Genetic diversity and antimicrobial resistance of enterococcal isolates from Southern region of Brazil
In the present study, a total of 455 enterococcal isolates, recovered from patients living in the city of Porto Alegre, State of Rio Grande do Sul, Brazil, during the period from July 1996 to June 1997, were identified to the species level by conventional biochemical and microbiological tests, and a...
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ftdoajarticles:oai:doaj.org/article:455dc501a6d5409bbd63ed4536b377f5 2024-09-09T19:25:59+00:00 Genetic diversity and antimicrobial resistance of enterococcal isolates from Southern region of Brazil Pedro Alves d'Azevedo Cícero Armídio Gomes Dias Lúcia Martins Teixeira 2006-02-01T00:00:00Z https://doaj.org/article/455dc501a6d5409bbd63ed4536b377f5 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652006000100003&tlng=en https://doaj.org/toc/1678-9946 1678-9946 https://doaj.org/article/455dc501a6d5409bbd63ed4536b377f5 Revista do Instituto de Medicina Tropical de São Paulo, Vol 48, Iss 1, Pp 11-16 (2006) Enterococci High level resistance aminoglycosides Genetic diversity Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2006 ftdoajarticles 2024-08-05T17:49:31Z In the present study, a total of 455 enterococcal isolates, recovered from patients living in the city of Porto Alegre, State of Rio Grande do Sul, Brazil, during the period from July 1996 to June 1997, were identified to the species level by conventional biochemical and microbiological tests, and assayed for their susceptibilities to antimicrobial agents. The genetic diversity of antimicrobial resistant strains was evaluated by pulsed-field gel electrophoresis (PFGE) analysis of SmaI restricted chromosomal DNA. The most frequent species was Enterococcus faecalis (92.8%). Other species identified were: E. faecium (2.9%), E. gallinarum (1.5%), E. avium (1.1%), E. hirae (0.7%), E. casseliflavus (0.4%), E. durans (0.4%) and E. raffinosus (0.2%). The overall prevalence of isolates with high-level resistance (HLR) to aminoglycosides was 37.8%. HLR to gentamicin was found in 24.8%. No strains with acquired resistance to vancomycin were found. PFGE analysis showed the predominance of clonal group A, comprising strains isolated from different clinical specimens obtained from patients in three hospitals. These results suggest intra and inter-hospital dissemination of one predominant clonal group of E. faecalis isolates with HLR to gentamicin in the hospitals included in this study. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
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topic |
Enterococci High level resistance aminoglycosides Genetic diversity Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Enterococci High level resistance aminoglycosides Genetic diversity Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Pedro Alves d'Azevedo Cícero Armídio Gomes Dias Lúcia Martins Teixeira Genetic diversity and antimicrobial resistance of enterococcal isolates from Southern region of Brazil |
topic_facet |
Enterococci High level resistance aminoglycosides Genetic diversity Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
In the present study, a total of 455 enterococcal isolates, recovered from patients living in the city of Porto Alegre, State of Rio Grande do Sul, Brazil, during the period from July 1996 to June 1997, were identified to the species level by conventional biochemical and microbiological tests, and assayed for their susceptibilities to antimicrobial agents. The genetic diversity of antimicrobial resistant strains was evaluated by pulsed-field gel electrophoresis (PFGE) analysis of SmaI restricted chromosomal DNA. The most frequent species was Enterococcus faecalis (92.8%). Other species identified were: E. faecium (2.9%), E. gallinarum (1.5%), E. avium (1.1%), E. hirae (0.7%), E. casseliflavus (0.4%), E. durans (0.4%) and E. raffinosus (0.2%). The overall prevalence of isolates with high-level resistance (HLR) to aminoglycosides was 37.8%. HLR to gentamicin was found in 24.8%. No strains with acquired resistance to vancomycin were found. PFGE analysis showed the predominance of clonal group A, comprising strains isolated from different clinical specimens obtained from patients in three hospitals. These results suggest intra and inter-hospital dissemination of one predominant clonal group of E. faecalis isolates with HLR to gentamicin in the hospitals included in this study. |
format |
Article in Journal/Newspaper |
author |
Pedro Alves d'Azevedo Cícero Armídio Gomes Dias Lúcia Martins Teixeira |
author_facet |
Pedro Alves d'Azevedo Cícero Armídio Gomes Dias Lúcia Martins Teixeira |
author_sort |
Pedro Alves d'Azevedo |
title |
Genetic diversity and antimicrobial resistance of enterococcal isolates from Southern region of Brazil |
title_short |
Genetic diversity and antimicrobial resistance of enterococcal isolates from Southern region of Brazil |
title_full |
Genetic diversity and antimicrobial resistance of enterococcal isolates from Southern region of Brazil |
title_fullStr |
Genetic diversity and antimicrobial resistance of enterococcal isolates from Southern region of Brazil |
title_full_unstemmed |
Genetic diversity and antimicrobial resistance of enterococcal isolates from Southern region of Brazil |
title_sort |
genetic diversity and antimicrobial resistance of enterococcal isolates from southern region of brazil |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2006 |
url |
https://doaj.org/article/455dc501a6d5409bbd63ed4536b377f5 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 48, Iss 1, Pp 11-16 (2006) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652006000100003&tlng=en https://doaj.org/toc/1678-9946 1678-9946 https://doaj.org/article/455dc501a6d5409bbd63ed4536b377f5 |
_version_ |
1809895701567504384 |