial diversity observed in noma samples as well as its homology to that of ANG microbiota supports the hypothesis that ANG might be the immediate step preceding noma. |
format |
Article in Journal/Newspaper |
author |
Antoine Huyghe Patrice François Andrea Mombelli Manuela Tangomo Myriam Girard Denise Baratti-Mayer Ignacio Bolivar Didier Pittet Jacques Schrenzel Geneva Study Group on Noma |
author_facet |
Antoine Huyghe Patrice François Andrea Mombelli Manuela Tangomo Myriam Girard Denise Baratti-Mayer Ignacio Bolivar Didier Pittet Jacques Schrenzel Geneva Study Group on Noma |
author_sort |
Antoine Huyghe |
title |
Microarray analysis of microbiota of gingival lesions in noma patients. |
title_short |
Microarray analysis of microbiota of gingival lesions in noma patients. |
title_full |
Microarray analysis of microbiota of gingival lesions in noma patients. |
title_fullStr |
Microarray analysis of microbiota of gingival lesions in noma patients. |
title_full_unstemmed |
Microarray analysis of microbiota of gingival lesions in noma patients. |
title_sort |
microarray analysis of microbiota of gingival lesions in noma patients. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2013 |
url |
https://doi.org/10.1371/journal.pntd.0002453 https://doaj.org/article/468b4e336cb242078141c11f4e0b3223 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 7, Iss 9, p e2453 (2013) |
op_relation |
http://europepmc.org/articles/PMC3784469?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002453 https://doaj.org/article/468b4e336cb242078141c11f4e0b3223 |
op_doi |
https://doi.org/10.1371/journal.pntd.0002453 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
7 |
container_issue |
9 |
container_start_page |
e2453 |
_version_ |
1766338324622999552 |