Nanolitre real-time PCR detection of bacterial, parasitic, and viral agents from patients with diarrhoea in Nunavut, Canada
Background. Little is known about the microbiology of diarrhoeal disease in Canada's Arctic regions. There are a number of limitations of conventional microbiology testing techniques for diarrhoeal pathogens, and these may be further compromised in the Arctic, given the often long distanc...
Published in: | International Journal of Circumpolar Health |
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Taylor & Francis Group
2013
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Online Access: | https://doi.org/10.3402/ijch.v72i0.19903 https://doaj.org/article/44faf589c64148559c3ea74fdb910470 |
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ftdoajarticles:oai:doaj.org/article:44faf589c64148559c3ea74fdb910470 2023-05-15T14:53:07+02:00 Nanolitre real-time PCR detection of bacterial, parasitic, and viral agents from patients with diarrhoea in Nunavut, Canada David M. Goldfarb Brent Dixon Ioana Moldovan Nicholas Barrowman Kirsten Mattison Chad Zentner Maureen Baikie Sabah Bidawid Francis Chan Robert Slinger 2013-04-01T00:00:00Z https://doi.org/10.3402/ijch.v72i0.19903 https://doaj.org/article/44faf589c64148559c3ea74fdb910470 EN eng Taylor & Francis Group http://www.circumpolarhealthjournal.net/index.php/ijch/article/download/19903/pdf_1 https://doaj.org/toc/2242-3982 doi:10.3402/ijch.v72i0.19903 2242-3982 https://doaj.org/article/44faf589c64148559c3ea74fdb910470 International Journal of Circumpolar Health, Vol 72, Iss 0, Pp 1-8 (2013) multiplex real-time PCR detection pathogens Campylobacter spp Cryptosporidium spp Clostridium difficile viral agents diarrhoea gastroenteritis Nunavut Arctic Arctic medicine. Tropical medicine RC955-962 article 2013 ftdoajarticles https://doi.org/10.3402/ijch.v72i0.19903 2022-12-31T15:08:31Z Background. Little is known about the microbiology of diarrhoeal disease in Canada's Arctic regions. There are a number of limitations of conventional microbiology testing techniques for diarrhoeal pathogens, and these may be further compromised in the Arctic, given the often long distances for specimen transport. Objective. To develop a novel multiple-target nanolitre real-time reverse transcriptase (RT)-PCR platform to simultaneously test diarrhoeal specimens collected from residents of the Qikiqtani (Baffin Island) Region of Nunavut, Canada, for a wide range of bacterial, parasitic and viral agents. Study design/methods. Diarrhoeal stool samples submitted for bacterial culture to Qikiqtani General Hospital in Nunavut over an 18-month period were tested with a multiple-target nanolitre real-time PCR panel for major diarrhoeal pathogens including 8 bacterial, 6 viral and 2 parasitic targets. Results. Among 86 stool specimens tested by PCR, a total of 50 pathogens were detected with 1 or more pathogens found in 40 (46.5%) stool specimens. The organisms detected comprised 17 Cryptosporidium spp., 5 Clostridium difficile with toxin B, 6 Campylobacter spp., 6 Salmonella spp., 4 astroviruses, 3 noroviruses, 1 rotavirus, 1 Shigella spp. and 1 Giardia spp. The frequency of detection by PCR and bacterial culture was similar for Salmonella spp., but discrepant for Campylobacter spp., as Campylobacter was detected by culture from only 1/86 specimens. Similarly, Cryptosporidium spp. was detected in multiple samples by PCR but was not detected by microscopy or enzyme immunoassay. Conclusions. Cryptosporidium spp., Campylobacter spp. and Clostridium difficile may be relatively common but possibly under-recognised pathogens in this region. Further study is needed to determine the regional epidemiology and clinical significance of these organisms. This method appears to be a useful tool for gastrointestinal pathogen research and may also be helpful for clinical diagnostics and outbreak investigation in remote ... Article in Journal/Newspaper Arctic Baffin Island Baffin Circumpolar Health International Journal of Circumpolar Health Nunavut Directory of Open Access Journals: DOAJ Articles Arctic Nunavut Baffin Island Canada International Journal of Circumpolar Health 72 1 19903 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
multiplex real-time PCR detection pathogens Campylobacter spp Cryptosporidium spp Clostridium difficile viral agents diarrhoea gastroenteritis Nunavut Arctic Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
multiplex real-time PCR detection pathogens Campylobacter spp Cryptosporidium spp Clostridium difficile viral agents diarrhoea gastroenteritis Nunavut Arctic Arctic medicine. Tropical medicine RC955-962 David M. Goldfarb Brent Dixon Ioana Moldovan Nicholas Barrowman Kirsten Mattison Chad Zentner Maureen Baikie Sabah Bidawid Francis Chan Robert Slinger Nanolitre real-time PCR detection of bacterial, parasitic, and viral agents from patients with diarrhoea in Nunavut, Canada |
topic_facet |
multiplex real-time PCR detection pathogens Campylobacter spp Cryptosporidium spp Clostridium difficile viral agents diarrhoea gastroenteritis Nunavut Arctic Arctic medicine. Tropical medicine RC955-962 |
description |
Background. Little is known about the microbiology of diarrhoeal disease in Canada's Arctic regions. There are a number of limitations of conventional microbiology testing techniques for diarrhoeal pathogens, and these may be further compromised in the Arctic, given the often long distances for specimen transport. Objective. To develop a novel multiple-target nanolitre real-time reverse transcriptase (RT)-PCR platform to simultaneously test diarrhoeal specimens collected from residents of the Qikiqtani (Baffin Island) Region of Nunavut, Canada, for a wide range of bacterial, parasitic and viral agents. Study design/methods. Diarrhoeal stool samples submitted for bacterial culture to Qikiqtani General Hospital in Nunavut over an 18-month period were tested with a multiple-target nanolitre real-time PCR panel for major diarrhoeal pathogens including 8 bacterial, 6 viral and 2 parasitic targets. Results. Among 86 stool specimens tested by PCR, a total of 50 pathogens were detected with 1 or more pathogens found in 40 (46.5%) stool specimens. The organisms detected comprised 17 Cryptosporidium spp., 5 Clostridium difficile with toxin B, 6 Campylobacter spp., 6 Salmonella spp., 4 astroviruses, 3 noroviruses, 1 rotavirus, 1 Shigella spp. and 1 Giardia spp. The frequency of detection by PCR and bacterial culture was similar for Salmonella spp., but discrepant for Campylobacter spp., as Campylobacter was detected by culture from only 1/86 specimens. Similarly, Cryptosporidium spp. was detected in multiple samples by PCR but was not detected by microscopy or enzyme immunoassay. Conclusions. Cryptosporidium spp., Campylobacter spp. and Clostridium difficile may be relatively common but possibly under-recognised pathogens in this region. Further study is needed to determine the regional epidemiology and clinical significance of these organisms. This method appears to be a useful tool for gastrointestinal pathogen research and may also be helpful for clinical diagnostics and outbreak investigation in remote ... |
format |
Article in Journal/Newspaper |
author |
David M. Goldfarb Brent Dixon Ioana Moldovan Nicholas Barrowman Kirsten Mattison Chad Zentner Maureen Baikie Sabah Bidawid Francis Chan Robert Slinger |
author_facet |
David M. Goldfarb Brent Dixon Ioana Moldovan Nicholas Barrowman Kirsten Mattison Chad Zentner Maureen Baikie Sabah Bidawid Francis Chan Robert Slinger |
author_sort |
David M. Goldfarb |
title |
Nanolitre real-time PCR detection of bacterial, parasitic, and viral agents from patients with diarrhoea in Nunavut, Canada |
title_short |
Nanolitre real-time PCR detection of bacterial, parasitic, and viral agents from patients with diarrhoea in Nunavut, Canada |
title_full |
Nanolitre real-time PCR detection of bacterial, parasitic, and viral agents from patients with diarrhoea in Nunavut, Canada |
title_fullStr |
Nanolitre real-time PCR detection of bacterial, parasitic, and viral agents from patients with diarrhoea in Nunavut, Canada |
title_full_unstemmed |
Nanolitre real-time PCR detection of bacterial, parasitic, and viral agents from patients with diarrhoea in Nunavut, Canada |
title_sort |
nanolitre real-time pcr detection of bacterial, parasitic, and viral agents from patients with diarrhoea in nunavut, canada |
publisher |
Taylor & Francis Group |
publishDate |
2013 |
url |
https://doi.org/10.3402/ijch.v72i0.19903 https://doaj.org/article/44faf589c64148559c3ea74fdb910470 |
geographic |
Arctic Nunavut Baffin Island Canada |
geographic_facet |
Arctic Nunavut Baffin Island Canada |
genre |
Arctic Baffin Island Baffin Circumpolar Health International Journal of Circumpolar Health Nunavut |
genre_facet |
Arctic Baffin Island Baffin Circumpolar Health International Journal of Circumpolar Health Nunavut |
op_source |
International Journal of Circumpolar Health, Vol 72, Iss 0, Pp 1-8 (2013) |
op_relation |
http://www.circumpolarhealthjournal.net/index.php/ijch/article/download/19903/pdf_1 https://doaj.org/toc/2242-3982 doi:10.3402/ijch.v72i0.19903 2242-3982 https://doaj.org/article/44faf589c64148559c3ea74fdb910470 |
op_doi |
https://doi.org/10.3402/ijch.v72i0.19903 |
container_title |
International Journal of Circumpolar Health |
container_volume |
72 |
container_issue |
1 |
container_start_page |
19903 |
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1766324537191825408 |