In vivo sedative and muscle relaxants activity of Diospyros lotus L

• Published in: Asian Pacific Journal of Tropical Biomedicine
• Main Authors: Abdur Rauf, Ghias Uddin, Bina Shaheen Siddiqui, Haroon Khan
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wolters Kluwer Medknow Publications 2015
• Subjects: Diospyros lotus; Ebenaceae; Sedative; Muscle relaxants activity; Arctic medicine. Tropical medicine; RC955-962; Biology (General); QH301-705.5; Arctic
• Online Access: https://doi.org/10.1016/S2221-1691(15)30345-2; https://doaj.org/article/4639ff8e8ee74019b751f48655937a0e

Objective: To evaluate the sedative effect of Diospyros lotus L (D. lotus) extract in mice using the open field and Rota rod tests. Methods: For the sedative and muscle relaxants activities of extract/fractions of the plant, in-vivo open field and phenobarbitone-induced sleeping time were used, while the Roda rod test was employed in animals for the assessment of muscle relaxant activity. Results: Results from this investigation revealed that the extracts of D. lotus have exhibited significant sedative effect in mice (45.98%) at 100 mg/kg i.p. When the extract was partitioned with different solvents, the n-hexane fraction was inactive whereas the chloroform fraction was the most active with 82.67% sedative effect at 50 and 100 mg/kg i.p. On the other hand, the ethyl acetate and n-butanol fractions displayed significant sedative effects (55.65% and 40.87%, respectively) at 100 mg/kg i.p. Among the tested extract/fractions, only chloroform and ethyl acetate fractions showed significant (P < 0.05) muscle relaxant activity in the Rota rod test. Conclusions: In short, our study provided scientific background to the traditional uses of D. lotus as sedative.