Proteomic analysis of the processes leading to Madurella mycetomatis grain formation in Galleria mellonella larvae.
Mycetoma is a neglected chronic and granulomatous infection primarily associated with the fungal pathogen Madurella mycetomatis. Characteristic of this infection is the formation of grains. However, the processes leading to grain formation are not known. In this study, we employed a proteomic approa...
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ftdoajarticles:oai:doaj.org/article:449e255320ce4cda84c8ce82bbc665c1 2023-05-15T15:07:24+02:00 Proteomic analysis of the processes leading to Madurella mycetomatis grain formation in Galleria mellonella larvae. Gerard Sheehan Mickey Konings Wilson Lim Ahmed Fahal Kevin Kavanagh Wendy W J van de Sande 2020-04-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008190 https://doaj.org/article/449e255320ce4cda84c8ce82bbc665c1 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008190 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008190 https://doaj.org/article/449e255320ce4cda84c8ce82bbc665c1 PLoS Neglected Tropical Diseases, Vol 14, Iss 4, p e0008190 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008190 2022-12-31T05:52:34Z Mycetoma is a neglected chronic and granulomatous infection primarily associated with the fungal pathogen Madurella mycetomatis. Characteristic of this infection is the formation of grains. However, the processes leading to grain formation are not known. In this study, we employed a proteomic approach to characterise M. mycetomatis grain formation in Galleria mellonella larvae and map the processes leading to grain formation over time. For this, at 1 day, 3 days and 7 days post-inoculation, proteins from grains and hemolymph were extracted and analysed by label-free mass spectrometry. A total of 87, 51 and 48 M. mycetomatis proteins and 713, 997, 18 G. mellonella proteins were found in grains on day 1, 3 and 7 post-inoculation respectively. M. mycetomatis proteins were mainly involved in cellular metabolic processes and numerous enzymes were encountered. G. mellonella proteins were primarily involved in the nodulation process. The proteins identified were linked to nodulation and grain formation and four steps of grain formation were identified. The results of this proteomic approach could in the future be used to design novel strategies to interfere with mycetoma grain formation and to combat this difficult to treat infection. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 14 4 e0008190 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Gerard Sheehan Mickey Konings Wilson Lim Ahmed Fahal Kevin Kavanagh Wendy W J van de Sande Proteomic analysis of the processes leading to Madurella mycetomatis grain formation in Galleria mellonella larvae. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Mycetoma is a neglected chronic and granulomatous infection primarily associated with the fungal pathogen Madurella mycetomatis. Characteristic of this infection is the formation of grains. However, the processes leading to grain formation are not known. In this study, we employed a proteomic approach to characterise M. mycetomatis grain formation in Galleria mellonella larvae and map the processes leading to grain formation over time. For this, at 1 day, 3 days and 7 days post-inoculation, proteins from grains and hemolymph were extracted and analysed by label-free mass spectrometry. A total of 87, 51 and 48 M. mycetomatis proteins and 713, 997, 18 G. mellonella proteins were found in grains on day 1, 3 and 7 post-inoculation respectively. M. mycetomatis proteins were mainly involved in cellular metabolic processes and numerous enzymes were encountered. G. mellonella proteins were primarily involved in the nodulation process. The proteins identified were linked to nodulation and grain formation and four steps of grain formation were identified. The results of this proteomic approach could in the future be used to design novel strategies to interfere with mycetoma grain formation and to combat this difficult to treat infection. |
format |
Article in Journal/Newspaper |
author |
Gerard Sheehan Mickey Konings Wilson Lim Ahmed Fahal Kevin Kavanagh Wendy W J van de Sande |
author_facet |
Gerard Sheehan Mickey Konings Wilson Lim Ahmed Fahal Kevin Kavanagh Wendy W J van de Sande |
author_sort |
Gerard Sheehan |
title |
Proteomic analysis of the processes leading to Madurella mycetomatis grain formation in Galleria mellonella larvae. |
title_short |
Proteomic analysis of the processes leading to Madurella mycetomatis grain formation in Galleria mellonella larvae. |
title_full |
Proteomic analysis of the processes leading to Madurella mycetomatis grain formation in Galleria mellonella larvae. |
title_fullStr |
Proteomic analysis of the processes leading to Madurella mycetomatis grain formation in Galleria mellonella larvae. |
title_full_unstemmed |
Proteomic analysis of the processes leading to Madurella mycetomatis grain formation in Galleria mellonella larvae. |
title_sort |
proteomic analysis of the processes leading to madurella mycetomatis grain formation in galleria mellonella larvae. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0008190 https://doaj.org/article/449e255320ce4cda84c8ce82bbc665c1 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 4, p e0008190 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008190 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008190 https://doaj.org/article/449e255320ce4cda84c8ce82bbc665c1 |
op_doi |
https://doi.org/10.1371/journal.pntd.0008190 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
14 |
container_issue |
4 |
container_start_page |
e0008190 |
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1766338921879306240 |