Molecular discrimination of Opisthorchis-like eggs from residents in a rural community of central Thailand.
Opisthorchis viverrini infection is a major public health problem in northern and northeastern Thailand. The chronic infection of O. viverrini is related to cholangiocarcinoma which causes high mortality in endemic areas. Therefore, the diagnosis, treatment, control and prevention of O. viverrini in...
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ftdoajarticles:oai:doaj.org/article:4428d8e6ac3d4bb8bc80e1e646700223 2023-05-15T15:16:29+02:00 Molecular discrimination of Opisthorchis-like eggs from residents in a rural community of central Thailand. Saiwasan Buathong Saovanee Leelayoova Mathirut Mungthin Toon Ruang-Areerate Tawee Naaglor Picha Suwannahitatorn Phunlerd Piyaraj Paanjit Taamasri Peerapan Tan-Ariya 2017-11-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0006030 https://doaj.org/article/4428d8e6ac3d4bb8bc80e1e646700223 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5685638?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006030 https://doaj.org/article/4428d8e6ac3d4bb8bc80e1e646700223 PLoS Neglected Tropical Diseases, Vol 11, Iss 11, p e0006030 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0006030 2022-12-31T04:15:37Z Opisthorchis viverrini infection is a major public health problem in northern and northeastern Thailand. The chronic infection of O. viverrini is related to cholangiocarcinoma which causes high mortality in endemic areas. Therefore, the diagnosis, treatment, control and prevention of O. viverrini infection are necessary. The morphology of the egg is very similar to that of other species of human liver flukes (Opisthorchis felineus and Clonorchis sinensis) as well as that of small intestinal flukes in the family Heterophyidae. Thus, molecular characterization is crucially required to discriminate species of Opisthorchis-like eggs in fecal examination.We aimed to determine the prevalence of O. viverrini infection among villagers living in Sanamchaikate District, Chachoengsao Province, in central Thailand, where O. viverrini infection has previously been reported. A total of 2,609 fecal samples were examined for Opisthorchis-like eggs using microscopic examination. PCR-RFLP analysis of the ITS2 region was used to discriminate Opisthorchis-like eggs. The genetic structure of O. viverrini infection was demonstrated using nucleotide sequencing of cytochrome c oxidase subunit I (cox1) and NADH dehydrogenase subunit 1 (nad1). Testing of evolutionary neutrality of the cox1 and nad1 sequences of O. viverrini was performed using Tajima's D tests and Fu's Fs tests. Moreover, the haplotype networks and phylogenetic trees were constructed to study the relationships of O. viverrini isolated from different endemic areas. A high prevalence of O. viverrini infection is still observed in a rural community of Chachoengsao Province, central Thailand. The overall prevalence of Opisthorchis-like eggs using microscopic examination was 16.8%. PCR-RFLP profiles showed the predominant infection of O. viverrini (9.6%) including very low infections of other small intestinal flukes, Haplorchis taichui (0.08%) and Euparyphium albuferensis (0.08%). The genetic structure of O. viverrini populations in central Thailand was also described and ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 11 e0006030 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Saiwasan Buathong Saovanee Leelayoova Mathirut Mungthin Toon Ruang-Areerate Tawee Naaglor Picha Suwannahitatorn Phunlerd Piyaraj Paanjit Taamasri Peerapan Tan-Ariya Molecular discrimination of Opisthorchis-like eggs from residents in a rural community of central Thailand. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Opisthorchis viverrini infection is a major public health problem in northern and northeastern Thailand. The chronic infection of O. viverrini is related to cholangiocarcinoma which causes high mortality in endemic areas. Therefore, the diagnosis, treatment, control and prevention of O. viverrini infection are necessary. The morphology of the egg is very similar to that of other species of human liver flukes (Opisthorchis felineus and Clonorchis sinensis) as well as that of small intestinal flukes in the family Heterophyidae. Thus, molecular characterization is crucially required to discriminate species of Opisthorchis-like eggs in fecal examination.We aimed to determine the prevalence of O. viverrini infection among villagers living in Sanamchaikate District, Chachoengsao Province, in central Thailand, where O. viverrini infection has previously been reported. A total of 2,609 fecal samples were examined for Opisthorchis-like eggs using microscopic examination. PCR-RFLP analysis of the ITS2 region was used to discriminate Opisthorchis-like eggs. The genetic structure of O. viverrini infection was demonstrated using nucleotide sequencing of cytochrome c oxidase subunit I (cox1) and NADH dehydrogenase subunit 1 (nad1). Testing of evolutionary neutrality of the cox1 and nad1 sequences of O. viverrini was performed using Tajima's D tests and Fu's Fs tests. Moreover, the haplotype networks and phylogenetic trees were constructed to study the relationships of O. viverrini isolated from different endemic areas. A high prevalence of O. viverrini infection is still observed in a rural community of Chachoengsao Province, central Thailand. The overall prevalence of Opisthorchis-like eggs using microscopic examination was 16.8%. PCR-RFLP profiles showed the predominant infection of O. viverrini (9.6%) including very low infections of other small intestinal flukes, Haplorchis taichui (0.08%) and Euparyphium albuferensis (0.08%). The genetic structure of O. viverrini populations in central Thailand was also described and ... |
format |
Article in Journal/Newspaper |
author |
Saiwasan Buathong Saovanee Leelayoova Mathirut Mungthin Toon Ruang-Areerate Tawee Naaglor Picha Suwannahitatorn Phunlerd Piyaraj Paanjit Taamasri Peerapan Tan-Ariya |
author_facet |
Saiwasan Buathong Saovanee Leelayoova Mathirut Mungthin Toon Ruang-Areerate Tawee Naaglor Picha Suwannahitatorn Phunlerd Piyaraj Paanjit Taamasri Peerapan Tan-Ariya |
author_sort |
Saiwasan Buathong |
title |
Molecular discrimination of Opisthorchis-like eggs from residents in a rural community of central Thailand. |
title_short |
Molecular discrimination of Opisthorchis-like eggs from residents in a rural community of central Thailand. |
title_full |
Molecular discrimination of Opisthorchis-like eggs from residents in a rural community of central Thailand. |
title_fullStr |
Molecular discrimination of Opisthorchis-like eggs from residents in a rural community of central Thailand. |
title_full_unstemmed |
Molecular discrimination of Opisthorchis-like eggs from residents in a rural community of central Thailand. |
title_sort |
molecular discrimination of opisthorchis-like eggs from residents in a rural community of central thailand. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0006030 https://doaj.org/article/4428d8e6ac3d4bb8bc80e1e646700223 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 11, p e0006030 (2017) |
op_relation |
http://europepmc.org/articles/PMC5685638?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006030 https://doaj.org/article/4428d8e6ac3d4bb8bc80e1e646700223 |
op_doi |
https://doi.org/10.1371/journal.pntd.0006030 |
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PLOS Neglected Tropical Diseases |
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11 |
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11 |
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e0006030 |
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1766346778453475328 |