Human Neutrophil Peptide 1 as immunotherapeutic agent against Leishmania infected BALB/c mice.
Human Neutrophil Peptide 1 (HNP1) produced by neutrophils, is a well-known antimicrobial peptide which plays a role both in innate as well as in adaptive immunity and is under intensive investigation as a potential therapeutic agent. Previous in vitro experiments have indicated the leishmaniacidal e...
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ftdoajarticles:oai:doaj.org/article:45b0d5df627b410e99e7c813a4dc4688 2023-05-15T15:10:44+02:00 Human Neutrophil Peptide 1 as immunotherapeutic agent against Leishmania infected BALB/c mice. Zahra Abdossamadi Negar Seyed Farnaz Zahedifard Tahereh Taheri Yasaman Taslimi Hossein Montakhab-Yeganeh Alireza Badirzadeh Mohammad Vasei Safoora Gharibzadeh Sima Rafati 2017-12-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0006123 https://doaj.org/article/45b0d5df627b410e99e7c813a4dc4688 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5749894?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006123 https://doaj.org/article/45b0d5df627b410e99e7c813a4dc4688 PLoS Neglected Tropical Diseases, Vol 11, Iss 12, p e0006123 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0006123 2022-12-30T22:27:34Z Human Neutrophil Peptide 1 (HNP1) produced by neutrophils, is a well-known antimicrobial peptide which plays a role both in innate as well as in adaptive immunity and is under intensive investigation as a potential therapeutic agent. Previous in vitro experiments have indicated the leishmaniacidal effect of recombinant HNP1 on Leishmania major (L. major) promastigotes and amastigotes. In the current study, we further extended the idea to explore the remedial effect of HNP1 in the two modalities of peptide therapy (folded HNP1) and gene therapy in L. major infected BALB/c mice. To this end, mice in five different groups received synthetic folded HNP1 (G1), pcDNA-HNP1-EGFP (G2), pcDNA-EGFP (G3), Amphotericin B (G4) and PBS (G5), which was started three weeks after infection for three consecutive weeks. Footpad swelling was monitored weekly and a day after the therapy ended, IFN-γ, IL-4, IL-10, IL-6 and nitric oxide produced by splenocytes were analyzed together with the parasite load in draining lymph nodes. Arginase activity and dermal histopathological changes were also analyzed in the infected footpads. We demonstrated that both therapeutic approaches effectively induced Th1 polarization and restricted parasite burden. It can control disease progression in contrast to non-treated groups. However, pcDNA-HNP1-EGFP is more promising in respect to parasite control than folded HNP1, but less effective than AmB treatment. We concluded with the call for a future approach, that is, a DNA-based expression of HNP1 combined with AmB as it can improve the leishmaniacidal efficacy. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 12 e0006123 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Zahra Abdossamadi Negar Seyed Farnaz Zahedifard Tahereh Taheri Yasaman Taslimi Hossein Montakhab-Yeganeh Alireza Badirzadeh Mohammad Vasei Safoora Gharibzadeh Sima Rafati Human Neutrophil Peptide 1 as immunotherapeutic agent against Leishmania infected BALB/c mice. |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Human Neutrophil Peptide 1 (HNP1) produced by neutrophils, is a well-known antimicrobial peptide which plays a role both in innate as well as in adaptive immunity and is under intensive investigation as a potential therapeutic agent. Previous in vitro experiments have indicated the leishmaniacidal effect of recombinant HNP1 on Leishmania major (L. major) promastigotes and amastigotes. In the current study, we further extended the idea to explore the remedial effect of HNP1 in the two modalities of peptide therapy (folded HNP1) and gene therapy in L. major infected BALB/c mice. To this end, mice in five different groups received synthetic folded HNP1 (G1), pcDNA-HNP1-EGFP (G2), pcDNA-EGFP (G3), Amphotericin B (G4) and PBS (G5), which was started three weeks after infection for three consecutive weeks. Footpad swelling was monitored weekly and a day after the therapy ended, IFN-γ, IL-4, IL-10, IL-6 and nitric oxide produced by splenocytes were analyzed together with the parasite load in draining lymph nodes. Arginase activity and dermal histopathological changes were also analyzed in the infected footpads. We demonstrated that both therapeutic approaches effectively induced Th1 polarization and restricted parasite burden. It can control disease progression in contrast to non-treated groups. However, pcDNA-HNP1-EGFP is more promising in respect to parasite control than folded HNP1, but less effective than AmB treatment. We concluded with the call for a future approach, that is, a DNA-based expression of HNP1 combined with AmB as it can improve the leishmaniacidal efficacy. |
format |
Article in Journal/Newspaper |
author |
Zahra Abdossamadi Negar Seyed Farnaz Zahedifard Tahereh Taheri Yasaman Taslimi Hossein Montakhab-Yeganeh Alireza Badirzadeh Mohammad Vasei Safoora Gharibzadeh Sima Rafati |
author_facet |
Zahra Abdossamadi Negar Seyed Farnaz Zahedifard Tahereh Taheri Yasaman Taslimi Hossein Montakhab-Yeganeh Alireza Badirzadeh Mohammad Vasei Safoora Gharibzadeh Sima Rafati |
author_sort |
Zahra Abdossamadi |
title |
Human Neutrophil Peptide 1 as immunotherapeutic agent against Leishmania infected BALB/c mice. |
title_short |
Human Neutrophil Peptide 1 as immunotherapeutic agent against Leishmania infected BALB/c mice. |
title_full |
Human Neutrophil Peptide 1 as immunotherapeutic agent against Leishmania infected BALB/c mice. |
title_fullStr |
Human Neutrophil Peptide 1 as immunotherapeutic agent against Leishmania infected BALB/c mice. |
title_full_unstemmed |
Human Neutrophil Peptide 1 as immunotherapeutic agent against Leishmania infected BALB/c mice. |
title_sort |
human neutrophil peptide 1 as immunotherapeutic agent against leishmania infected balb/c mice. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0006123 https://doaj.org/article/45b0d5df627b410e99e7c813a4dc4688 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 12, p e0006123 (2017) |
op_relation |
http://europepmc.org/articles/PMC5749894?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006123 https://doaj.org/article/45b0d5df627b410e99e7c813a4dc4688 |
op_doi |
https://doi.org/10.1371/journal.pntd.0006123 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
11 |
container_issue |
12 |
container_start_page |
e0006123 |
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1766341701137334272 |