Rapid Adjustments in Thermal Tolerance and the Metabolome to Daily Environmental Changes – A Field Study on the Arctic Seed Bug Nysius groenlandicus
Laboratory investigations on terrestrial model-species, typically of temperate origin, have demonstrated that terrestrial ectotherms can cope with daily temperature variations through rapid hardening responses. However, few studies have investigated this ability and its physiological basis in the fi...
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Online Access: | https://doi.org/10.3389/fphys.2022.818485 https://doaj.org/article/442f7261a62c4d79aae5bd7ef44b8b8e |
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ftdoajarticles:oai:doaj.org/article:442f7261a62c4d79aae5bd7ef44b8b8e 2023-05-15T15:00:48+02:00 Rapid Adjustments in Thermal Tolerance and the Metabolome to Daily Environmental Changes – A Field Study on the Arctic Seed Bug Nysius groenlandicus Natasja Krog Noer Mathias Hamann Sørensen Hervé Colinet David Renault Simon Bahrndorff Torsten Nygaard Kristensen 2022-02-01T00:00:00Z https://doi.org/10.3389/fphys.2022.818485 https://doaj.org/article/442f7261a62c4d79aae5bd7ef44b8b8e EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fphys.2022.818485/full https://doaj.org/toc/1664-042X 1664-042X doi:10.3389/fphys.2022.818485 https://doaj.org/article/442f7261a62c4d79aae5bd7ef44b8b8e Frontiers in Physiology, Vol 13 (2022) arctic climate change diurnal environmental variation GC-MS metabolomics insects phenotypic plasticity Physiology QP1-981 article 2022 ftdoajarticles https://doi.org/10.3389/fphys.2022.818485 2022-12-31T15:08:13Z Laboratory investigations on terrestrial model-species, typically of temperate origin, have demonstrated that terrestrial ectotherms can cope with daily temperature variations through rapid hardening responses. However, few studies have investigated this ability and its physiological basis in the field. Especially in polar regions, where the temporal and spatial temperature variations can be extreme, are hardening responses expected to be important. Here, we examined diurnal adjustments in heat and cold tolerance in the Greenlandic seed bug Nysius groenlandicus by collecting individuals for thermal assessment at different time points within and across days. We found a significant correlation between observed heat or cold tolerance and the ambient microhabitat temperatures at the time of capture, indicating that N. groenlandicus continuously and within short time-windows respond physiologically to thermal changes and/or other environmental variables in their microhabitats. Secondly, we assessed underlying metabolomic fingerprints using GC-MS metabolomics in a subset of individuals collected during days with either low or high temperature variation. Concentrations of metabolites, including sugars, polyols, and free amino acids varied significantly with time of collection. For instance, we detected elevated sugar levels in animals caught at the lowest daily field temperatures. Polyol concentrations were lower in individuals collected in the morning and evening and higher at midday and afternoon, possibly reflecting changes in temperature. Additionally, changes in concentrations of metabolites associated with energetic metabolism were observed across collection times. Our findings suggest that in these extreme polar environments hardening responses are marked and likely play a crucial role for coping with microhabitat temperature variation on a daily scale, and that metabolite levels are actively altered on a daily basis. Article in Journal/Newspaper Arctic Climate change greenlandic Directory of Open Access Journals: DOAJ Articles Arctic Frontiers in Physiology 13 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
arctic climate change diurnal environmental variation GC-MS metabolomics insects phenotypic plasticity Physiology QP1-981 |
spellingShingle |
arctic climate change diurnal environmental variation GC-MS metabolomics insects phenotypic plasticity Physiology QP1-981 Natasja Krog Noer Mathias Hamann Sørensen Hervé Colinet David Renault Simon Bahrndorff Torsten Nygaard Kristensen Rapid Adjustments in Thermal Tolerance and the Metabolome to Daily Environmental Changes – A Field Study on the Arctic Seed Bug Nysius groenlandicus |
topic_facet |
arctic climate change diurnal environmental variation GC-MS metabolomics insects phenotypic plasticity Physiology QP1-981 |
description |
Laboratory investigations on terrestrial model-species, typically of temperate origin, have demonstrated that terrestrial ectotherms can cope with daily temperature variations through rapid hardening responses. However, few studies have investigated this ability and its physiological basis in the field. Especially in polar regions, where the temporal and spatial temperature variations can be extreme, are hardening responses expected to be important. Here, we examined diurnal adjustments in heat and cold tolerance in the Greenlandic seed bug Nysius groenlandicus by collecting individuals for thermal assessment at different time points within and across days. We found a significant correlation between observed heat or cold tolerance and the ambient microhabitat temperatures at the time of capture, indicating that N. groenlandicus continuously and within short time-windows respond physiologically to thermal changes and/or other environmental variables in their microhabitats. Secondly, we assessed underlying metabolomic fingerprints using GC-MS metabolomics in a subset of individuals collected during days with either low or high temperature variation. Concentrations of metabolites, including sugars, polyols, and free amino acids varied significantly with time of collection. For instance, we detected elevated sugar levels in animals caught at the lowest daily field temperatures. Polyol concentrations were lower in individuals collected in the morning and evening and higher at midday and afternoon, possibly reflecting changes in temperature. Additionally, changes in concentrations of metabolites associated with energetic metabolism were observed across collection times. Our findings suggest that in these extreme polar environments hardening responses are marked and likely play a crucial role for coping with microhabitat temperature variation on a daily scale, and that metabolite levels are actively altered on a daily basis. |
format |
Article in Journal/Newspaper |
author |
Natasja Krog Noer Mathias Hamann Sørensen Hervé Colinet David Renault Simon Bahrndorff Torsten Nygaard Kristensen |
author_facet |
Natasja Krog Noer Mathias Hamann Sørensen Hervé Colinet David Renault Simon Bahrndorff Torsten Nygaard Kristensen |
author_sort |
Natasja Krog Noer |
title |
Rapid Adjustments in Thermal Tolerance and the Metabolome to Daily Environmental Changes – A Field Study on the Arctic Seed Bug Nysius groenlandicus |
title_short |
Rapid Adjustments in Thermal Tolerance and the Metabolome to Daily Environmental Changes – A Field Study on the Arctic Seed Bug Nysius groenlandicus |
title_full |
Rapid Adjustments in Thermal Tolerance and the Metabolome to Daily Environmental Changes – A Field Study on the Arctic Seed Bug Nysius groenlandicus |
title_fullStr |
Rapid Adjustments in Thermal Tolerance and the Metabolome to Daily Environmental Changes – A Field Study on the Arctic Seed Bug Nysius groenlandicus |
title_full_unstemmed |
Rapid Adjustments in Thermal Tolerance and the Metabolome to Daily Environmental Changes – A Field Study on the Arctic Seed Bug Nysius groenlandicus |
title_sort |
rapid adjustments in thermal tolerance and the metabolome to daily environmental changes – a field study on the arctic seed bug nysius groenlandicus |
publisher |
Frontiers Media S.A. |
publishDate |
2022 |
url |
https://doi.org/10.3389/fphys.2022.818485 https://doaj.org/article/442f7261a62c4d79aae5bd7ef44b8b8e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Climate change greenlandic |
genre_facet |
Arctic Climate change greenlandic |
op_source |
Frontiers in Physiology, Vol 13 (2022) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fphys.2022.818485/full https://doaj.org/toc/1664-042X 1664-042X doi:10.3389/fphys.2022.818485 https://doaj.org/article/442f7261a62c4d79aae5bd7ef44b8b8e |
op_doi |
https://doi.org/10.3389/fphys.2022.818485 |
container_title |
Frontiers in Physiology |
container_volume |
13 |
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1766332861101637632 |