European Aedes albopictus and Culex pipiens Are Competent Vectors for Japanese Encephalitis Virus.
BACKGROUND:Japanese encephalitis virus (JEV) is the causative agent of Japanese encephalitis, the leading cause of viral encephalitis in Asia. JEV transmission cycle involves mosquitoes and vertebrate hosts. The detection of JEV RNA in a pool of Culex pipiens caught in 2010 in Italy raised the conce...
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ftdoajarticles:oai:doaj.org/article:4634721c946e4014b7a96b876b52239e 2023-05-15T15:13:49+02:00 European Aedes albopictus and Culex pipiens Are Competent Vectors for Japanese Encephalitis Virus. Mélissanne de Wispelaere Philippe Desprès Valérie Choumet 2017-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005294 https://doaj.org/article/4634721c946e4014b7a96b876b52239e EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5268654?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005294 https://doaj.org/article/4634721c946e4014b7a96b876b52239e PLoS Neglected Tropical Diseases, Vol 11, Iss 1, p e0005294 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005294 2022-12-31T16:33:41Z BACKGROUND:Japanese encephalitis virus (JEV) is the causative agent of Japanese encephalitis, the leading cause of viral encephalitis in Asia. JEV transmission cycle involves mosquitoes and vertebrate hosts. The detection of JEV RNA in a pool of Culex pipiens caught in 2010 in Italy raised the concern of a putative emergence of the virus in Europe. We aimed to study the vector competence of European mosquito populations, such as Cx. pipiens and Aedes albopictus for JEV genotypes 3 and 5. FINDINGS:After oral feeding on an infectious blood meal, mosquitoes were dissected at various times post-virus exposure. We found that the peak for JEV infection and transmission was between 11 and 13 days post-virus exposure. We observed a faster dissemination of both JEV genotypes in Ae. albopictus mosquitoes, when compared with Cx. pipiens mosquitoes. We also dissected salivary glands and collected saliva from infected mosquitoes and showed that Ae. albopictus mosquitoes transmitted JEV earlier than Cx. pipiens. The virus collected from Ae. albopictus and Cx. pipiens saliva was competent at causing pathogenesis in a mouse model for JEV infection. Using this model, we found that mosquito saliva or salivary glands did not enhance the severity of the disease. CONCLUSIONS:In this study, we demonstrated that European populations of Ae. albopictus and Cx. pipiens were efficient vectors for JEV transmission. Susceptible vertebrate species that develop high viremia are an obligatory part of the JEV transmission cycle. This study highlights the need to investigate the susceptibility of potential JEV reservoir hosts in Europe, notably amongst swine populations and local water birds. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 1 e0005294 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Mélissanne de Wispelaere Philippe Desprès Valérie Choumet European Aedes albopictus and Culex pipiens Are Competent Vectors for Japanese Encephalitis Virus. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:Japanese encephalitis virus (JEV) is the causative agent of Japanese encephalitis, the leading cause of viral encephalitis in Asia. JEV transmission cycle involves mosquitoes and vertebrate hosts. The detection of JEV RNA in a pool of Culex pipiens caught in 2010 in Italy raised the concern of a putative emergence of the virus in Europe. We aimed to study the vector competence of European mosquito populations, such as Cx. pipiens and Aedes albopictus for JEV genotypes 3 and 5. FINDINGS:After oral feeding on an infectious blood meal, mosquitoes were dissected at various times post-virus exposure. We found that the peak for JEV infection and transmission was between 11 and 13 days post-virus exposure. We observed a faster dissemination of both JEV genotypes in Ae. albopictus mosquitoes, when compared with Cx. pipiens mosquitoes. We also dissected salivary glands and collected saliva from infected mosquitoes and showed that Ae. albopictus mosquitoes transmitted JEV earlier than Cx. pipiens. The virus collected from Ae. albopictus and Cx. pipiens saliva was competent at causing pathogenesis in a mouse model for JEV infection. Using this model, we found that mosquito saliva or salivary glands did not enhance the severity of the disease. CONCLUSIONS:In this study, we demonstrated that European populations of Ae. albopictus and Cx. pipiens were efficient vectors for JEV transmission. Susceptible vertebrate species that develop high viremia are an obligatory part of the JEV transmission cycle. This study highlights the need to investigate the susceptibility of potential JEV reservoir hosts in Europe, notably amongst swine populations and local water birds. |
format |
Article in Journal/Newspaper |
author |
Mélissanne de Wispelaere Philippe Desprès Valérie Choumet |
author_facet |
Mélissanne de Wispelaere Philippe Desprès Valérie Choumet |
author_sort |
Mélissanne de Wispelaere |
title |
European Aedes albopictus and Culex pipiens Are Competent Vectors for Japanese Encephalitis Virus. |
title_short |
European Aedes albopictus and Culex pipiens Are Competent Vectors for Japanese Encephalitis Virus. |
title_full |
European Aedes albopictus and Culex pipiens Are Competent Vectors for Japanese Encephalitis Virus. |
title_fullStr |
European Aedes albopictus and Culex pipiens Are Competent Vectors for Japanese Encephalitis Virus. |
title_full_unstemmed |
European Aedes albopictus and Culex pipiens Are Competent Vectors for Japanese Encephalitis Virus. |
title_sort |
european aedes albopictus and culex pipiens are competent vectors for japanese encephalitis virus. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0005294 https://doaj.org/article/4634721c946e4014b7a96b876b52239e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 1, p e0005294 (2017) |
op_relation |
http://europepmc.org/articles/PMC5268654?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005294 https://doaj.org/article/4634721c946e4014b7a96b876b52239e |
op_doi |
https://doi.org/10.1371/journal.pntd.0005294 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
11 |
container_issue |
1 |
container_start_page |
e0005294 |
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1766344337083334656 |