Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank Bioreactor
Microbial proteases, especially aspartic proteases, are an essential group of enzymes produced from different microorganisms. Microbial proteases have several applications, mainly in the food, beverage, cosmetic, and pharmaceutical industries, due to their efficiency in the processing and in the man...
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ftdoajarticles:oai:doaj.org/article:4418d540371240bd947b7fc6800d9e29 2023-08-27T04:06:06+02:00 Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank Bioreactor Suellen Machado Valker Feitosa Omar Pillaca-Pullo Luciana Lario Lara Sette Adalberto Pessoa Harley Alves 2022-11-01T00:00:00Z https://doi.org/10.3390/bioengineering9110694 https://doaj.org/article/4418d540371240bd947b7fc6800d9e29 EN eng MDPI AG https://www.mdpi.com/2306-5354/9/11/694 https://doaj.org/toc/2306-5354 doi:10.3390/bioengineering9110694 2306-5354 https://doaj.org/article/4418d540371240bd947b7fc6800d9e29 Bioengineering, Vol 9, Iss 694, p 694 (2022) extremophilic psychrophilic proteinase proteolytic bioreactor oxygen transference Technology T Biology (General) QH301-705.5 article 2022 ftdoajarticles https://doi.org/10.3390/bioengineering9110694 2023-08-06T00:41:36Z Microbial proteases, especially aspartic proteases, are an essential group of enzymes produced from different microorganisms. Microbial proteases have several applications, mainly in the food, beverage, cosmetic, and pharmaceutical industries, due to their efficiency in the processing and in the manufacturing stages. The yeast Rhodotorula mucilaginosa CBMAI 1528 was isolated from the Antarctic environment and was previously reported to have higher extracellular aspartic protease production. In addition, advances in the operational conditions of bioreactors for enzyme production are important to reduce the gap associated with scaling−up processes. This is the first study that evaluates the influence of oxygen transference ( k L a ) on the protease production of R. mucilaginosa yeast. To that end, batch cultures were created in a stirred tank bioreactor using Sabouraud dextrose broth at 25 °C for 72 h under k L a values from 18 to 135 h −1 . The results show that k L a (121 h −1 ) obtained at 500 rpm and 1.5 vvm plays an important role in protease production (124.9 U/mL) and productivity (6.784 U/L.h) as well as biomass (10.4 g/L), μ max (0.14 h −1 ) and Y x/s (0.484 g/g). In conclusion, R. mucilaginosa showed high yield production in aerobic culture with the efficiency of protease expression and secretion influenced by k L a . In this sense, our results could be used for further industrial investment. Article in Journal/Newspaper Antarc* Antarctic Directory of Open Access Journals: DOAJ Articles Antarctic The Antarctic Bioengineering 9 11 694 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
extremophilic psychrophilic proteinase proteolytic bioreactor oxygen transference Technology T Biology (General) QH301-705.5 |
spellingShingle |
extremophilic psychrophilic proteinase proteolytic bioreactor oxygen transference Technology T Biology (General) QH301-705.5 Suellen Machado Valker Feitosa Omar Pillaca-Pullo Luciana Lario Lara Sette Adalberto Pessoa Harley Alves Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank Bioreactor |
topic_facet |
extremophilic psychrophilic proteinase proteolytic bioreactor oxygen transference Technology T Biology (General) QH301-705.5 |
description |
Microbial proteases, especially aspartic proteases, are an essential group of enzymes produced from different microorganisms. Microbial proteases have several applications, mainly in the food, beverage, cosmetic, and pharmaceutical industries, due to their efficiency in the processing and in the manufacturing stages. The yeast Rhodotorula mucilaginosa CBMAI 1528 was isolated from the Antarctic environment and was previously reported to have higher extracellular aspartic protease production. In addition, advances in the operational conditions of bioreactors for enzyme production are important to reduce the gap associated with scaling−up processes. This is the first study that evaluates the influence of oxygen transference ( k L a ) on the protease production of R. mucilaginosa yeast. To that end, batch cultures were created in a stirred tank bioreactor using Sabouraud dextrose broth at 25 °C for 72 h under k L a values from 18 to 135 h −1 . The results show that k L a (121 h −1 ) obtained at 500 rpm and 1.5 vvm plays an important role in protease production (124.9 U/mL) and productivity (6.784 U/L.h) as well as biomass (10.4 g/L), μ max (0.14 h −1 ) and Y x/s (0.484 g/g). In conclusion, R. mucilaginosa showed high yield production in aerobic culture with the efficiency of protease expression and secretion influenced by k L a . In this sense, our results could be used for further industrial investment. |
format |
Article in Journal/Newspaper |
author |
Suellen Machado Valker Feitosa Omar Pillaca-Pullo Luciana Lario Lara Sette Adalberto Pessoa Harley Alves |
author_facet |
Suellen Machado Valker Feitosa Omar Pillaca-Pullo Luciana Lario Lara Sette Adalberto Pessoa Harley Alves |
author_sort |
Suellen Machado |
title |
Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank Bioreactor |
title_short |
Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank Bioreactor |
title_full |
Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank Bioreactor |
title_fullStr |
Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank Bioreactor |
title_full_unstemmed |
Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank Bioreactor |
title_sort |
effects of oxygen transference on protease production by rhodotorula mucilaginosa cbmai 1528 in a stirred tank bioreactor |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/bioengineering9110694 https://doaj.org/article/4418d540371240bd947b7fc6800d9e29 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Bioengineering, Vol 9, Iss 694, p 694 (2022) |
op_relation |
https://www.mdpi.com/2306-5354/9/11/694 https://doaj.org/toc/2306-5354 doi:10.3390/bioengineering9110694 2306-5354 https://doaj.org/article/4418d540371240bd947b7fc6800d9e29 |
op_doi |
https://doi.org/10.3390/bioengineering9110694 |
container_title |
Bioengineering |
container_volume |
9 |
container_issue |
11 |
container_start_page |
694 |
_version_ |
1775346847756320768 |