Comparison of visual and automated Deki Reader interpretation of malaria rapid diagnostic tests in rural Tanzanian military health facilities

Abstract Background Although microscopy is a standard diagnostic tool for malaria and the gold standard, it is infrequently used because of unavailability of laboratory facilities and the absence of skilled readers in poor resource settings. Malaria rapid diagnostic tests (RDT) are currently used in...

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Published in:Malaria Journal
Main Authors: Akili K. Kalinga, Charles Mwanziva, Sarah Chiduo, Christopher Mswanya, Deus I. Ishengoma, Filbert Francis, Lucky Temu, Lucas Mahikwano, Saidi Mgata, George Amoo, Lalaine Anova, Eyako Wurrapa, Nora Zwingerman, Santiago Ferro, Geeta Bhat, Ian Fine, Brian Vesely, Norman Waters, Mara Kreishman-Deitrick, Mark Hickman, Robert Paris, Edwin Kamau, Colin Ohrt, Reginald A. Kavishe
Format: Article in Journal/Newspaper
Language:English
Published: BMC 2018
Subjects:
RDT
Online Access:https://doi.org/10.1186/s12936-018-2363-9
https://doaj.org/article/4504f1567eaa4f79b40e6f5bc366d585
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spelling ftdoajarticles:oai:doaj.org/article:4504f1567eaa4f79b40e6f5bc366d585 2023-05-15T15:14:14+02:00 Comparison of visual and automated Deki Reader interpretation of malaria rapid diagnostic tests in rural Tanzanian military health facilities Akili K. Kalinga Charles Mwanziva Sarah Chiduo Christopher Mswanya Deus I. Ishengoma Filbert Francis Lucky Temu Lucas Mahikwano Saidi Mgata George Amoo Lalaine Anova Eyako Wurrapa Nora Zwingerman Santiago Ferro Geeta Bhat Ian Fine Brian Vesely Norman Waters Mara Kreishman-Deitrick Mark Hickman Robert Paris Edwin Kamau Colin Ohrt Reginald A. Kavishe 2018-05-01T00:00:00Z https://doi.org/10.1186/s12936-018-2363-9 https://doaj.org/article/4504f1567eaa4f79b40e6f5bc366d585 EN eng BMC http://link.springer.com/article/10.1186/s12936-018-2363-9 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-018-2363-9 1475-2875 https://doaj.org/article/4504f1567eaa4f79b40e6f5bc366d585 Malaria Journal, Vol 17, Iss 1, Pp 1-10 (2018) Malaria Automated Deki Reader RDT Interpretation Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2018 ftdoajarticles https://doi.org/10.1186/s12936-018-2363-9 2022-12-31T02:16:41Z Abstract Background Although microscopy is a standard diagnostic tool for malaria and the gold standard, it is infrequently used because of unavailability of laboratory facilities and the absence of skilled readers in poor resource settings. Malaria rapid diagnostic tests (RDT) are currently used instead of or as an adjunct to microscopy. However, at very low parasitaemia (usually < 100 asexual parasites/µl), the test line on malaria rapid diagnostic tests can be faint and consequently hard to visualize and this may potentially affect the interpretation of the test results. Fio Corporation (Canada), developed an automated RDT reader named Deki Reader™ for automatic analysis and interpretation of rapid diagnostic tests. This study aimed to compare visual assessment and automated Deki Reader evaluations to interpret malaria rapid diagnostic tests against microscopy. Unlike in the previous studies where expert laboratory technicians interpreted the test results visually and operated the device, in this study low cadre health care workers who have not attended any formal professional training in laboratory sciences were employed. Methods Finger prick blood from 1293 outpatients with fever was tested for malaria using RDT and Giemsa-stained microscopy for thick and thin blood smears. Blood samples for RDTs were processed according to manufacturers’ instructions automated in the Deki Reader. Results of malaria diagnoses were compared between visual and the automated devise reading of RDT and microscopy. Results The sensitivity of malaria rapid diagnostic test results interpreted by the Deki Reader was 94.1% and that of visual interpretation was 93.9%. The specificity of malaria rapid diagnostic test results was 71.8% and that of human interpretation was 72.0%. The positive predictive value of malaria RDT results by the Deki Reader and visual interpretation was 75.8 and 75.4%, respectively, while the negative predictive values were 92.8 and 92.4%, respectively. The accuracy of RDT as interpreted by DR and visually ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Canada Malaria Journal 17 1
institution Open Polar
collection Directory of Open Access Journals: DOAJ Articles
op_collection_id ftdoajarticles
language English
topic Malaria
Automated
Deki Reader
RDT
Interpretation
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
spellingShingle Malaria
Automated
Deki Reader
RDT
Interpretation
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
Akili K. Kalinga
Charles Mwanziva
Sarah Chiduo
Christopher Mswanya
Deus I. Ishengoma
Filbert Francis
Lucky Temu
Lucas Mahikwano
Saidi Mgata
George Amoo
Lalaine Anova
Eyako Wurrapa
Nora Zwingerman
Santiago Ferro
Geeta Bhat
Ian Fine
Brian Vesely
Norman Waters
Mara Kreishman-Deitrick
Mark Hickman
Robert Paris
Edwin Kamau
Colin Ohrt
Reginald A. Kavishe
Comparison of visual and automated Deki Reader interpretation of malaria rapid diagnostic tests in rural Tanzanian military health facilities
topic_facet Malaria
Automated
Deki Reader
RDT
Interpretation
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
description Abstract Background Although microscopy is a standard diagnostic tool for malaria and the gold standard, it is infrequently used because of unavailability of laboratory facilities and the absence of skilled readers in poor resource settings. Malaria rapid diagnostic tests (RDT) are currently used instead of or as an adjunct to microscopy. However, at very low parasitaemia (usually < 100 asexual parasites/µl), the test line on malaria rapid diagnostic tests can be faint and consequently hard to visualize and this may potentially affect the interpretation of the test results. Fio Corporation (Canada), developed an automated RDT reader named Deki Reader™ for automatic analysis and interpretation of rapid diagnostic tests. This study aimed to compare visual assessment and automated Deki Reader evaluations to interpret malaria rapid diagnostic tests against microscopy. Unlike in the previous studies where expert laboratory technicians interpreted the test results visually and operated the device, in this study low cadre health care workers who have not attended any formal professional training in laboratory sciences were employed. Methods Finger prick blood from 1293 outpatients with fever was tested for malaria using RDT and Giemsa-stained microscopy for thick and thin blood smears. Blood samples for RDTs were processed according to manufacturers’ instructions automated in the Deki Reader. Results of malaria diagnoses were compared between visual and the automated devise reading of RDT and microscopy. Results The sensitivity of malaria rapid diagnostic test results interpreted by the Deki Reader was 94.1% and that of visual interpretation was 93.9%. The specificity of malaria rapid diagnostic test results was 71.8% and that of human interpretation was 72.0%. The positive predictive value of malaria RDT results by the Deki Reader and visual interpretation was 75.8 and 75.4%, respectively, while the negative predictive values were 92.8 and 92.4%, respectively. The accuracy of RDT as interpreted by DR and visually ...
format Article in Journal/Newspaper
author Akili K. Kalinga
Charles Mwanziva
Sarah Chiduo
Christopher Mswanya
Deus I. Ishengoma
Filbert Francis
Lucky Temu
Lucas Mahikwano
Saidi Mgata
George Amoo
Lalaine Anova
Eyako Wurrapa
Nora Zwingerman
Santiago Ferro
Geeta Bhat
Ian Fine
Brian Vesely
Norman Waters
Mara Kreishman-Deitrick
Mark Hickman
Robert Paris
Edwin Kamau
Colin Ohrt
Reginald A. Kavishe
author_facet Akili K. Kalinga
Charles Mwanziva
Sarah Chiduo
Christopher Mswanya
Deus I. Ishengoma
Filbert Francis
Lucky Temu
Lucas Mahikwano
Saidi Mgata
George Amoo
Lalaine Anova
Eyako Wurrapa
Nora Zwingerman
Santiago Ferro
Geeta Bhat
Ian Fine
Brian Vesely
Norman Waters
Mara Kreishman-Deitrick
Mark Hickman
Robert Paris
Edwin Kamau
Colin Ohrt
Reginald A. Kavishe
author_sort Akili K. Kalinga
title Comparison of visual and automated Deki Reader interpretation of malaria rapid diagnostic tests in rural Tanzanian military health facilities
title_short Comparison of visual and automated Deki Reader interpretation of malaria rapid diagnostic tests in rural Tanzanian military health facilities
title_full Comparison of visual and automated Deki Reader interpretation of malaria rapid diagnostic tests in rural Tanzanian military health facilities
title_fullStr Comparison of visual and automated Deki Reader interpretation of malaria rapid diagnostic tests in rural Tanzanian military health facilities
title_full_unstemmed Comparison of visual and automated Deki Reader interpretation of malaria rapid diagnostic tests in rural Tanzanian military health facilities
title_sort comparison of visual and automated deki reader interpretation of malaria rapid diagnostic tests in rural tanzanian military health facilities
publisher BMC
publishDate 2018
url https://doi.org/10.1186/s12936-018-2363-9
https://doaj.org/article/4504f1567eaa4f79b40e6f5bc366d585
geographic Arctic
Canada
geographic_facet Arctic
Canada
genre Arctic
genre_facet Arctic
op_source Malaria Journal, Vol 17, Iss 1, Pp 1-10 (2018)
op_relation http://link.springer.com/article/10.1186/s12936-018-2363-9
https://doaj.org/toc/1475-2875
doi:10.1186/s12936-018-2363-9
1475-2875
https://doaj.org/article/4504f1567eaa4f79b40e6f5bc366d585
op_doi https://doi.org/10.1186/s12936-018-2363-9
container_title Malaria Journal
container_volume 17
container_issue 1
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