RNAi Silencing of the Biomineralization Gene Perlucin Impairs Oyster Ability to Cope with Ocean Acidification
Calcifying marine organisms, including the eastern oyster ( Crassostrea virginica ), are vulnerable to ocean acidification (OA) because it is more difficult to precipitate calcium carbonate (CaCO 3 ). Previous investigations of the molecular mechanisms associated with resilience to OA in C. virginic...
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ftdoajarticles:oai:doaj.org/article:460a0237b4f64c0ca3c1befd911f2c6c 2023-05-15T17:49:54+02:00 RNAi Silencing of the Biomineralization Gene Perlucin Impairs Oyster Ability to Cope with Ocean Acidification Caroline Schwaner Emmanuelle Pales Espinosa Bassem Allam 2023-02-01T00:00:00Z https://doi.org/10.3390/ijms24043661 https://doaj.org/article/460a0237b4f64c0ca3c1befd911f2c6c EN eng MDPI AG https://www.mdpi.com/1422-0067/24/4/3661 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms24043661 1422-0067 1661-6596 https://doaj.org/article/460a0237b4f64c0ca3c1befd911f2c6c International Journal of Molecular Sciences, Vol 24, Iss 3661, p 3661 (2023) gene silencing perlucin oyster bivalve ocean acidification Biology (General) QH301-705.5 Chemistry QD1-999 article 2023 ftdoajarticles https://doi.org/10.3390/ijms24043661 2023-02-26T01:29:53Z Calcifying marine organisms, including the eastern oyster ( Crassostrea virginica ), are vulnerable to ocean acidification (OA) because it is more difficult to precipitate calcium carbonate (CaCO 3 ). Previous investigations of the molecular mechanisms associated with resilience to OA in C. virginica demonstrated significant differences in single nucleotide polymorphism and gene expression profiles among oysters reared under ambient and OA conditions. Converged evidence generated by both of these approaches highlighted the role of genes related to biomineralization, including perlucins. Here, gene silencing via RNA interference (RNAi) was used to evaluate the protective role of a perlucin gene under OA stress. Larvae were exposed to short dicer-substrate small interfering RNA (DsiRNA-perlucin) to silence the target gene or to one of two control treatments (control DsiRNA or seawater) before cultivation under OA (pH ~7.3) or ambient (pH ~8.2) conditions. Two transfection experiments were performed in parallel, one during fertilization and one during early larval development (6 h post-fertilization), before larval viability, size, development, and shell mineralization were monitored. Silenced oysters under acidification stress were the smallest, had shell abnormalities, and had significantly reduced shell mineralization, thereby suggesting that perlucin significantly helps larvae mitigate the effects of OA. Article in Journal/Newspaper Ocean acidification Directory of Open Access Journals: DOAJ Articles International Journal of Molecular Sciences 24 4 3661 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
gene silencing perlucin oyster bivalve ocean acidification Biology (General) QH301-705.5 Chemistry QD1-999 |
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gene silencing perlucin oyster bivalve ocean acidification Biology (General) QH301-705.5 Chemistry QD1-999 Caroline Schwaner Emmanuelle Pales Espinosa Bassem Allam RNAi Silencing of the Biomineralization Gene Perlucin Impairs Oyster Ability to Cope with Ocean Acidification |
topic_facet |
gene silencing perlucin oyster bivalve ocean acidification Biology (General) QH301-705.5 Chemistry QD1-999 |
description |
Calcifying marine organisms, including the eastern oyster ( Crassostrea virginica ), are vulnerable to ocean acidification (OA) because it is more difficult to precipitate calcium carbonate (CaCO 3 ). Previous investigations of the molecular mechanisms associated with resilience to OA in C. virginica demonstrated significant differences in single nucleotide polymorphism and gene expression profiles among oysters reared under ambient and OA conditions. Converged evidence generated by both of these approaches highlighted the role of genes related to biomineralization, including perlucins. Here, gene silencing via RNA interference (RNAi) was used to evaluate the protective role of a perlucin gene under OA stress. Larvae were exposed to short dicer-substrate small interfering RNA (DsiRNA-perlucin) to silence the target gene or to one of two control treatments (control DsiRNA or seawater) before cultivation under OA (pH ~7.3) or ambient (pH ~8.2) conditions. Two transfection experiments were performed in parallel, one during fertilization and one during early larval development (6 h post-fertilization), before larval viability, size, development, and shell mineralization were monitored. Silenced oysters under acidification stress were the smallest, had shell abnormalities, and had significantly reduced shell mineralization, thereby suggesting that perlucin significantly helps larvae mitigate the effects of OA. |
format |
Article in Journal/Newspaper |
author |
Caroline Schwaner Emmanuelle Pales Espinosa Bassem Allam |
author_facet |
Caroline Schwaner Emmanuelle Pales Espinosa Bassem Allam |
author_sort |
Caroline Schwaner |
title |
RNAi Silencing of the Biomineralization Gene Perlucin Impairs Oyster Ability to Cope with Ocean Acidification |
title_short |
RNAi Silencing of the Biomineralization Gene Perlucin Impairs Oyster Ability to Cope with Ocean Acidification |
title_full |
RNAi Silencing of the Biomineralization Gene Perlucin Impairs Oyster Ability to Cope with Ocean Acidification |
title_fullStr |
RNAi Silencing of the Biomineralization Gene Perlucin Impairs Oyster Ability to Cope with Ocean Acidification |
title_full_unstemmed |
RNAi Silencing of the Biomineralization Gene Perlucin Impairs Oyster Ability to Cope with Ocean Acidification |
title_sort |
rnai silencing of the biomineralization gene perlucin impairs oyster ability to cope with ocean acidification |
publisher |
MDPI AG |
publishDate |
2023 |
url |
https://doi.org/10.3390/ijms24043661 https://doaj.org/article/460a0237b4f64c0ca3c1befd911f2c6c |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
International Journal of Molecular Sciences, Vol 24, Iss 3661, p 3661 (2023) |
op_relation |
https://www.mdpi.com/1422-0067/24/4/3661 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms24043661 1422-0067 1661-6596 https://doaj.org/article/460a0237b4f64c0ca3c1befd911f2c6c |
op_doi |
https://doi.org/10.3390/ijms24043661 |
container_title |
International Journal of Molecular Sciences |
container_volume |
24 |
container_issue |
4 |
container_start_page |
3661 |
_version_ |
1766156421603262464 |