Zn, Cu, Cd and Hg binding to metallothioneins in harbour porpoises Phocoena phocoena from the southern North Sea
Abstract Background Harbour porpoises Phocoena phocoena from the southern North Sea are known to display high levels of Zn and Hg in their tissues linked to their nutritional status (emaciation). The question arises regarding a potential role of metallothioneins (MTs) with regard to these high metal...
Published in: | BMC Ecology |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
BMC
2006
|
Subjects: | |
Online Access: | https://doi.org/10.1186/1472-6785-6-2 https://doaj.org/article/4677c191d20441adb6f961b471b7df5d |
id |
ftdoajarticles:oai:doaj.org/article:4677c191d20441adb6f961b471b7df5d |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:4677c191d20441adb6f961b471b7df5d 2023-05-15T17:59:09+02:00 Zn, Cu, Cd and Hg binding to metallothioneins in harbour porpoises Phocoena phocoena from the southern North Sea Jauniaux Thierry De Groof Arnaud Das Krishna Bouquegneau Jean-Marie 2006-02-01T00:00:00Z https://doi.org/10.1186/1472-6785-6-2 https://doaj.org/article/4677c191d20441adb6f961b471b7df5d EN eng BMC http://www.biomedcentral.com/1472-6785/6/2 https://doaj.org/toc/1472-6785 doi:10.1186/1472-6785-6-2 1472-6785 https://doaj.org/article/4677c191d20441adb6f961b471b7df5d BMC Ecology, Vol 6, Iss 1, p 2 (2006) Ecology QH540-549.5 article 2006 ftdoajarticles https://doi.org/10.1186/1472-6785-6-2 2022-12-31T16:19:08Z Abstract Background Harbour porpoises Phocoena phocoena from the southern North Sea are known to display high levels of Zn and Hg in their tissues linked to their nutritional status (emaciation). The question arises regarding a potential role of metallothioneins (MTs) with regard to these high metal levels. In the present study, metallothionein detection and associated Zn, Cd, Cu and Hg concentrations were investigated in the liver and kidney of 14 harbour porpoises collected along the Belgian coast. Results Metallothioneins seemed to play a key role in essential metal homeostasis, as they were shown to bind 50% of the total hepatic Zn and 36% of the total hepatic Cu concentrations. Renal MTs also participated in Cd detoxification, as they were shown to bind 56% of the total renal Cd. Hg was mainly found in the insoluble fraction of both liver and kidney. Concomitant increases in total Zn concentration and Zn bound to MTs were observed in the liver, whereas Zn concentration bound to high molecular weight proteins remained constant. Cu, Zn and Cd were accumulated preferentially in the MT fraction and their content in this fraction increased with the amount in the hepatocytosol. Conclusion MTs have a key role in Zn and Cu homeostasis in harbour porpoises. We demonstrated that increasing hepatic Zn concentration led to an increase in Zn linked to MTs, suggesting that these small proteins take over the Zn overload linked to the poor body condition of debilitated harbour porpoises. Article in Journal/Newspaper Phocoena phocoena Directory of Open Access Journals: DOAJ Articles BMC Ecology 6 1 2 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Ecology QH540-549.5 |
spellingShingle |
Ecology QH540-549.5 Jauniaux Thierry De Groof Arnaud Das Krishna Bouquegneau Jean-Marie Zn, Cu, Cd and Hg binding to metallothioneins in harbour porpoises Phocoena phocoena from the southern North Sea |
topic_facet |
Ecology QH540-549.5 |
description |
Abstract Background Harbour porpoises Phocoena phocoena from the southern North Sea are known to display high levels of Zn and Hg in their tissues linked to their nutritional status (emaciation). The question arises regarding a potential role of metallothioneins (MTs) with regard to these high metal levels. In the present study, metallothionein detection and associated Zn, Cd, Cu and Hg concentrations were investigated in the liver and kidney of 14 harbour porpoises collected along the Belgian coast. Results Metallothioneins seemed to play a key role in essential metal homeostasis, as they were shown to bind 50% of the total hepatic Zn and 36% of the total hepatic Cu concentrations. Renal MTs also participated in Cd detoxification, as they were shown to bind 56% of the total renal Cd. Hg was mainly found in the insoluble fraction of both liver and kidney. Concomitant increases in total Zn concentration and Zn bound to MTs were observed in the liver, whereas Zn concentration bound to high molecular weight proteins remained constant. Cu, Zn and Cd were accumulated preferentially in the MT fraction and their content in this fraction increased with the amount in the hepatocytosol. Conclusion MTs have a key role in Zn and Cu homeostasis in harbour porpoises. We demonstrated that increasing hepatic Zn concentration led to an increase in Zn linked to MTs, suggesting that these small proteins take over the Zn overload linked to the poor body condition of debilitated harbour porpoises. |
format |
Article in Journal/Newspaper |
author |
Jauniaux Thierry De Groof Arnaud Das Krishna Bouquegneau Jean-Marie |
author_facet |
Jauniaux Thierry De Groof Arnaud Das Krishna Bouquegneau Jean-Marie |
author_sort |
Jauniaux Thierry |
title |
Zn, Cu, Cd and Hg binding to metallothioneins in harbour porpoises Phocoena phocoena from the southern North Sea |
title_short |
Zn, Cu, Cd and Hg binding to metallothioneins in harbour porpoises Phocoena phocoena from the southern North Sea |
title_full |
Zn, Cu, Cd and Hg binding to metallothioneins in harbour porpoises Phocoena phocoena from the southern North Sea |
title_fullStr |
Zn, Cu, Cd and Hg binding to metallothioneins in harbour porpoises Phocoena phocoena from the southern North Sea |
title_full_unstemmed |
Zn, Cu, Cd and Hg binding to metallothioneins in harbour porpoises Phocoena phocoena from the southern North Sea |
title_sort |
zn, cu, cd and hg binding to metallothioneins in harbour porpoises phocoena phocoena from the southern north sea |
publisher |
BMC |
publishDate |
2006 |
url |
https://doi.org/10.1186/1472-6785-6-2 https://doaj.org/article/4677c191d20441adb6f961b471b7df5d |
genre |
Phocoena phocoena |
genre_facet |
Phocoena phocoena |
op_source |
BMC Ecology, Vol 6, Iss 1, p 2 (2006) |
op_relation |
http://www.biomedcentral.com/1472-6785/6/2 https://doaj.org/toc/1472-6785 doi:10.1186/1472-6785-6-2 1472-6785 https://doaj.org/article/4677c191d20441adb6f961b471b7df5d |
op_doi |
https://doi.org/10.1186/1472-6785-6-2 |
container_title |
BMC Ecology |
container_volume |
6 |
container_issue |
1 |
container_start_page |
2 |
_version_ |
1766167899055063040 |