Dual RNA-seq of parasite and host reveals gene expression dynamics during filarial worm-mosquito interactions.
Parasite biology, by its very nature, cannot be understood without integrating it with that of the host, nor can the host response be adequately explained without considering the activity of the parasite. However, due to experimental limitations, molecular studies of parasite-host systems have been...
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ftdoajarticles:oai:doaj.org/article:455c87f9c8b6413e84b8c4bd19aea4b1 2023-05-15T15:14:46+02:00 Dual RNA-seq of parasite and host reveals gene expression dynamics during filarial worm-mosquito interactions. Young-Jun Choi Matthew T Aliota George F Mayhew Sara M Erickson Bruce M Christensen 2014-05-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0002905 https://doaj.org/article/455c87f9c8b6413e84b8c4bd19aea4b1 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4031193?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002905 https://doaj.org/article/455c87f9c8b6413e84b8c4bd19aea4b1 PLoS Neglected Tropical Diseases, Vol 8, Iss 5, p e2905 (2014) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2014 ftdoajarticles https://doi.org/10.1371/journal.pntd.0002905 2022-12-31T14:13:26Z Parasite biology, by its very nature, cannot be understood without integrating it with that of the host, nor can the host response be adequately explained without considering the activity of the parasite. However, due to experimental limitations, molecular studies of parasite-host systems have been predominantly one-sided investigations focusing on either of the partners involved. Here, we conducted a dual RNA-seq time course analysis of filarial worm parasite and host mosquito to better understand the parasite processes underlying development in and interaction with the host tissue, from the establishment of infection to the development of infective-stage larva.Using the Brugia malayi-Aedes aegypti system, we report parasite gene transcription dynamics, which exhibited a highly ordered developmental program consisting of a series of cyclical and state-transitioning temporal patterns. In addition, we contextualized these parasite data in relation to the concurrent dynamics of the host transcriptome. Comparative analyses using uninfected tissues and different host strains revealed the influence of parasite development on host gene transcription as well as the influence of the host environment on parasite gene transcription. We also critically evaluated the life-cycle transcriptome of B. malayi by comparing developmental stages in the mosquito relative to those in the mammalian host, providing insight into gene expression changes underpinning the mosquito-borne parasitic lifestyle of this heteroxenous parasite.The data presented herein provide the research community with information to design wet lab experiments and select candidates for future study to more fully dissect the whole set of molecular interactions of both organisms in this mosquito-filarial worm symbiotic relationship. Furthermore, characterization of the transcriptional program over the complete life cycle of the parasite, including stages within the mosquito, could help devise novel targets for control strategies. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 8 5 e2905 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Young-Jun Choi Matthew T Aliota George F Mayhew Sara M Erickson Bruce M Christensen Dual RNA-seq of parasite and host reveals gene expression dynamics during filarial worm-mosquito interactions. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Parasite biology, by its very nature, cannot be understood without integrating it with that of the host, nor can the host response be adequately explained without considering the activity of the parasite. However, due to experimental limitations, molecular studies of parasite-host systems have been predominantly one-sided investigations focusing on either of the partners involved. Here, we conducted a dual RNA-seq time course analysis of filarial worm parasite and host mosquito to better understand the parasite processes underlying development in and interaction with the host tissue, from the establishment of infection to the development of infective-stage larva.Using the Brugia malayi-Aedes aegypti system, we report parasite gene transcription dynamics, which exhibited a highly ordered developmental program consisting of a series of cyclical and state-transitioning temporal patterns. In addition, we contextualized these parasite data in relation to the concurrent dynamics of the host transcriptome. Comparative analyses using uninfected tissues and different host strains revealed the influence of parasite development on host gene transcription as well as the influence of the host environment on parasite gene transcription. We also critically evaluated the life-cycle transcriptome of B. malayi by comparing developmental stages in the mosquito relative to those in the mammalian host, providing insight into gene expression changes underpinning the mosquito-borne parasitic lifestyle of this heteroxenous parasite.The data presented herein provide the research community with information to design wet lab experiments and select candidates for future study to more fully dissect the whole set of molecular interactions of both organisms in this mosquito-filarial worm symbiotic relationship. Furthermore, characterization of the transcriptional program over the complete life cycle of the parasite, including stages within the mosquito, could help devise novel targets for control strategies. |
format |
Article in Journal/Newspaper |
author |
Young-Jun Choi Matthew T Aliota George F Mayhew Sara M Erickson Bruce M Christensen |
author_facet |
Young-Jun Choi Matthew T Aliota George F Mayhew Sara M Erickson Bruce M Christensen |
author_sort |
Young-Jun Choi |
title |
Dual RNA-seq of parasite and host reveals gene expression dynamics during filarial worm-mosquito interactions. |
title_short |
Dual RNA-seq of parasite and host reveals gene expression dynamics during filarial worm-mosquito interactions. |
title_full |
Dual RNA-seq of parasite and host reveals gene expression dynamics during filarial worm-mosquito interactions. |
title_fullStr |
Dual RNA-seq of parasite and host reveals gene expression dynamics during filarial worm-mosquito interactions. |
title_full_unstemmed |
Dual RNA-seq of parasite and host reveals gene expression dynamics during filarial worm-mosquito interactions. |
title_sort |
dual rna-seq of parasite and host reveals gene expression dynamics during filarial worm-mosquito interactions. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2014 |
url |
https://doi.org/10.1371/journal.pntd.0002905 https://doaj.org/article/455c87f9c8b6413e84b8c4bd19aea4b1 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 8, Iss 5, p e2905 (2014) |
op_relation |
http://europepmc.org/articles/PMC4031193?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002905 https://doaj.org/article/455c87f9c8b6413e84b8c4bd19aea4b1 |
op_doi |
https://doi.org/10.1371/journal.pntd.0002905 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
8 |
container_issue |
5 |
container_start_page |
e2905 |
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1766345182464180224 |