Hematological changes in sheep inoculated with natural and Cobalt60-irradiated Crotalus durissus terrificus venom (Laurenti, 1768)
Natural (NV) and Cobalto60-irradiated (IrV) Crotalus durissus terrificus venom were used to evaluate serum production capacity of sheep and possible hematological and biochemical effects. Freeze-dried venom aliquots were diluted in acidified saline solution (NaCl 150 mM, pH 3.0) and irradiated by a...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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Online Access: | https://doi.org/10.1590/S1678-91992004000100004 https://doaj.org/article/4415c4c54eaf4dbe9c92cc5e1f14d1e9 |
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ftdoajarticles:oai:doaj.org/article:4415c4c54eaf4dbe9c92cc5e1f14d1e9 2023-05-15T15:17:53+02:00 Hematological changes in sheep inoculated with natural and Cobalt60-irradiated Crotalus durissus terrificus venom (Laurenti, 1768) D. P. Netto S. B. Chiacchio P. L. Bicudo A. A. Alfieri M. R. S. Balarim N. Nascimento 2004-01-01T00:00:00Z https://doi.org/10.1590/S1678-91992004000100004 https://doaj.org/article/4415c4c54eaf4dbe9c92cc5e1f14d1e9 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000100004 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992004000100004 1678-9199 https://doaj.org/article/4415c4c54eaf4dbe9c92cc5e1f14d1e9 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 10, Iss 1, Pp 34-52 (2004) hematology gamma radiation irradiated crotalic venom crotalic antiserum ovine Crotalus durissus terrificus Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2004 ftdoajarticles https://doi.org/10.1590/S1678-91992004000100004 2022-12-31T09:08:52Z Natural (NV) and Cobalto60-irradiated (IrV) Crotalus durissus terrificus venom were used to evaluate serum production capacity of sheep and possible hematological and biochemical effects. Freeze-dried venom aliquots were diluted in acidified saline solution (NaCl 150 mM, pH 3.0) and irradiated by a Cobalt 60 source at a dose of 5.54 x 102 Gy/h and a concentration of 2.000 Gy. Twelve sheep were divided into two groups of six animals. One group received irradiated venom (IrV) and the other natural venom (NV). Three antigen doses (venom) were administered at monthly intervals. Blood samples were collected weekly for analysis of serum neutralization potency and capacity, complete blood count (CBC), total plasma protein, fibrinogen, albumin, and globulin. At the end of the experiment, the animals were challenged with a LD50 for sheep and showed no signs of envenoming. The two groups did not present clinical alterations. Results of the total leukocyte count did not present interaction or time factor effect for both groups, but there was a different action between them, with the NV group presenting more cells than the IrV group. The leukocyte increase to 13,000/ml indicates that slight leukocytosis occurred in the week after the first inoculation in the NV group. There was no statistically significant difference between groups in the absolute count of segmented neutrophils, eosinophils, and lymphocytes but there were statistically significant oscillations in values at the different collecting times. The NV group presented an increase in the absolute neutrophil count after the first inoculation that persisted for 5 weeks. In the IrV group, the increase in neutrophils occurred only in the first week returning to normal in the following weeks. The alterations in the neutrophil count are indicative of systemic inflammatory response related to cytokine release; response was more marked in the NV group, showing its greater toxicity. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 10 1 34 52 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
hematology gamma radiation irradiated crotalic venom crotalic antiserum ovine Crotalus durissus terrificus Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
hematology gamma radiation irradiated crotalic venom crotalic antiserum ovine Crotalus durissus terrificus Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 D. P. Netto S. B. Chiacchio P. L. Bicudo A. A. Alfieri M. R. S. Balarim N. Nascimento Hematological changes in sheep inoculated with natural and Cobalt60-irradiated Crotalus durissus terrificus venom (Laurenti, 1768) |
topic_facet |
hematology gamma radiation irradiated crotalic venom crotalic antiserum ovine Crotalus durissus terrificus Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Natural (NV) and Cobalto60-irradiated (IrV) Crotalus durissus terrificus venom were used to evaluate serum production capacity of sheep and possible hematological and biochemical effects. Freeze-dried venom aliquots were diluted in acidified saline solution (NaCl 150 mM, pH 3.0) and irradiated by a Cobalt 60 source at a dose of 5.54 x 102 Gy/h and a concentration of 2.000 Gy. Twelve sheep were divided into two groups of six animals. One group received irradiated venom (IrV) and the other natural venom (NV). Three antigen doses (venom) were administered at monthly intervals. Blood samples were collected weekly for analysis of serum neutralization potency and capacity, complete blood count (CBC), total plasma protein, fibrinogen, albumin, and globulin. At the end of the experiment, the animals were challenged with a LD50 for sheep and showed no signs of envenoming. The two groups did not present clinical alterations. Results of the total leukocyte count did not present interaction or time factor effect for both groups, but there was a different action between them, with the NV group presenting more cells than the IrV group. The leukocyte increase to 13,000/ml indicates that slight leukocytosis occurred in the week after the first inoculation in the NV group. There was no statistically significant difference between groups in the absolute count of segmented neutrophils, eosinophils, and lymphocytes but there were statistically significant oscillations in values at the different collecting times. The NV group presented an increase in the absolute neutrophil count after the first inoculation that persisted for 5 weeks. In the IrV group, the increase in neutrophils occurred only in the first week returning to normal in the following weeks. The alterations in the neutrophil count are indicative of systemic inflammatory response related to cytokine release; response was more marked in the NV group, showing its greater toxicity. |
format |
Article in Journal/Newspaper |
author |
D. P. Netto S. B. Chiacchio P. L. Bicudo A. A. Alfieri M. R. S. Balarim N. Nascimento |
author_facet |
D. P. Netto S. B. Chiacchio P. L. Bicudo A. A. Alfieri M. R. S. Balarim N. Nascimento |
author_sort |
D. P. Netto |
title |
Hematological changes in sheep inoculated with natural and Cobalt60-irradiated Crotalus durissus terrificus venom (Laurenti, 1768) |
title_short |
Hematological changes in sheep inoculated with natural and Cobalt60-irradiated Crotalus durissus terrificus venom (Laurenti, 1768) |
title_full |
Hematological changes in sheep inoculated with natural and Cobalt60-irradiated Crotalus durissus terrificus venom (Laurenti, 1768) |
title_fullStr |
Hematological changes in sheep inoculated with natural and Cobalt60-irradiated Crotalus durissus terrificus venom (Laurenti, 1768) |
title_full_unstemmed |
Hematological changes in sheep inoculated with natural and Cobalt60-irradiated Crotalus durissus terrificus venom (Laurenti, 1768) |
title_sort |
hematological changes in sheep inoculated with natural and cobalt60-irradiated crotalus durissus terrificus venom (laurenti, 1768) |
publisher |
SciELO |
publishDate |
2004 |
url |
https://doi.org/10.1590/S1678-91992004000100004 https://doaj.org/article/4415c4c54eaf4dbe9c92cc5e1f14d1e9 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 10, Iss 1, Pp 34-52 (2004) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000100004 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992004000100004 1678-9199 https://doaj.org/article/4415c4c54eaf4dbe9c92cc5e1f14d1e9 |
op_doi |
https://doi.org/10.1590/S1678-91992004000100004 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
10 |
container_issue |
1 |
container_start_page |
34 |
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52 |
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