Quantification of dengue virus specific T cell responses and correlation with viral load and clinical disease severity in acute dengue infection.
Background In order to understand the role of dengue virus (DENV) specific T cell responses that associate with protection, we studied their frequency and phenotype in relation to clinical disease severity and resolution of viraemia in a large cohort of patients with varying severity of acute dengue...
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ftdoajarticles:oai:doaj.org/article:4500cf6fd9a143fda51366419196ad32 2023-05-15T15:13:27+02:00 Quantification of dengue virus specific T cell responses and correlation with viral load and clinical disease severity in acute dengue infection. Dulharie T Wijeratne Samitha Fernando Laksiri Gomes Chandima Jeewandara Anushka Ginneliya Supun Samarasekara Ananda Wijewickrama Clare S Hardman Graham S Ogg Gathsaurie Neelika Malavige 2018-10-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0006540 https://doaj.org/article/4500cf6fd9a143fda51366419196ad32 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0006540 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006540 https://doaj.org/article/4500cf6fd9a143fda51366419196ad32 PLoS Neglected Tropical Diseases, Vol 12, Iss 10, p e0006540 (2018) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2018 ftdoajarticles https://doi.org/10.1371/journal.pntd.0006540 2022-12-31T07:48:36Z Background In order to understand the role of dengue virus (DENV) specific T cell responses that associate with protection, we studied their frequency and phenotype in relation to clinical disease severity and resolution of viraemia in a large cohort of patients with varying severity of acute dengue infection. Methodology/principal findings Using ex vivo IFNγ ELISpot assays we determined the frequency of dengue viral peptide (DENV)-NS3, NS1 and NS5 responsive T cells in 74 adult patients with acute dengue infection and examined the association of responsive T cell frequency with the extent of viraemia and clinical disease severity. We found that total DENV-specific and DENV-NS3-specific T cell responses, were higher in patients with dengue fever (DF), when compared to those with dengue haemorrhagic fever (DHF). In addition, those with DF had significantly higher (p = 0.02) DENV-specific T cell responses on day 4 of infection compared to those who subsequently developed DHF. DENV peptide specific T cell responses inversely correlated with the degree of viraemia, which was most significant for DENV-NS3 specific T cell responses (Spearman's r = -0.47, p = 0.0003). The frequency of T cell responses to NS1, NS5 and pooled DENV peptides, correlated with the degree of thrombocytopenia but had no association with levels of liver transaminases. In contrast, total DENV-IgG inversely correlated with the degree of thrombocytopenia and levels of liver transaminases. Conclusions/significance Early appearance of DENV-specific T cell IFNγ responses before the onset of plasma leakage, appears to associate with milder clinical disease and resolution of viraemia, suggesting a protective role in acute dengue infection. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 12 10 e0006540 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Dulharie T Wijeratne Samitha Fernando Laksiri Gomes Chandima Jeewandara Anushka Ginneliya Supun Samarasekara Ananda Wijewickrama Clare S Hardman Graham S Ogg Gathsaurie Neelika Malavige Quantification of dengue virus specific T cell responses and correlation with viral load and clinical disease severity in acute dengue infection. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background In order to understand the role of dengue virus (DENV) specific T cell responses that associate with protection, we studied their frequency and phenotype in relation to clinical disease severity and resolution of viraemia in a large cohort of patients with varying severity of acute dengue infection. Methodology/principal findings Using ex vivo IFNγ ELISpot assays we determined the frequency of dengue viral peptide (DENV)-NS3, NS1 and NS5 responsive T cells in 74 adult patients with acute dengue infection and examined the association of responsive T cell frequency with the extent of viraemia and clinical disease severity. We found that total DENV-specific and DENV-NS3-specific T cell responses, were higher in patients with dengue fever (DF), when compared to those with dengue haemorrhagic fever (DHF). In addition, those with DF had significantly higher (p = 0.02) DENV-specific T cell responses on day 4 of infection compared to those who subsequently developed DHF. DENV peptide specific T cell responses inversely correlated with the degree of viraemia, which was most significant for DENV-NS3 specific T cell responses (Spearman's r = -0.47, p = 0.0003). The frequency of T cell responses to NS1, NS5 and pooled DENV peptides, correlated with the degree of thrombocytopenia but had no association with levels of liver transaminases. In contrast, total DENV-IgG inversely correlated with the degree of thrombocytopenia and levels of liver transaminases. Conclusions/significance Early appearance of DENV-specific T cell IFNγ responses before the onset of plasma leakage, appears to associate with milder clinical disease and resolution of viraemia, suggesting a protective role in acute dengue infection. |
format |
Article in Journal/Newspaper |
author |
Dulharie T Wijeratne Samitha Fernando Laksiri Gomes Chandima Jeewandara Anushka Ginneliya Supun Samarasekara Ananda Wijewickrama Clare S Hardman Graham S Ogg Gathsaurie Neelika Malavige |
author_facet |
Dulharie T Wijeratne Samitha Fernando Laksiri Gomes Chandima Jeewandara Anushka Ginneliya Supun Samarasekara Ananda Wijewickrama Clare S Hardman Graham S Ogg Gathsaurie Neelika Malavige |
author_sort |
Dulharie T Wijeratne |
title |
Quantification of dengue virus specific T cell responses and correlation with viral load and clinical disease severity in acute dengue infection. |
title_short |
Quantification of dengue virus specific T cell responses and correlation with viral load and clinical disease severity in acute dengue infection. |
title_full |
Quantification of dengue virus specific T cell responses and correlation with viral load and clinical disease severity in acute dengue infection. |
title_fullStr |
Quantification of dengue virus specific T cell responses and correlation with viral load and clinical disease severity in acute dengue infection. |
title_full_unstemmed |
Quantification of dengue virus specific T cell responses and correlation with viral load and clinical disease severity in acute dengue infection. |
title_sort |
quantification of dengue virus specific t cell responses and correlation with viral load and clinical disease severity in acute dengue infection. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2018 |
url |
https://doi.org/10.1371/journal.pntd.0006540 https://doaj.org/article/4500cf6fd9a143fda51366419196ad32 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 12, Iss 10, p e0006540 (2018) |
op_relation |
https://doi.org/10.1371/journal.pntd.0006540 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006540 https://doaj.org/article/4500cf6fd9a143fda51366419196ad32 |
op_doi |
https://doi.org/10.1371/journal.pntd.0006540 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
12 |
container_issue |
10 |
container_start_page |
e0006540 |
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1766343997897310208 |