Proteomic and deep sequencing analysis of extracellular vesicles isolated from adult male and female Schistosoma japonicum.
Schistosomes are the causative agent of schistosomiasis, which affects more than 200 million people worldwide. Unlike other trematode parasites, schistosomes (along with the Didymozoidae) have evolved separate sexes. Pairing of males and females is a prerequisite for female sexual development and su...
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ftdoajarticles:oai:doaj.org/article:44f7ddbde2424f189b1efcffd945b58e 2023-05-15T15:06:03+02:00 Proteomic and deep sequencing analysis of extracellular vesicles isolated from adult male and female Schistosoma japonicum. Pengfei Du Bikash R Giri Juntao Liu Tianqi Xia Christoph G Grevelding Guofeng Cheng 2020-09-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008618 https://doaj.org/article/44f7ddbde2424f189b1efcffd945b58e EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008618 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008618 https://doaj.org/article/44f7ddbde2424f189b1efcffd945b58e PLoS Neglected Tropical Diseases, Vol 14, Iss 9, p e0008618 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008618 2022-12-31T11:56:29Z Schistosomes are the causative agent of schistosomiasis, which affects more than 200 million people worldwide. Unlike other trematode parasites, schistosomes (along with the Didymozoidae) have evolved separate sexes. Pairing of males and females is a prerequisite for female sexual development and subsequent egg production. However, the mechanisms underlying these processes remain poorly understood. Extracellular vesicles (EVs) have been shown to play important roles in many biological processes. In the present study, we characterized EVs isolated from adult male and female Schistosoma japonicum. Proteomic analyses of the isolated EVs revealed that some proteins are significantly enriched in male or female EVs. RNA-sequencing analysis of a small RNA population associated with EVs identified 18 miRNAs enriched in male and female S. japonicum EVs. Among these, miR-750 was specifically enriched in female EVs. Additionally, the inhibition of miR-750 by a miRNA inhibitor led to decreased egg production in female schistosomes cultured in vitro. Collectively, our results suggest that miR-750 within female EV cargo may be involved in regulating ovary development and egg production in S. japonicum females. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 14 9 e0008618 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Pengfei Du Bikash R Giri Juntao Liu Tianqi Xia Christoph G Grevelding Guofeng Cheng Proteomic and deep sequencing analysis of extracellular vesicles isolated from adult male and female Schistosoma japonicum. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Schistosomes are the causative agent of schistosomiasis, which affects more than 200 million people worldwide. Unlike other trematode parasites, schistosomes (along with the Didymozoidae) have evolved separate sexes. Pairing of males and females is a prerequisite for female sexual development and subsequent egg production. However, the mechanisms underlying these processes remain poorly understood. Extracellular vesicles (EVs) have been shown to play important roles in many biological processes. In the present study, we characterized EVs isolated from adult male and female Schistosoma japonicum. Proteomic analyses of the isolated EVs revealed that some proteins are significantly enriched in male or female EVs. RNA-sequencing analysis of a small RNA population associated with EVs identified 18 miRNAs enriched in male and female S. japonicum EVs. Among these, miR-750 was specifically enriched in female EVs. Additionally, the inhibition of miR-750 by a miRNA inhibitor led to decreased egg production in female schistosomes cultured in vitro. Collectively, our results suggest that miR-750 within female EV cargo may be involved in regulating ovary development and egg production in S. japonicum females. |
format |
Article in Journal/Newspaper |
author |
Pengfei Du Bikash R Giri Juntao Liu Tianqi Xia Christoph G Grevelding Guofeng Cheng |
author_facet |
Pengfei Du Bikash R Giri Juntao Liu Tianqi Xia Christoph G Grevelding Guofeng Cheng |
author_sort |
Pengfei Du |
title |
Proteomic and deep sequencing analysis of extracellular vesicles isolated from adult male and female Schistosoma japonicum. |
title_short |
Proteomic and deep sequencing analysis of extracellular vesicles isolated from adult male and female Schistosoma japonicum. |
title_full |
Proteomic and deep sequencing analysis of extracellular vesicles isolated from adult male and female Schistosoma japonicum. |
title_fullStr |
Proteomic and deep sequencing analysis of extracellular vesicles isolated from adult male and female Schistosoma japonicum. |
title_full_unstemmed |
Proteomic and deep sequencing analysis of extracellular vesicles isolated from adult male and female Schistosoma japonicum. |
title_sort |
proteomic and deep sequencing analysis of extracellular vesicles isolated from adult male and female schistosoma japonicum. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0008618 https://doaj.org/article/44f7ddbde2424f189b1efcffd945b58e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 9, p e0008618 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008618 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008618 https://doaj.org/article/44f7ddbde2424f189b1efcffd945b58e |
op_doi |
https://doi.org/10.1371/journal.pntd.0008618 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
14 |
container_issue |
9 |
container_start_page |
e0008618 |
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1766337718489448448 |