Characterisation of the salmon cystic fibrosis transmembrane conductance regulator protein for structural studies

• Published in: AIMS Molecular Science
• Main Authors: Naomi L. Pollock, Oscar Moran, Debora Baroni, Olga Zegarra-Moran, Robert C. Ford
• Format: Article in Journal/Newspaper
• Language: English
• Published: AIMS Press 2014
• Subjects: CFTR; CFTR purification; salmon; osmoregulation; Saccharomyces cerevisiae; heterologous overexpression; membrane protein purification; cryo-electron microscopy; small-angle X-ray scattering (SAXS); Biology (General); QH301-705.5; Salmo salar
• Online Access: https://doi.org/10.3934/molsci.2014.4.141; https://doaj.org/article/44ae11f3c8c040fdae28099ca76a2f94

The cystic fibrosis transmembrane conductance regulator protein (CFTR) is a chloride channel highly expressed in the gills of Salmo salar, with a role in osmoregulation. It shares 60% identity with the human CFTR channel, mutations to which can cause the common genetic disorder cystic fibrosis CF. The expression and localisation of salmon CFTR have been investigated, but the isolated protein has not been extensively characterised. Here we present a protocol for the purification of recombinant salmon CFTR, along with biophysical and structural characterisation of the purified protein. Salmon CFTR was overexpressed in Saccharomyces cerevisiae, solubilised in the detergent LPG-14 and chromatographically purified by nickel-affinity and size-exclusion chromatography methods. Prior to size-exclusion chromatography samples of salmon CFTR had low purity, and contained large quantities of aggregated protein. Compared to size-exclusion chromatography profiles of other orthologues of CFTR, which had less evidence of aggregation, salmon CFTR appeared to have lower intrinsic stability than human and platypus CFTR. Nonetheless, repeated size-exclusion chromatography allowed monodisperse salmon CFTR to be isolated, and multi-angle light scattering was used to determine its oligomeric state. The monodispersity of the sample and its oligomeric state were confirmed using cryo-electron microscopy and small-angle X-ray scattering (SAXS). These data were also processed to calculate a low-resolution structure of the salmon CFTR, which showed similar architecture to other ATP-binding cassette proteins.