Online service for interpretation of the resistance prediction results to bedaquiline by the molecular data
Background. Bedaquiline is a new and promising anti-tuberculosis drug, but longterm use requires resistance. This is due to mutations in the atpE and mmpR genes in M. tuberculosis (MBT).The aim of the research was to test a system for automated interpretation of results for predicting resistance to...
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Scientific Сentre for Family Health and Human Reproduction Problems
2024
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ftdoajarticles:oai:doaj.org/article:43874dd0b9b24601ab54936e5317e9db 2024-10-13T14:11:19+00:00 Online service for interpretation of the resistance prediction results to bedaquiline by the molecular data V. V. Sinkov I. G. Kondratov O. B. Ogarkov S. N. Zhdanova A. P. Noskov P. A. Khromova E. A. Orlova A. V. Labygina L. V. Rychkova L. I. Kolesnikova 2024-01-01T00:00:00Z https://doi.org/10.29413/ABS.2023-8.6.11 https://doaj.org/article/43874dd0b9b24601ab54936e5317e9db RU rus Scientific Сentre for Family Health and Human Reproduction Problems https://www.actabiomedica.ru/jour/article/view/4498 https://doaj.org/toc/2541-9420 https://doaj.org/toc/2587-9596 https://doaj.org/article/43874dd0b9b24601ab54936e5317e9db Acta Biomedica Scientifica, Vol 8, Iss 6, Pp 124-129 (2024) bedaquiline sequencing resistance genes atpe mmpr mmpl5 mmps5 rv0678 rv1979c pepq Science Q article 2024 ftdoajarticles https://doi.org/10.29413/ABS.2023-8.6.11 2024-10-02T16:07:15Z Background. Bedaquiline is a new and promising anti-tuberculosis drug, but longterm use requires resistance. This is due to mutations in the atpE and mmpR genes in M. tuberculosis (MBT).The aim of the research was to test a system for automated interpretation of results for predicting resistance to bedaquiline by the molecular data.Materials and methods. DNA was isolated from strains of M. tuberculosis in the Irkutsk region and Yakutia. The total quantity of DNA samples was 27 strains from Yakutia and 21 strains from the Irkutsk region. The study of MBT genomes was carried out on the DNA previously obtained by the authors in the territories of the Irkutsk region (n = 5), Yakutia (n = 4), Buryatia (n = 3), Zabaykalskiy kray (n = 4) and the Far East (n = 8). We used the BSATool program to detect bedaquiline resistance based on Sanger and genomic data. Sanger sequencing analyzed the atpE and mmpR genes, and whole genome sequencing examined mutations in the same sequences, as well as additionally in mmpL5, mmpS5, Rv0678, Rv1979c, and pepQ.Results. Complete agreement between the phenotypic and genotypic analysis of resistance to bedaquiline was found for three strains from Yakutia. One genome with significant mutations to bedaquiline was identified. A conclusion was made about the importance of molecular analysis of target genes with subsequent detection of resistance to bedaquiline in silico. Article in Journal/Newspaper Yakutia Directory of Open Access Journals: DOAJ Articles Acta Biomedica Scientifica 8 6 124 129 |
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bedaquiline sequencing resistance genes atpe mmpr mmpl5 mmps5 rv0678 rv1979c pepq Science Q |
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bedaquiline sequencing resistance genes atpe mmpr mmpl5 mmps5 rv0678 rv1979c pepq Science Q V. V. Sinkov I. G. Kondratov O. B. Ogarkov S. N. Zhdanova A. P. Noskov P. A. Khromova E. A. Orlova A. V. Labygina L. V. Rychkova L. I. Kolesnikova Online service for interpretation of the resistance prediction results to bedaquiline by the molecular data |
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bedaquiline sequencing resistance genes atpe mmpr mmpl5 mmps5 rv0678 rv1979c pepq Science Q |
description |
Background. Bedaquiline is a new and promising anti-tuberculosis drug, but longterm use requires resistance. This is due to mutations in the atpE and mmpR genes in M. tuberculosis (MBT).The aim of the research was to test a system for automated interpretation of results for predicting resistance to bedaquiline by the molecular data.Materials and methods. DNA was isolated from strains of M. tuberculosis in the Irkutsk region and Yakutia. The total quantity of DNA samples was 27 strains from Yakutia and 21 strains from the Irkutsk region. The study of MBT genomes was carried out on the DNA previously obtained by the authors in the territories of the Irkutsk region (n = 5), Yakutia (n = 4), Buryatia (n = 3), Zabaykalskiy kray (n = 4) and the Far East (n = 8). We used the BSATool program to detect bedaquiline resistance based on Sanger and genomic data. Sanger sequencing analyzed the atpE and mmpR genes, and whole genome sequencing examined mutations in the same sequences, as well as additionally in mmpL5, mmpS5, Rv0678, Rv1979c, and pepQ.Results. Complete agreement between the phenotypic and genotypic analysis of resistance to bedaquiline was found for three strains from Yakutia. One genome with significant mutations to bedaquiline was identified. A conclusion was made about the importance of molecular analysis of target genes with subsequent detection of resistance to bedaquiline in silico. |
format |
Article in Journal/Newspaper |
author |
V. V. Sinkov I. G. Kondratov O. B. Ogarkov S. N. Zhdanova A. P. Noskov P. A. Khromova E. A. Orlova A. V. Labygina L. V. Rychkova L. I. Kolesnikova |
author_facet |
V. V. Sinkov I. G. Kondratov O. B. Ogarkov S. N. Zhdanova A. P. Noskov P. A. Khromova E. A. Orlova A. V. Labygina L. V. Rychkova L. I. Kolesnikova |
author_sort |
V. V. Sinkov |
title |
Online service for interpretation of the resistance prediction results to bedaquiline by the molecular data |
title_short |
Online service for interpretation of the resistance prediction results to bedaquiline by the molecular data |
title_full |
Online service for interpretation of the resistance prediction results to bedaquiline by the molecular data |
title_fullStr |
Online service for interpretation of the resistance prediction results to bedaquiline by the molecular data |
title_full_unstemmed |
Online service for interpretation of the resistance prediction results to bedaquiline by the molecular data |
title_sort |
online service for interpretation of the resistance prediction results to bedaquiline by the molecular data |
publisher |
Scientific Сentre for Family Health and Human Reproduction Problems |
publishDate |
2024 |
url |
https://doi.org/10.29413/ABS.2023-8.6.11 https://doaj.org/article/43874dd0b9b24601ab54936e5317e9db |
genre |
Yakutia |
genre_facet |
Yakutia |
op_source |
Acta Biomedica Scientifica, Vol 8, Iss 6, Pp 124-129 (2024) |
op_relation |
https://www.actabiomedica.ru/jour/article/view/4498 https://doaj.org/toc/2541-9420 https://doaj.org/toc/2587-9596 https://doaj.org/article/43874dd0b9b24601ab54936e5317e9db |
op_doi |
https://doi.org/10.29413/ABS.2023-8.6.11 |
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Acta Biomedica Scientifica |
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8 |
container_issue |
6 |
container_start_page |
124 |
op_container_end_page |
129 |
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1812819003413889024 |