Down-regulated expression of NPM1 in IMS-M2 cell line by (−)-epigallocatechin-3-gallate

• Published in: Asian Pacific Journal of Tropical Biomedicine
• Main Authors: Hoang Thanh Chi, Bui Thi Kim Ly, Hoang Anh Vu, Yuko Sato, Phu Chi Dung, Phan Thi Xinh
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wolters Kluwer Medknow Publications 2014
• Subjects: NPM1; EGCG; IMS-M2; Apoptosis; Arctic medicine. Tropical medicine; RC955-962; Biology (General); QH301-705.5; Arctic
• Online Access: https://doi.org/10.12980/APJTB.4.2014APJTB-2014-0177; https://doaj.org/article/44b3460fa70f4c5886aebf97d6dfd092

Objective: To investigate the inhibited effect of epigallocatechin-3-gallate (EGCG) on the expression of NPM1 in IMS-M2 cells harboring the NPM1 mutations. Methods: Cell proliferation assay was performed to test the effects of EGCG on cell growth of IMS-M2 cells harboring the NPM1 mutations. Western blot analysis were performed to test the protein expression of NPM1, AKT, those associated with apoptosis. Results: EGCG can down-regulate the expression of NPM1 in IMS-M2 cells harboring the NPM1 mutations. Moreover, EGCG also suppressed the cell proliferation and induced apoptosis in IMS-M2 cells. Conclusions: The results suggested that EGCG could be considered as a reagent for treatment of AML patients with NPM1 mutations.