Stage- and gender-specific proteomic analysis of Brugia malayi excretory-secretory products.
INTRODUCTION: While we lack a complete understanding of the molecular mechanisms by which parasites establish and achieve protection from host immune responses, it is accepted that many of these processes are mediated by products, primarily proteins, released from the parasite. Parasitic nematodes o...
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ftdoajarticles:oai:doaj.org/article:43a2c60e15d644aea6ac212625161f84 2023-05-15T15:12:34+02:00 Stage- and gender-specific proteomic analysis of Brugia malayi excretory-secretory products. Yovany Moreno Timothy G Geary 2008-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0000326 https://doaj.org/article/43a2c60e15d644aea6ac212625161f84 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC2569413?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0000326 https://doaj.org/article/43a2c60e15d644aea6ac212625161f84 PLoS Neglected Tropical Diseases, Vol 2, Iss 10, p e326 (2008) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2008 ftdoajarticles https://doi.org/10.1371/journal.pntd.0000326 2022-12-31T13:05:13Z INTRODUCTION: While we lack a complete understanding of the molecular mechanisms by which parasites establish and achieve protection from host immune responses, it is accepted that many of these processes are mediated by products, primarily proteins, released from the parasite. Parasitic nematodes occur in different life stages and anatomical compartments within the host. Little is known about the composition and variability of products released at different developmental stages and their contribution to parasite survival and progression of the infection. METHODOLOGY/PRINCIPAL FINDINGS: To gain a deeper understanding on these aspects, we collected and analyzed through 1D-SDS PAGE and LC-MS/MS the Excretory-Secretory Products (ESP) of adult female, adult male and microfilariae of the filarial nematode Brugia malayi, one of the etiological agents of human lymphatic filariasis. This proteomic analysis led to the identification of 228 proteins. The list includes 76 proteins with unknown function as well as also proteins with potential immunoregulatory properties, such as protease inhibitors, cytokine homologues and carbohydrate-binding proteins. Larval and adult ESP differed in composition. Only 32 proteins were shared between all three stages/genders. Consistent with this observation, different gene ontology profiles were associated with the different ESP. CONCLUSIONS/SIGNIFICANCE: A comparative analysis of the proteins released in vitro by different forms of a parasitic nematode dwelling in the same host is presented. The catalog of secreted proteins reflects different stage- and gender-specific related processes and different strategies of immune evasion, providing valuable insights on the contribution of each form of the parasite for establishing the host-parasite interaction. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 2 10 e326 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Yovany Moreno Timothy G Geary Stage- and gender-specific proteomic analysis of Brugia malayi excretory-secretory products. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
INTRODUCTION: While we lack a complete understanding of the molecular mechanisms by which parasites establish and achieve protection from host immune responses, it is accepted that many of these processes are mediated by products, primarily proteins, released from the parasite. Parasitic nematodes occur in different life stages and anatomical compartments within the host. Little is known about the composition and variability of products released at different developmental stages and their contribution to parasite survival and progression of the infection. METHODOLOGY/PRINCIPAL FINDINGS: To gain a deeper understanding on these aspects, we collected and analyzed through 1D-SDS PAGE and LC-MS/MS the Excretory-Secretory Products (ESP) of adult female, adult male and microfilariae of the filarial nematode Brugia malayi, one of the etiological agents of human lymphatic filariasis. This proteomic analysis led to the identification of 228 proteins. The list includes 76 proteins with unknown function as well as also proteins with potential immunoregulatory properties, such as protease inhibitors, cytokine homologues and carbohydrate-binding proteins. Larval and adult ESP differed in composition. Only 32 proteins were shared between all three stages/genders. Consistent with this observation, different gene ontology profiles were associated with the different ESP. CONCLUSIONS/SIGNIFICANCE: A comparative analysis of the proteins released in vitro by different forms of a parasitic nematode dwelling in the same host is presented. The catalog of secreted proteins reflects different stage- and gender-specific related processes and different strategies of immune evasion, providing valuable insights on the contribution of each form of the parasite for establishing the host-parasite interaction. |
format |
Article in Journal/Newspaper |
author |
Yovany Moreno Timothy G Geary |
author_facet |
Yovany Moreno Timothy G Geary |
author_sort |
Yovany Moreno |
title |
Stage- and gender-specific proteomic analysis of Brugia malayi excretory-secretory products. |
title_short |
Stage- and gender-specific proteomic analysis of Brugia malayi excretory-secretory products. |
title_full |
Stage- and gender-specific proteomic analysis of Brugia malayi excretory-secretory products. |
title_fullStr |
Stage- and gender-specific proteomic analysis of Brugia malayi excretory-secretory products. |
title_full_unstemmed |
Stage- and gender-specific proteomic analysis of Brugia malayi excretory-secretory products. |
title_sort |
stage- and gender-specific proteomic analysis of brugia malayi excretory-secretory products. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2008 |
url |
https://doi.org/10.1371/journal.pntd.0000326 https://doaj.org/article/43a2c60e15d644aea6ac212625161f84 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 2, Iss 10, p e326 (2008) |
op_relation |
http://europepmc.org/articles/PMC2569413?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0000326 https://doaj.org/article/43a2c60e15d644aea6ac212625161f84 |
op_doi |
https://doi.org/10.1371/journal.pntd.0000326 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
2 |
container_issue |
10 |
container_start_page |
e326 |
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1766343232296321024 |