Antibody trapping: A novel mechanism of parasite immune evasion by the trematode Echinostoma caproni.
Helminth infections are among the most prevalent neglected tropical diseases, causing an enormous impact in global health and the socioeconomic growth of developing countries. In this context, the study of helminth biology, with emphasis on host-parasite interactions, appears as a promising approach...
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ftdoajarticles:oai:doaj.org/article:4656b0b2125d4d36b0e80fc2be7a4b54 2023-05-15T15:13:55+02:00 Antibody trapping: A novel mechanism of parasite immune evasion by the trematode Echinostoma caproni. Alba Cortés Javier Sotillo Carla Muñoz-Antolí Javier Molina-Durán J Guillermo Esteban Rafael Toledo 2017-07-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005773 https://doaj.org/article/4656b0b2125d4d36b0e80fc2be7a4b54 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5531663?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005773 https://doaj.org/article/4656b0b2125d4d36b0e80fc2be7a4b54 PLoS Neglected Tropical Diseases, Vol 11, Iss 7, p e0005773 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005773 2022-12-31T14:03:43Z Helminth infections are among the most prevalent neglected tropical diseases, causing an enormous impact in global health and the socioeconomic growth of developing countries. In this context, the study of helminth biology, with emphasis on host-parasite interactions, appears as a promising approach for developing new tools to prevent and control these infections.The role that antibody responses have on helminth infections is still not well understood. To go in depth into this issue, work on the intestinal helminth Echinostoma caproni (Trematoda: Echinostomatidae) has been undertaken. Adult parasites were recovered from infected mice and cultured in vitro. Double indirect immunofluorescence at increasing culture times was done to show that in vivo-bound surface antibodies become trapped within a layer of excretory/secretory products that covers the parasite. Entrapped antibodies are then degraded by parasite-derived proteases, since protease inhibitors prevent for antibody loss in culture. Electron microscopy and immunogold-labelling of secreted proteins provide evidence that this mechanism is consistent with tegument dynamics and ultrastructure, hence it is feasible to occur in vivo. Secretory vesicles discharge their content to the outside and released products are deposited over the parasite surface enabling antibody trapping.At the site of infection, both parasite secretion and antibody binding occur simultaneously and constantly. The continuous entrapment of bound antibodies with newly secreted products may serve to minimize the deleterious effects of the antibody-mediated attack. This mechanism of immune evasion may aid to understand the limited effect that antibody responses have in helminth infections, and may contribute to the basis for vaccine development against these highly prevalent diseases. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 7 e0005773 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Alba Cortés Javier Sotillo Carla Muñoz-Antolí Javier Molina-Durán J Guillermo Esteban Rafael Toledo Antibody trapping: A novel mechanism of parasite immune evasion by the trematode Echinostoma caproni. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Helminth infections are among the most prevalent neglected tropical diseases, causing an enormous impact in global health and the socioeconomic growth of developing countries. In this context, the study of helminth biology, with emphasis on host-parasite interactions, appears as a promising approach for developing new tools to prevent and control these infections.The role that antibody responses have on helminth infections is still not well understood. To go in depth into this issue, work on the intestinal helminth Echinostoma caproni (Trematoda: Echinostomatidae) has been undertaken. Adult parasites were recovered from infected mice and cultured in vitro. Double indirect immunofluorescence at increasing culture times was done to show that in vivo-bound surface antibodies become trapped within a layer of excretory/secretory products that covers the parasite. Entrapped antibodies are then degraded by parasite-derived proteases, since protease inhibitors prevent for antibody loss in culture. Electron microscopy and immunogold-labelling of secreted proteins provide evidence that this mechanism is consistent with tegument dynamics and ultrastructure, hence it is feasible to occur in vivo. Secretory vesicles discharge their content to the outside and released products are deposited over the parasite surface enabling antibody trapping.At the site of infection, both parasite secretion and antibody binding occur simultaneously and constantly. The continuous entrapment of bound antibodies with newly secreted products may serve to minimize the deleterious effects of the antibody-mediated attack. This mechanism of immune evasion may aid to understand the limited effect that antibody responses have in helminth infections, and may contribute to the basis for vaccine development against these highly prevalent diseases. |
format |
Article in Journal/Newspaper |
author |
Alba Cortés Javier Sotillo Carla Muñoz-Antolí Javier Molina-Durán J Guillermo Esteban Rafael Toledo |
author_facet |
Alba Cortés Javier Sotillo Carla Muñoz-Antolí Javier Molina-Durán J Guillermo Esteban Rafael Toledo |
author_sort |
Alba Cortés |
title |
Antibody trapping: A novel mechanism of parasite immune evasion by the trematode Echinostoma caproni. |
title_short |
Antibody trapping: A novel mechanism of parasite immune evasion by the trematode Echinostoma caproni. |
title_full |
Antibody trapping: A novel mechanism of parasite immune evasion by the trematode Echinostoma caproni. |
title_fullStr |
Antibody trapping: A novel mechanism of parasite immune evasion by the trematode Echinostoma caproni. |
title_full_unstemmed |
Antibody trapping: A novel mechanism of parasite immune evasion by the trematode Echinostoma caproni. |
title_sort |
antibody trapping: a novel mechanism of parasite immune evasion by the trematode echinostoma caproni. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0005773 https://doaj.org/article/4656b0b2125d4d36b0e80fc2be7a4b54 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 7, p e0005773 (2017) |
op_relation |
http://europepmc.org/articles/PMC5531663?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005773 https://doaj.org/article/4656b0b2125d4d36b0e80fc2be7a4b54 |
op_doi |
https://doi.org/10.1371/journal.pntd.0005773 |
container_title |
PLOS Neglected Tropical Diseases |
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11 |
container_issue |
7 |
container_start_page |
e0005773 |
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1766344425863118848 |