Comparison of visual and automated Deki Reader interpretation of malaria rapid diagnostic tests in rural Tanzanian military health facilities
Abstract Background Although microscopy is a standard diagnostic tool for malaria and the gold standard, it is infrequently used because of unavailability of laboratory facilities and the absence of skilled readers in poor resource settings. Malaria rapid diagnostic tests (RDT) are currently used in...
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ftdoajarticles:oai:doaj.org/article:4504f1567eaa4f79b40e6f5bc366d585 2023-05-15T15:14:14+02:00 Comparison of visual and automated Deki Reader interpretation of malaria rapid diagnostic tests in rural Tanzanian military health facilities Akili K. Kalinga Charles Mwanziva Sarah Chiduo Christopher Mswanya Deus I. Ishengoma Filbert Francis Lucky Temu Lucas Mahikwano Saidi Mgata George Amoo Lalaine Anova Eyako Wurrapa Nora Zwingerman Santiago Ferro Geeta Bhat Ian Fine Brian Vesely Norman Waters Mara Kreishman-Deitrick Mark Hickman Robert Paris Edwin Kamau Colin Ohrt Reginald A. Kavishe 2018-05-01T00:00:00Z https://doi.org/10.1186/s12936-018-2363-9 https://doaj.org/article/4504f1567eaa4f79b40e6f5bc366d585 EN eng BMC http://link.springer.com/article/10.1186/s12936-018-2363-9 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-018-2363-9 1475-2875 https://doaj.org/article/4504f1567eaa4f79b40e6f5bc366d585 Malaria Journal, Vol 17, Iss 1, Pp 1-10 (2018) Malaria Automated Deki Reader RDT Interpretation Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2018 ftdoajarticles https://doi.org/10.1186/s12936-018-2363-9 2022-12-31T02:16:41Z Abstract Background Although microscopy is a standard diagnostic tool for malaria and the gold standard, it is infrequently used because of unavailability of laboratory facilities and the absence of skilled readers in poor resource settings. Malaria rapid diagnostic tests (RDT) are currently used instead of or as an adjunct to microscopy. However, at very low parasitaemia (usually < 100 asexual parasites/µl), the test line on malaria rapid diagnostic tests can be faint and consequently hard to visualize and this may potentially affect the interpretation of the test results. Fio Corporation (Canada), developed an automated RDT reader named Deki Reader™ for automatic analysis and interpretation of rapid diagnostic tests. This study aimed to compare visual assessment and automated Deki Reader evaluations to interpret malaria rapid diagnostic tests against microscopy. Unlike in the previous studies where expert laboratory technicians interpreted the test results visually and operated the device, in this study low cadre health care workers who have not attended any formal professional training in laboratory sciences were employed. Methods Finger prick blood from 1293 outpatients with fever was tested for malaria using RDT and Giemsa-stained microscopy for thick and thin blood smears. Blood samples for RDTs were processed according to manufacturers’ instructions automated in the Deki Reader. Results of malaria diagnoses were compared between visual and the automated devise reading of RDT and microscopy. Results The sensitivity of malaria rapid diagnostic test results interpreted by the Deki Reader was 94.1% and that of visual interpretation was 93.9%. The specificity of malaria rapid diagnostic test results was 71.8% and that of human interpretation was 72.0%. The positive predictive value of malaria RDT results by the Deki Reader and visual interpretation was 75.8 and 75.4%, respectively, while the negative predictive values were 92.8 and 92.4%, respectively. The accuracy of RDT as interpreted by DR and visually ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Canada Malaria Journal 17 1 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Malaria Automated Deki Reader RDT Interpretation Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Malaria Automated Deki Reader RDT Interpretation Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Akili K. Kalinga Charles Mwanziva Sarah Chiduo Christopher Mswanya Deus I. Ishengoma Filbert Francis Lucky Temu Lucas Mahikwano Saidi Mgata George Amoo Lalaine Anova Eyako Wurrapa Nora Zwingerman Santiago Ferro Geeta Bhat Ian Fine Brian Vesely Norman Waters Mara Kreishman-Deitrick Mark Hickman Robert Paris Edwin Kamau Colin Ohrt Reginald A. Kavishe Comparison of visual and automated Deki Reader interpretation of malaria rapid diagnostic tests in rural Tanzanian military health facilities |
topic_facet |
Malaria Automated Deki Reader RDT Interpretation Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Although microscopy is a standard diagnostic tool for malaria and the gold standard, it is infrequently used because of unavailability of laboratory facilities and the absence of skilled readers in poor resource settings. Malaria rapid diagnostic tests (RDT) are currently used instead of or as an adjunct to microscopy. However, at very low parasitaemia (usually < 100 asexual parasites/µl), the test line on malaria rapid diagnostic tests can be faint and consequently hard to visualize and this may potentially affect the interpretation of the test results. Fio Corporation (Canada), developed an automated RDT reader named Deki Reader™ for automatic analysis and interpretation of rapid diagnostic tests. This study aimed to compare visual assessment and automated Deki Reader evaluations to interpret malaria rapid diagnostic tests against microscopy. Unlike in the previous studies where expert laboratory technicians interpreted the test results visually and operated the device, in this study low cadre health care workers who have not attended any formal professional training in laboratory sciences were employed. Methods Finger prick blood from 1293 outpatients with fever was tested for malaria using RDT and Giemsa-stained microscopy for thick and thin blood smears. Blood samples for RDTs were processed according to manufacturers’ instructions automated in the Deki Reader. Results of malaria diagnoses were compared between visual and the automated devise reading of RDT and microscopy. Results The sensitivity of malaria rapid diagnostic test results interpreted by the Deki Reader was 94.1% and that of visual interpretation was 93.9%. The specificity of malaria rapid diagnostic test results was 71.8% and that of human interpretation was 72.0%. The positive predictive value of malaria RDT results by the Deki Reader and visual interpretation was 75.8 and 75.4%, respectively, while the negative predictive values were 92.8 and 92.4%, respectively. The accuracy of RDT as interpreted by DR and visually ... |
format |
Article in Journal/Newspaper |
author |
Akili K. Kalinga Charles Mwanziva Sarah Chiduo Christopher Mswanya Deus I. Ishengoma Filbert Francis Lucky Temu Lucas Mahikwano Saidi Mgata George Amoo Lalaine Anova Eyako Wurrapa Nora Zwingerman Santiago Ferro Geeta Bhat Ian Fine Brian Vesely Norman Waters Mara Kreishman-Deitrick Mark Hickman Robert Paris Edwin Kamau Colin Ohrt Reginald A. Kavishe |
author_facet |
Akili K. Kalinga Charles Mwanziva Sarah Chiduo Christopher Mswanya Deus I. Ishengoma Filbert Francis Lucky Temu Lucas Mahikwano Saidi Mgata George Amoo Lalaine Anova Eyako Wurrapa Nora Zwingerman Santiago Ferro Geeta Bhat Ian Fine Brian Vesely Norman Waters Mara Kreishman-Deitrick Mark Hickman Robert Paris Edwin Kamau Colin Ohrt Reginald A. Kavishe |
author_sort |
Akili K. Kalinga |
title |
Comparison of visual and automated Deki Reader interpretation of malaria rapid diagnostic tests in rural Tanzanian military health facilities |
title_short |
Comparison of visual and automated Deki Reader interpretation of malaria rapid diagnostic tests in rural Tanzanian military health facilities |
title_full |
Comparison of visual and automated Deki Reader interpretation of malaria rapid diagnostic tests in rural Tanzanian military health facilities |
title_fullStr |
Comparison of visual and automated Deki Reader interpretation of malaria rapid diagnostic tests in rural Tanzanian military health facilities |
title_full_unstemmed |
Comparison of visual and automated Deki Reader interpretation of malaria rapid diagnostic tests in rural Tanzanian military health facilities |
title_sort |
comparison of visual and automated deki reader interpretation of malaria rapid diagnostic tests in rural tanzanian military health facilities |
publisher |
BMC |
publishDate |
2018 |
url |
https://doi.org/10.1186/s12936-018-2363-9 https://doaj.org/article/4504f1567eaa4f79b40e6f5bc366d585 |
geographic |
Arctic Canada |
geographic_facet |
Arctic Canada |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 17, Iss 1, Pp 1-10 (2018) |
op_relation |
http://link.springer.com/article/10.1186/s12936-018-2363-9 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-018-2363-9 1475-2875 https://doaj.org/article/4504f1567eaa4f79b40e6f5bc366d585 |
op_doi |
https://doi.org/10.1186/s12936-018-2363-9 |
container_title |
Malaria Journal |
container_volume |
17 |
container_issue |
1 |
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1766344712106541056 |