Amino acid δ13C and δ15N fingerprinting of sea ice and pelagic algae in Canadian Arctic and Subarctic Seas

• Published in: Frontiers in Marine Science
• Main Authors: Shao-Min Chen, Peta Mudie, Owen A. Sherwood
• Format: Article in Journal/Newspaper
• Language: English
• Published: Frontiers Media S.A. 2022
• Subjects: isotope ecology; compound specific; stable isotopes; amino acid; phytoplankton; biomarker; Science; Q; General. Including nature conservation; geographical distribution; QH1-199.5; Arctic; Nunavut; Canada; ice algae; Phytoplankton; Sea ice; Subarctic
• Online Access: https://doi.org/10.3389/fmars.2022.976908; https://doaj.org/article/4395ba26203d443283ea32752386fae2

The on-going decline in Arctic sea ice represents a significant loss of habitat for sea ice algae, which are a major contributor to primary production in the Arctic. Data on sea ice algal production is limited due to difficulties in both accessing sea-ice and sampling under-ice algae. Compound-specific isotope analysis (CSIA) of amino acids (AAs) is emerging as a powerful tool to trace element origins and biogeochemical processes in marine food webs and may address the knowledge gaps in sea ice algal productivity dynamics. Here we measured δ13C-AA and δ15N-AA in natural communities of sea ice and pelagic algae collected from regions offshore Labrador and Nunavut, Canada. Significant difference in δ13C-AA patterns between sea ice and pelagic algae was observed in different size classes. This difference was further supported by multivariate analyses based on normalized δ13C of essential amino acids (EAAs), which demonstrated a clear separation between sea ice and pelagic algae. Beta (β) values and trophic position (TP) calculated from δ15N of Glutamic Acid and Phenylalanine (Phe) and ∑V parameter for microbial resynthesis indicated a slightly higher heterotrophic biomass in pelagic and sea ice samples as compared to cultured samples. This finding is consistent with the Phe-normalized δ15N of Alanine and Threonine, which provided better separations between sea ice/pelagic algae and other end-member groups. Overall, our study provides first insights into the potential differences in δ13C-AA and δ15N-AA patterns between sea ice and pelagic algae and suggests carbon of sea ice origins may be distinguished from pelagic sources using CSIA-AA approach. These observations highlight the potential of CSIA-AA to estimate proportional contributions of sea ice and pelagic algae to export production and efficiency of benthic-pelagic coupling in polar marine environments.