Genomic Characteristics and Functional Analysis of Brucella sp. Strain WY7 Isolated from Antarctic Krill
Antarctic krill ( Euphausia superba ) is a key species of the Antarctic ecosystem whose unique ecological status and great development potential have attracted extensive attention. However, the genomic characteristics and potential biological functions of the symbiotic microorganisms of Antarctic kr...
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ftdoajarticles:oai:doaj.org/article:4655d28adc7547abafe893c9120852ae 2023-10-29T02:32:28+01:00 Genomic Characteristics and Functional Analysis of Brucella sp. Strain WY7 Isolated from Antarctic Krill Zhengqi Feng Yuanyuan Wang Lingbo Ma Shanzi Huang Lumin Wang Jianguo He Changjun Guo 2023-09-01T00:00:00Z https://doi.org/10.3390/microorganisms11092281 https://doaj.org/article/4655d28adc7547abafe893c9120852ae EN eng MDPI AG https://www.mdpi.com/2076-2607/11/9/2281 https://doaj.org/toc/2076-2607 doi:10.3390/microorganisms11092281 2076-2607 https://doaj.org/article/4655d28adc7547abafe893c9120852ae Microorganisms, Vol 11, Iss 2281, p 2281 (2023) Antarctic krill microorganism extremophiles Brucella whole genome Biology (General) QH301-705.5 article 2023 ftdoajarticles https://doi.org/10.3390/microorganisms11092281 2023-10-01T00:37:35Z Antarctic krill ( Euphausia superba ) is a key species of the Antarctic ecosystem whose unique ecological status and great development potential have attracted extensive attention. However, the genomic characteristics and potential biological functions of the symbiotic microorganisms of Antarctic krill remain unknown. In this study, we cultured and identified a strain of Brucella sp. WY7 from Antarctic krill using whole-genome sequencing and assembly, functional annotation, and comparative genomics analysis. First, based on 16S rDNA sequence alignment and phylogenetic tree analysis, we identified strain WY7 as Brucella . The assembled genome of strain WY7 revealed that it has two chromosomes and a plasmid, with a total genome length of 4,698,850 bp and an average G + C content of 57.18%. The DNA—DNA hybridization value and average nucleotide identity value of strain WY7 and Brucella anthropi ATCC ® 49188 TM , a type strain isolated from human clinical specimens, were 94.8% and 99.07%, respectively, indicating that strain WY7 is closely related to Brucella anthropi . Genomic island prediction showed that the strain has 60 genomic islands, which may produce HigB and VapC toxins. AntiSMASH analysis results showed that strain WY7 might produce many secondary metabolites, such as terpenes, siderophores and ectoine. Moreover, the genome contains genes involved in the degradation of aromatic compounds, suggesting that strain WY7 can use aromatic compounds in its metabolism. Our work will help to understand the genomic characteristics and metabolic potential of bacterial strains isolated from Antarctic krill, thereby revealing their roles in Antarctic krill and marine ecosystems. Article in Journal/Newspaper Antarc* Antarctic Antarctic Krill Euphausia superba Directory of Open Access Journals: DOAJ Articles Microorganisms 11 9 2281 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Antarctic krill microorganism extremophiles Brucella whole genome Biology (General) QH301-705.5 |
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Antarctic krill microorganism extremophiles Brucella whole genome Biology (General) QH301-705.5 Zhengqi Feng Yuanyuan Wang Lingbo Ma Shanzi Huang Lumin Wang Jianguo He Changjun Guo Genomic Characteristics and Functional Analysis of Brucella sp. Strain WY7 Isolated from Antarctic Krill |
topic_facet |
Antarctic krill microorganism extremophiles Brucella whole genome Biology (General) QH301-705.5 |
description |
Antarctic krill ( Euphausia superba ) is a key species of the Antarctic ecosystem whose unique ecological status and great development potential have attracted extensive attention. However, the genomic characteristics and potential biological functions of the symbiotic microorganisms of Antarctic krill remain unknown. In this study, we cultured and identified a strain of Brucella sp. WY7 from Antarctic krill using whole-genome sequencing and assembly, functional annotation, and comparative genomics analysis. First, based on 16S rDNA sequence alignment and phylogenetic tree analysis, we identified strain WY7 as Brucella . The assembled genome of strain WY7 revealed that it has two chromosomes and a plasmid, with a total genome length of 4,698,850 bp and an average G + C content of 57.18%. The DNA—DNA hybridization value and average nucleotide identity value of strain WY7 and Brucella anthropi ATCC ® 49188 TM , a type strain isolated from human clinical specimens, were 94.8% and 99.07%, respectively, indicating that strain WY7 is closely related to Brucella anthropi . Genomic island prediction showed that the strain has 60 genomic islands, which may produce HigB and VapC toxins. AntiSMASH analysis results showed that strain WY7 might produce many secondary metabolites, such as terpenes, siderophores and ectoine. Moreover, the genome contains genes involved in the degradation of aromatic compounds, suggesting that strain WY7 can use aromatic compounds in its metabolism. Our work will help to understand the genomic characteristics and metabolic potential of bacterial strains isolated from Antarctic krill, thereby revealing their roles in Antarctic krill and marine ecosystems. |
format |
Article in Journal/Newspaper |
author |
Zhengqi Feng Yuanyuan Wang Lingbo Ma Shanzi Huang Lumin Wang Jianguo He Changjun Guo |
author_facet |
Zhengqi Feng Yuanyuan Wang Lingbo Ma Shanzi Huang Lumin Wang Jianguo He Changjun Guo |
author_sort |
Zhengqi Feng |
title |
Genomic Characteristics and Functional Analysis of Brucella sp. Strain WY7 Isolated from Antarctic Krill |
title_short |
Genomic Characteristics and Functional Analysis of Brucella sp. Strain WY7 Isolated from Antarctic Krill |
title_full |
Genomic Characteristics and Functional Analysis of Brucella sp. Strain WY7 Isolated from Antarctic Krill |
title_fullStr |
Genomic Characteristics and Functional Analysis of Brucella sp. Strain WY7 Isolated from Antarctic Krill |
title_full_unstemmed |
Genomic Characteristics and Functional Analysis of Brucella sp. Strain WY7 Isolated from Antarctic Krill |
title_sort |
genomic characteristics and functional analysis of brucella sp. strain wy7 isolated from antarctic krill |
publisher |
MDPI AG |
publishDate |
2023 |
url |
https://doi.org/10.3390/microorganisms11092281 https://doaj.org/article/4655d28adc7547abafe893c9120852ae |
genre |
Antarc* Antarctic Antarctic Krill Euphausia superba |
genre_facet |
Antarc* Antarctic Antarctic Krill Euphausia superba |
op_source |
Microorganisms, Vol 11, Iss 2281, p 2281 (2023) |
op_relation |
https://www.mdpi.com/2076-2607/11/9/2281 https://doaj.org/toc/2076-2607 doi:10.3390/microorganisms11092281 2076-2607 https://doaj.org/article/4655d28adc7547abafe893c9120852ae |
op_doi |
https://doi.org/10.3390/microorganisms11092281 |
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Microorganisms |
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11 |
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9 |
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2281 |
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1781053911400972288 |